湖北农业科学 ›› 2019, Vol. 58 ›› Issue (3): 57-60.doi: 10.14088/j.cnki.issn0439-8114.2019.03.016

• 资源·环境 • 上一篇    下一篇

一株冬虫夏草菌的鉴定及其抗氧化特性研究

丁浩, 安超, 薛文娇, 马赛箭, 上官亦卿   

  1. 陕西省微生物研究所,西安 710043
  • 收稿日期:2018-11-27 出版日期:2019-02-10 发布日期:2019-11-29
  • 通讯作者: 薛文娇,女,副研究员,博士,主要从事生物化工研究,(电子信箱)x-wenjiao@163.com。
  • 作者简介:丁 浩(1990-),男,陕西西安人,助理工程师,主要从事发酵工程学研究,(电话)15109254081(电子信箱)dhwj2010@qq.com
  • 基金资助:
    陕西省科学院科技计划项目(2013K-06)

Identification and Antioxidant Property of a Fungus Isolated from Cordyceps sinensis

DING Hao, AN Chao, XUE Wen-jiao, MA Sai-jian, SHANGGUAN Yi-qing   

  1. Microbiology Institute of Shaanxi,Xi’an 710043,China
  • Received:2018-11-27 Online:2019-02-10 Published:2019-11-29

摘要: 对分离自野生冬虫夏草(Cordyceps sinensis)的一株真菌F4539进行形态学和分子生物学鉴定,并对其发酵产物进行抗氧化活性研究。结果表明,F4539(Genbank号为KU359775)与Hirsutella sinensis(AJ309355)具有99%的相似性,初步确认F4539为中华被毛孢(Hirsutella sinensis)。抗氧化活性试验结果表明,F4539发酵物20 g/L乙醇提取液的DPPH抗氧化活性为75.39%,高于同浓度国产某商品化冬虫夏草菌菌丝体胶囊乙醇提取物的抗氧化活性,进一步研究表明,F4395发酵物和国产某商品清除DPPH自由基的IC50分别为5.96 g/L和11.61 g/L,即F4539发酵提取物清除DPPH自由基的能力强于国产某商品。

关键词: 冬虫夏草(Cordyceps sinensis), 中华被毛孢(Hirsutella sinensis), 抗氧化

Abstract: A fungus numbered F4539 was isolated from wild Cordyceps sinensis and identified by morphology and molecular biological identification, in addition, the antioxidant activity for the fermentation products were studied. The results demonstrated that F4539(GenbankKU359775) had relatively high similarity in evolution with Hirsutella sinensis(AJ309355), it was confirmed that F4539 was Hirsutella sinensis; Antioxidant activity tests showed that the DPPH antioxidant activity was 75.39% with a concentration of 20 g/L ethanol extract from F4539 fermentation products, which was higher than the same concentration of ethanol extracts from the certain commercial Cordyceps sinensis mycelium capsule. Further research showed that the IC50 of F4539 fermentation products and commercial Cordyceps sinensis mycelium capsule were 5.96 g/L and 11.61 g/L respectively. It was shown that the antioxidant activity was higher in F4539 fermentation products than the commercial Cordyceps sinensis mycelium capsule.

Key words: Cordyceps sinensis, Hirsutella sinensis, antioxidant

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