饲料中添加不同含量的硒对黄河鲤鱼幼鱼生长性能和血液参数的影响

doi:10.14088/j.cnki.issn0439-8114.2022.04.024

摘要/Abstract

摘要： 为研究饲料中添加不同含量的硒（Se）对黄河鲤鱼（Cyprinus carpio）幼鱼生长性能和血液参数的影响,选取240尾初体重（17.21±1.73）g、初体长（11.11±0.46）cm的健康黄河鲤鱼幼鱼为研究对象,随机分为4组,每组3个重复,每个重复20尾,分别在饲料中添加0（对照组）、0.25、0.50、1.00 mg/kg的亚硒酸钠,测定黄河鲤鱼的生长性能和各项血液参数。结果表明,①饲料中添加不同含量的硒显著提高了黄河鲤鱼幼鱼的终末体重（P<0.05）,不同程度地提高了黄河鲤鱼幼鱼增重率（WGR）和特定生长率（SGR）,其中0.50 mg/kg组的WGR、SGR显著高于对照组（P<0.05）,饵料系数（FCR）显著低于对照组（P<0.05）;饲料中硒水平对黄河鲤鱼幼鱼成活率（SR）无显著影响（P>0.05）。②随着饲料中硒含量的增加,黄河鲤鱼幼鱼中红细胞数量（RBC）、血红蛋白含量（Hb）以及平均血红蛋白浓度（MCHC）有所升高;在0.50 mg/kg组Hb最大,在1.00 mg/kg组RBC和MCHC最大,均显著高于对照组（P<0.05）;平均红细胞体积（MCV）和红细胞平均血红蛋白含量（MCH）随饲料硒含量的增加而降低,于1.00 mg/kg组达最低,显著低于对照组（P<0.05）;血细胞比容（Hct）不受饲料硒含量的影响。黄河鲤鱼幼鱼饲料中硒的最适添加量为0.50 mg/kg。

关键词: 亚硒酸钠, 黄河鲤鱼（Cyprinus carpio）, 生长性能, 血液参数

Abstract: To study the effects of dietary selenium （Se） on growth performance and blood parameters of juvenile Yellow River carp, a total of 240 healthy Yellow River carp juveniles, with initial body weight of （17.21±1.73） g and initial body length of （11.11±0.46） cm, were selected as the research objects. The fishes were randomly divided into 4 groups, with 3 replicates in each group and 20 replicates in each group 0 （control group）, 0.25, 0.50 and 1.00 mg/kg sodium selenite were added to the dietary of each group, respectively. The experimental fishes were fed with basic diet for one week, and then the formal experiment was carried out for eight weeks. After the experiment, the growth performance and blood parameters of fishes were measured. The results showed that the final body weight of Yellow River carp juveniles was significantly increased by adding different contents of selenium in the dietary （P<0.05）, The weight gain rate （WGR） and specific growth rate （SGR） of Yellow River carp were increased in different degrees. The WGR and SGR of 0.50 mg/kg group were significantly higher than that of the control group （P<0.05）, the feed coefficient rate（FCR） was significantly lower than that of the control group （P<0.05）. Dietary selenium level had no significant effect on the survival rate （SR） of juvenile Yellow River carp （P>0.05）. With the increase of selenium content in dietary, the number of red blood cells （RBC）, hemoglobin content （Hb） and mean corpuscular hemoglobin concentration （MCHC） of juvenile Yellow River carp were increased. The Hb was reached maximum in 0.50 mg/kg group, but the RBC and MCHC were reached maximum in 1.00 mg/kg group, which were significantly higher than those in control group （P<0.05）. The mean corpuscular volume （MCV） and mean corpuscular hemoglobin （MCH） decreased with the increase of dietary selenium content, and reached the lowest level in 1.00 mg/kg group, which was significantly lower than that in the control group （P<0.05）. Hematocrit （Hct） was not affected by selenium content in dietary. The optimum selenium content in the dietary of Yellow River carp was 0.50 mg/kg.

Key words: sodium selenite, Yellow River carp, growth performance, blood parameters

中图分类号:

S963.73

林帅超, 白银龙, 熊建利. 饲料中添加不同含量的硒对黄河鲤鱼幼鱼生长性能和血液参数的影响[J]. 湖北农业科学, 2022, 61(4): 121-126.

LIN Shuai-chao, BAI Yin-long, XIONG Jian-li. Effects of dietary selenium on growth performance and blood parameters of juvenile Yellow River carp[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(4): 121-126.

