湖北农业科学 ›› 2021, Vol. 60 ›› Issue (23): 123-126.doi: 10.14088/j.cnki.issn0439-8114.2021.23.027

• 畜牧·兽医 • 上一篇    下一篇

一例山羊链球菌与多杀性巴氏杆菌混合感染的调查分析

潘艳1,2, 贺会利1, 冯世文1, 李军1, 袁翠平1   

  1. 1.广西壮族自治区兽医研究所/广西兽医生物技术重点实验室,南宁 530001;
    2.广西农业职业技术大学,南宁 530001
  • 收稿日期:2021-01-25 出版日期:2021-12-10 发布日期:2021-12-21
  • 作者简介:潘艳(1978-),女(壮族),广西武鸣人,副研究员,博士,主要从事动物疫病防控与分子生物学研究,(电话)13878775475(电子信箱)panyan413@163.com。
  • 基金资助:
    广西重点研发计划项目(桂科AB19245010); 国家现代农业产业体系广西牛羊产业创新团队建设项目(nycytxgxcxtd-2021-09); 国家重点研发计划项目(2021YFD1100100)

An investigation and analysis on mixed infection of Streptococcus and Pasteurella multocida in goats

PAN Yan1,2, HE Hui-li1, FENG Shi-wen1, LI Jun1, YUAN Cui-ping1   

  1. 1. Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology,Nanning 530001,China;
    2. Guangxi Agricultural Vocational University,Nanning 530001,China
  • Received:2021-01-25 Online:2021-12-10 Published:2021-12-21

摘要: 对2020年8月广西某养羊场送检的1份羊病料采用细菌学和分子生物学方法进行诊断,对分离菌株进行形态学特性、生理生化、16S rDNA测序,结果鉴定为链球菌,应用PCR方法检测到多杀性巴氏杆菌阳性。对分离菌进行药敏试验,结果显示该菌对青霉素、恩诺沙星、氧氟沙星、土霉素、头孢噻呋钠高度敏感。对分离菌进行致病性试验,结果显示该菌有较强的致病性。检测结果表明该病为链球菌与多杀性巴氏杆菌混合感染引起的。

关键词: 细菌学, 链球菌(Streptococcus), 多杀性巴氏杆菌(Pasteurella multocida), 药敏试验, 致病性

Abstract: Bacteriological and molecular biological methods were used for the diagnosis of one sample of sheep from a goat farm of Guangxi in August 2020, the isolated strain by morphology, physiology, biochemistry and 16S rDNA was sequenced, and identified as Streptococcus, Pasteurella multocida positive identified by PCR amplification. Drug sensitivity analysis were perfomed on the isolated pathogenic bateria. The drug susceptibility test showed that the bacterium was highly sensitive to penicillin, enoxacin, ofloxacin, oxytetracycline, cefotifuran sodium. The pathogenicity test showed that this strain has strong pathogenicity. According to the test results, the disease is caused by the mixed infection of Streptococcus and Pasteulobacter multocida.

Key words: bacteriology, Streptococcus, Pasteurella multocida, drug sensitivity test, pathogenicity

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