湖北农业科学 ›› 2022, Vol. 61 ›› Issue (7): 83-88.doi: 10.14088/j.cnki.issn0439-8114.2022.07.015

• 园艺·特产 • 上一篇    下一篇

氯化铯处理对菠菜叶片光系统Ⅱ活性的影响

徐静, 王超, 伍勇, 吴秀丽, 马泽刚   

  1. 四川卫生康复职业学院,四川 自贡 643000
  • 收稿日期:2021-01-25 出版日期:2022-04-10 发布日期:2022-05-04
  • 作者简介:徐 静(1988-),女,内蒙古呼伦贝尔人,助教,硕士,主要从事核废物生物效应、植物逆境生理的研究工作,(电话)0813-8283566(电子信箱)438150081@qq.com。
  • 基金资助:
    自贡市重点科技计划项目(2020YGJC10); 四川卫生康复职业学院校级项目(CWKY-2020Y-05); 四川卫生康复职业学院生物资源开发与利用创新团队项目(CWKY-TD20-06)

Effect of cesium chloride treatment on photosystem II activity of spinach leaves

XU Jing, WANG Chao, WU Yong, WU Xiu-li, MA Ze-gang   

  1. Sichuan Vocational College of Health and Rehabilit,Zigong 643000,Sichuan,China
  • Received:2021-01-25 Online:2022-04-10 Published:2022-05-04

摘要: 为进一步揭示Cs对植物光合作用的影响机理,采用不同浓度的CsCl和石英砂培处理菠菜(Spinacia oleracea L.)幼苗,利用快速叶绿素荧光诱导动力学分析手段,探究Cs胁迫后菠菜叶片光系统Ⅱ(PSII)的受损部位、受损程度及光合电子传递情况。结果表明,当CsCl处理浓度为1~5 mmol/L时,菠菜PSII反应中心供体侧放氧复合体(OEC)受到一定伤害,但不显著,与此同时,φPo、φEo、Ψo参数值上升,说明低浓度处理下菠菜通过调节有活性的反应中心的开放程度升高,促使PSII光合活性增大,从而适应外界环境的变化;当CsCl处理浓度大于10 mmol/L时,CsCl胁迫既损伤了菠菜叶片PSII供体侧OEC,同时也对PSII的反应中心和受体侧造成伤害,从而阻碍光合电子的传递。

关键词: 铯, 菠菜(Spinacia oleracea L.), 光合机构, 快速叶绿素荧光诱导动力学, JIP-测定

Abstract: In order to reveal the effect mechanism of Cs on plant photosynthesis, different concentrations of CsCl were used to treat spinach seedlings which were cultured in quartz sand. The chlorophyll fluorescence induction kinetic analysis method were used to explore damaged position, damaged degree and photosynthetic electron transfer of spinach leaf PSII after Cs stress. The results showed that the OEC on the donor side of the PSII reaction center of spinach suffered slight damage when the CsCl concentration is 1~5 mmol/L. In order to adapt to the change of external environment, the opening degree of active reaction centers in spinach were increased under low concentration treatment, which promotes the increase of PSII photosynthetic activity were reflected by the rised parameters of φPoEoo. Photosynthetic electron transport were blocked because of the damage on the PSII donor side OEC of spinach leaves, reaction center and receptor side of PSII when the concentrations of CsCl reach 10 mmol/L.

Key words: cesium, spinach, photosynthetic machinery, fast chlorophyll fluorescence induction kinetics, JIP-test

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