湖北农业科学 ›› 2021, Vol. 60 ›› Issue (23): 127-130.doi: 10.14088/j.cnki.issn0439-8114.2021.23.028

• 畜牧·兽医 • 上一篇    下一篇

江汉鸡群体FSHβ基因遗传多态位点分析

黄涛1, 潘爱銮1, 申杰1, 吴艳1, 蒲跃进1, 张昊1, 孙静1, 梁振华1, 杜金平1, 朱昌友2, 皮劲松1   

  1. 1.湖北省农业科学院畜牧兽医研究所/湖北省农业科技创新中心/动物胚胎与分子育种湖北省重点实验室,武汉 430064;
    2.湖北省畜牧良种场,武汉 434010
  • 收稿日期:2021-08-23 出版日期:2021-12-10 发布日期:2021-12-21
  • 通讯作者: 皮劲松,研究员,主要从事家禽育种与养殖的研究,(电话)13886171120(电子信箱)pijin-song@sina.com。
  • 作者简介:黄涛(1989-),男,河南光山人,助理研究员,博士,主要从事家禽遗传育种研究,(电话)13100655209(电子信箱)huangtao214@126.com;
  • 基金资助:
    湖北省畜禽优质增效与绿色养殖技术创新行动(2018skjcx05); 湖北省农业科学院青年基金项目(2020NKYJJ11)

Analysis on genetic polymorphisms of FSHβ gene in Jianghan chicken population

HUANG Tao1, PAN Ai-luan1, SHEN Jie1, WU Yan1, PU Yue-jin1, ZHANG Hao1, SUN Jing1, LIANG Zhen-hua1, DU Jin-ping1, ZHU Chang-you2, PI Jin-song1   

  1. 1. Institute of Animal Husbandry and Vetervinary,Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding,Wuhan 430064,China;
    2. Hubei Animal Husbandry Breeding Farm,Wuhan 434010,China
  • Received:2021-08-23 Online:2021-12-10 Published:2021-12-21

摘要: 利用基因组重测序技术对江汉鸡群体卵泡雌激素(FSH)β亚基基因序列进行全面筛选,结果表明,该群体内一共发现35个SNPs和2个indels,其中cds区1个SNP突变造成甘氨酸变成丝氨酸。结合生物信息学软件分析基因保守序列,预测M6A修饰位点、调控元件以及miRNA靶向序列,结果发现7个SNPs位于潜在的调控元件序列中,7个SNPs和1个indel位于潜在miRNA结合序列中。所筛选的上述遗传变异位点可能参与了FSHβ基因的表达调控,在江汉鸡繁殖特性中扮演一定的角色。

关键词: FSHβ;, SNP, 江汉鸡

Abstract: In this study, the genome-wide re-sequencing technology was applied in Jianghan chicken population for genetic variation identification, the results showed that, 35 single nucleotide polymorphisms (SNPs) and 2 indels in the β subunit gene of follicular estrogen (FSH) were found, one single-base mutation in the cds could cause glycine to serine. Combining with bioinformatics softwares, M6A modification site prediction, regulatory element prediction and miRNA targeting sequence prediction were further analyzed. It was found that 7 single-base mutations were located in the potential regulatory element sequence, and 7 single-base mutations and 1 indel were located in the potential miRNA binding sequence. Those genetic mutations may be involved in the regulation of FSHβ gene expression, and play a certain role in the genetic characteristics of Jianghan chicken.

Key words: FSHβ;, SNP, Jianhan chicken

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