湖北农业科学 ›› 2022, Vol. 61 ›› Issue (9): 114-117.doi: 10.14088/j.cnki.issn0439-8114.2022.09.022

• 检测分析 • 上一篇    下一篇

HPLC法同时测定凉粉草中四种成分的含量

莫颖华, 冯白茹, 毛一中   

  1. 惠州卫生职业技术学院,广东 惠州 516025
  • 收稿日期:2021-10-28 出版日期:2022-05-10 发布日期:2022-05-26
  • 作者简介:莫颖华(1982-),女,广东阳江人,讲师,硕士,主要从事中药及其制剂的质量分析研究,(电话)18319923684(电子信箱)89575870@qq.com。
  • 基金资助:
    广东省普通高校特色创新类项目(2018GKTSCX097)

Simultaneous determination of the contents of four components in Mesona chinensis Benth. by HPLC

MO Ying-hua, FENG Bai-ru, MAO Yi-zhong   

  1. Huizhou Health Sciences Polytechnic, Huizhou 516025, Guangdong, China
  • Received:2021-10-28 Online:2022-05-10 Published:2022-05-26

摘要: 采用HPLC法,用乙腈∶0.1%甲酸进行梯度洗脱,检测波长254 nm,流速1.0 mL/min,进样量10 μL,同时测定凉粉草(Mesona chinensis Benth.)中的咖啡酸、异槲皮苷、紫云英苷和迷迭香酸4种成分含量,并进行系统适用性考察。结果显示,咖啡酸、异槲皮苷、紫云英苷、迷迭香酸分别在34.297~171.480 μg/mL、11.172~55.860 μg/mL、16.386~81.928 μg/mL、13.394~66.970 μg/mL浓度范围内线性关系良好,r分别为0.999 9、1.000 0、1.000 0、0.999 7;精密度、重复性、稳定性试验结果的RSD均小于3.00%;9次试验的4种成分相对应的平均回收率均大于95.00%,RSD均小于3.00%。

关键词: HPLC, 凉粉草(Mesona chinensis Benth.), 含量测定

Abstract: HPLC method was used with acetonitrile∶0.1% formic acid as gradient elution. The detection wavelength was 254 nm, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. At the same time, the contents of four components in Mesona chinensis Benth.,including caffeic acid, isoquercitrin, astragaloside and rosmarinic acid were determined, and the applicability of the system was investigated. The results showed that caffeic acid, isoquercitrin, astragaloside and rosmarinic acid had a good linear relationships in the concentration ranges of 34.297~171.480 μg/mL,11.172~55.860 μg/mL,16.386~81.928 μg/mL and 13.394~66.970 μg/mL, respectively. The correlation coefficients were 0.999 9, 1.000 0, 1.000 0 and 0.999 7, respectively. RSD of precision, repeatability and stability test results were all less than 3.00%. The average recoveries of the four components in the nine tests were all greater than 95.00% and RSD was less than 3.00%.

Key words: HPLC, Mesona chinensis Benth., content determination

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