湖北农业科学 ›› 2022, Vol. 61 ›› Issue (4): 160-163.doi: 10.14088/j.cnki.issn0439-8114.2022.04.032

• 生物工程 • 上一篇    下一篇

京山地区对节白蜡与“京翠”亲缘关系的cpSSR分析

金宇晨1, 柴隆龙1, 马倩2, 熊燕飞1, 张建坤1, 陈碧峰1   

  1. 1.武汉理工大学化学化工与生命科学学院,武汉 430014;
    2.湖北森宝园艺有限公司,湖北 荆门 431815
  • 收稿日期:2021-10-11 出版日期:2022-02-25 发布日期:2022-03-18
  • 通讯作者: 陈碧峰(1984-),男,湖北武汉人,副教授,博士,主要从事遗传学研究工作,(电子信箱)cbifeng@whut.edu.cn。
  • 作者简介:金宇晨(1998-),男,湖北武汉人,在读硕士研究生,研究方向为遗传学,(电话)15129297031(电子信箱)1579009018@qq.com。
  • 基金资助:
    中央高校基本科研业务费专项基金(2020IB029)

CpSSR analysis of the genetic relationship between Fraxinus hupehensis and “Jingcui” in Jingshan area

JIN Yu-chen1, CHAI Long-long1, MA Qian2, XIONG Yan-fei1, ZHANG Jian-kun1, CHEN Bi-feng1   

  1. 1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430014, China;
    2. Hubei Senbao Horticulture Co., Ltd., Jingmen 431815, Hubei,China
  • Received:2021-10-11 Online:2022-02-25 Published:2022-03-18

摘要: 为研究对节白蜡(Fraxinus hupehensis)与“京翠”在分子水平的遗传距离和遗传一致性。利用叶绿体微卫星(Chloroplast microsatellite,cpSSR)分子标记技术,对京山地区采集到的对节白蜡和“京翠”进行亲缘关系研究,并与其他物种共同构建UPGMA聚类图。结果发现,22对cpSSR引物共扩增出了88条多态性带,根据遗传一致度构建2个样本和其他物种数据的UPGMA聚类图,10个样本聚成5个大类:第Ⅰ类包括2个样本,分别为“京翠”和豆科物种;第Ⅱ类为对节白蜡、茄科、猕猴桃科、蔷薇科和桃金娘科;龙舌兰科、禾本科和十字花科分别归属于第Ⅲ、Ⅳ和Ⅴ类。这表明对节白蜡与“京翠”之间存在一定的亲缘性,但是两者亲缘性不高。

关键词: 对节白蜡(Fraxinus hupehensis), "京翠", cpSSR, 亲缘关系, UPGMA

Abstract: In order to study the genetic distance and genetic consistency between Fraxinus hupehensis and “Jingcui” at molecular level. The cpSSR (Chloroplast microsatellite) molecular markers technology were applied to determine the genetic relationship between Fraxinus hupehensis and “Jingcui” collected in Jingshan area, then UPGMA clustering map was constructed through combining the data from other species. The results showed that 88 polymorphic bands were amplified by 22 pairs of cpSSR primers, and UPGMA clustering was constructed for 2 samples according to genetic consistency. The 10 species were clustered into 5 categories, the first category included 2 samples, namely “Jingcui” and Leguminous; the second category was composed of Fraxinus hupehensis, Solanaceae, Actinidiaceae, Rosaceae and Myrtaceae; Agaveaceae, Gramineae and Cruciferae were belong to the III, IV, and V category, separately. The results showed that there was a certain affinity between Fraxinus hupehensis and “Jingcui”, but the affinity was not high.

Key words: Fraxinus hupehensis, Jingcui, cpSSR, genetic relationship, UPGMA

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