参考文献

[1] KHAN K U, ZUBERI A, FERNANDES J B K, et al. An overview of the ongoing insights in selenium research and its role in fishnutrition and fish health[J]. Fish physiology and biochemistry, 2017, 43(6): 1689-1705.
[2] ROTRUCK J T, POPE A L, GANTHER H E, et al.Selenium: Biochemical role as a component of glutathione peroxidase[J]. Science,1973, 179(4073): 588-590.
[3] 郝甜甜,王际英,马长兴, 等. 硒对铜胁迫下大菱鲆幼鱼生长、抗氧化能力及相关基因表达的影响[J]. 水产学报, 2019, 43(11): 2304-2316.
[4] RALSTON N V,RAYMOND L J.Dietary selenium's protective effects against methylmercury toxicity[J]. Toxicology,2010,278(1): 112-123.
[5] RAJKUMAR K, SANDHYA M V S, KOGANTI S, et al. Selenium nanoparticles synthesized using Pseudomonas stutzeri (MH191156) show antiproliferative and anti-angiogenic activity against cervical cancer cells[J]. International journal of nanomedicine, 2020, 15: 4523-4540.
[6] HUANG G, LIU Z, HE L, et al.Autophagy is an important action mode for functionalized selenium nanoparticles to exhibit anti-colorectal cancer activity[J].Biomaterials science,2018,6(9): 2508-2517.
[7] FERGUSON H W, RICE D A, LYNAS J K.Clinical pathology of myodegeneration(pancreas disease)in Atlantic salmon(Salmo salar)[J].The veterinary record, 1986, 119(12): 297-299.
[8] DEFOREST D K, BRIX K V, ADAMS W J, et al.Critical review of proposed residue-based selenium toxicity thresholds for freshwater fish[J]. Human and ecological risk assessment: An international journal, 1999, 5(6): 1187-1228.
[9] 孙德文, 詹勇, 许梓荣. 硒及其在鱼类营养上的应用[J]. 饲料博览, 2003(6): 37-39.
[10] SILVA M S, KRÖCKEL S, PRABHU P A J, et al. Apparent availability of zinc, selenium and manganese as inorganic metal salts or organic forms in plant-based diets for Atlantic salmon(Salmo salar)[J]. Aquaculture, 2019, 503: 562-570.
[11] CHEN H, LI J, YAN L, et al.Subchronic effects of dietary selenium yeast and selenite on growth performance and the immune and antioxidant systems in Nile tilapia Oreochromis niloticus[J]. Fish & shellfish immunology, 2020, 97: 283-293.
[12] LIN Y H.Effects of dietary organic and inorganic selenium on the growth, selenium concentration and meat quality of juvenile grouper Epinephelus malabaricus[J]. Aquaculture, 2014, 430: 114-119.
[13] WANG L, WANG L, ZHANG D, et al.Effect of dietary selenium on postprandial protein deposition in the muscle of juvenile rainbow trout(Oncorhynchus mykiss)[J]. British journal of nutrition, 2020, 125(7): 1-30.
[14] LONGBAF DEZFOULI M, GHAEDTAHERI A, KEYVANSHOKOOH S, et al.Combined or individual effects of dietary magnesium and selenium nanoparticles on growth performance, immunity, blood biochemistry and antioxidant status of Asian seabass(Lates calcarifer)reared in freshwater[J]. Aquaculture nutrition, 2019, 25(6): 1422-1430.
[15] WANG L, XIAO J X, HUA Y, et al.Effects of dietary selenium polysaccharide on growth performance, oxidative stress and tissue selenium accumulation of juvenile black sea bream, Acanthopagrus schlegelii[J]. Aquaculture, 2019, 503: 389-395.
[16] 梁达智, 马豪勇, 杨奇慧, 等. 硒源及硒水平对斜带石斑鱼幼鱼生长性能、免疫酶活性和全鱼及脊椎骨硒含量的影响[J].动物营养学报, 2019, 31(6): 2777-2787.
[17] LIN Y H, SHIE Y Y, SHIAU S Y.Dietary copper requirements of juvenile grouper, Epinephelus malabaricus[J]. Aquaculture, 2008, 274(1): 161-165.
[18] ZHU Y, CHEN Y, LIU Y, et al.Effect of dietary selenium level on growth performance, body composition and hepatic glutathione peroxidase activities of largemouth bass Micropterus salmoide[J]. Aquaculture research, 2012, 43(11): 1660-1668.
[19] LE K T, DAO T T, FOTEDAR R, et al.Effects of variation in dietary contents of selenium and vitamin E on growth and physiological and haematological responses of yellowtail kingfish, Seriola lalandi[J]. Aquaculture international, 2014, 22(2): 435-446.
[20] ABDEL-TAWWAB M, MOUSA M A, ABBASS F E.Growth performance and physiological response of African catfish, Clarias gariepinus (B.) fed organic selenium prior to the exposure to environmental copper toxicity[J]. Aquaculture,2007,272(1-4): 335-345.
[21] LIN Y-H, SHIAU S Y.Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus[J]. Aquaculture,2005,250(1-2): 356-363.
[22] WANG Y, HAN J, LI W, et al.Effect of different selenium source on growth performances, glutathione peroxidase activities, muscle composition and selenium concentration of allogynogenetic crucian carp(Carassius auratus gibelio)[J]. Animal feed science and technology, 2007, 134(3-4): 243-251.
[23] 徐辉碧, 黄开勋. 硒的化学、生物化学及其在生命科学中的应用[M]. 武汉: 华中理工大学出版社,1994.32-34.
[24] 梁萌青, 王家林, 常青, 等. 饲料中硒的添加水平对鲈鱼生长性能及相关酶活性的影响[J]. 中国水产科学, 2006, 13(6): 1017-1022.
[25] 王安利, 王维娜, 刘存歧, 等. 饲料中硒含量对中国对虾生长及其体内含量的影响[J]. 水产学报,1994,18(3): 245-248.
[26] 谈枫. 养殖中期鲈鱼(Lateolabrax japonicus)矿物元素硒、镁的营养需求研究[D].山东青岛: 中国海洋大学,2014.
[27] 张清雯, 陈超, 邵彦翔, 等. 硒代蛋氨酸对条纹锯鮨生长性能、组织硒含量、抗氧化能力及血清生化指标的影响[J].动物营养学报, 2018, 30(11): 475-485.
[28] 金明昌. 幼鲤硒缺乏症及其机制和硒需要量研究[D].四川雅安: 四川农业大学, 2007.
[29] HILTON J W,HODSON P V, SLINGER S J.The requirement and toxicity of selenium in rainbow trout(Salmo gairdneri)[J].The journal of nutrition, 1980, 110(12): 2527-2535.
[30] ARTHUR J R, NICOL F, BECKETT G J.Hepatic iodothyronine 5′-deiodinase. The role of selenium[J].Biochemical journal, 1990, 272(2): 537-540.
[31] BURK R F.Selenium, an antioxidant nutrient[J]. Nutrition in clinical care, 2002, 5(2): 75-79.
[32] DAWOOD M A O, KOSHIO S, ZAINELDIN A I, et al. An evaluation of dietary selenium nanoparticles for red sea bream (Pagrus major) aquaculture: Growth, tissue bioaccumulation, and antioxidative responses[J].Environmental science and pollution research, 2019, 26(30): 30876-30884.
[33] 杨原志, 聂家全, 谭北平, 等. 硒源与硒水平对军曹鱼幼鱼生长性能、肝脏和血清抗氧化指标及组织硒含量的影响[J]. 动物营养学报, 2016, 28(12): 3894-3904.
[34] 胡俊茹, 王国霞, 孙育平, 等. 饲料硒含量对黄颡鱼幼鱼生长性能、抗氧化能力和脂肪代谢基因表达的影响[J].动物营养学报, 2016, 28(12): 3925-3934.
[35] ADAMS S M,BROWN A M,GOEDE R W.A quantitative health assessment index for rapid evaluation of fish condition in the field[J]. Transactions of the American fisheries society,1993,122(1):63-73.
[36] ANDERSON I G, SCHAUMULLER L F, KRAMER H J.A preliminary study on the hematology of freshwater-reared Seabass/Barramundi, Lates calcarifer[J]. Asian fisheries science, 1996, 9: 101-108.
[37] 郝小凤. 日粮中不同硒水平对大鳞副泥鳅生理生化指标及肌肉品质的影响[D]. 江苏苏州: 苏州大学, 2013.
[38] 苏传福. 草鱼幼鱼硒的营养需要研究[D]. 重庆: 西南大学, 2008.
[39] 朱春峰. 有机硒和无机硒对异育银鲫生长、生理的影响[D]. 江苏苏州: 苏州大学, 2009.
[40] RIDER S A, DAVIES S J, JHA A N, et al.Supra-nutritional dietary intake of selenite and selenium yeast in normal and stressed rainbow trout(Oncorhynchus mykiss): implications on selenium status and health responses[J]. Aquaculture, 2009, 295(3-4): 282-291.
[41] ILHAM I, SIDDIK M A B, FOTEDAR R. Effects of organic selenium supplementation on growth, accumulation, haematology and histopathology of juvenile barramundi(Lates calcarifer)fed high soybean meal diets[J]. Biological trace element research, 2016, 174(2): 436-447.
[42] BELL J G, COWEY C B, ADRON J W, et al.Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout(Salmo gairdneri)[J]. British journal of nutrition, 1985, 53(1): 149-157.