羊流产嗜性衣原体蛋白-蛋白相互作用网络的构建

doi:10.14088/j.cnki.issn0439-8114.2021.22.026

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (22): 124-127.doi: 10.14088/j.cnki.issn0439-8114.2021.22.026

羊流产嗜性衣原体蛋白-蛋白相互作用网络的构建

刘威, 袁芳艳, 周丹娜, 刘泽文, 高婷, 郭锐, 杨克礼, 段正赢, 梁婉, 田永祥

湖北省农业科学院畜牧兽医研究所/农业农村部兽用药物与诊断技术湖北科学观测实验站,武汉 430064

收稿日期:2021-04-25 出版日期:2021-11-25 发布日期:2021-12-10
通讯作者: 田永祥（1965-）,男,湖北武汉人,研究员,主要从事家畜主要疫病生物制剂的研制及综合防控技术研究,（电子信箱）tyxanbit@163.com。
作者简介:刘威（1985-）,男,湖北武汉人,助理研究员,博士,主要从事动物传染病研究工作,（电子信箱）liuwei85@126.com。
基金资助:
国家重点研发计划项目（2016YFD0500906）; 国家自然科学基金项目（315020069）; 湖北省农业科技创新中心资助项目（2016-620-000-001-026）

Construction of the protein-protein interaction network of Chlamydia abortus

LIU Wei, YUAN Fang-yan, ZHOU Dan-na, LIU Ze-wen, GAO Ting, GUO Rui, YANG Ke-li, DUAN Zheng-ying, LIANG Wan, TIAN Yong-xiang

Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Hubei Science-observation Experiment Station of Veterinary Medicine and Diagnostic Technology （Ministry of Agriculture and Rural Affairs）,Wuhan 430064,China

Received:2021-04-25 Online:2021-11-25 Published:2021-12-10

摘要/Abstract

摘要： 病原菌的相互作用组学研究是揭示潜在信号转导通路和新型药物靶标的有力工具。通过同源蛋白映射的方法构建了羊流产嗜性衣原体（Chlamydia abortus）的蛋白-蛋白相互作用网络。结果表明,所构建的互作网络包含220个蛋白和1 276个相互作用关系。通过分析蛋白的互作频率,确定了在羊流产嗜性衣原体中互作频率最高的20个蛋白,主要与转录、翻译、分子伴侣等功能相关。进一步分析发现,伴侣蛋白DnaK、GroEL、DnaJ发生互作的频率较高,能与核糖体蛋白、代谢相关蛋白等多种功能蛋白发生相互作用。若DnaK、GroEL、DnaJ的功能受到抑制,将会对羊流产嗜性衣原体的正常生命活动造成较大的影响。

关键词: 羊流产嗜性衣原体（Chlamydia abortus）, 蛋白相互作用, 互作网络

Abstract: Analysis of the pathogen interactome is a powerfull approach for dissecting potential signal trasducion and new drug targets. A comprehensive protein-protein interaction network for Chlamydia abortus consisting of 220 proteins and 1 276 interaction pairs was constructed. Our analysis unraveled 20 proteins with highest interactions in the network, most of them are related to the function of transcription, translation and molecular chaperone. Further analysis showed that the chaperone proteins DnaK, GroEL and DnaJ had a high frequency of interaction, and could interact with multiple functional proteins such as ribosomal proteins and metabolism-related proteins. The results suggested that if the function of DnaK, GroEL and DnaJ were inhibited, the normal life activities of Chlamydia abortus would be greatly affected. Taken together, this study therefore provides valuable clues in exploring new signaling protein and new drug targets.

Key words: Chlamydia abortus, protein interaction, interaction network

中图分类号:

S852.67

刘威, 袁芳艳, 周丹娜, 刘泽文, 高婷, 郭锐, 杨克礼, 段正赢, 梁婉, 田永祥. 羊流产嗜性衣原体蛋白-蛋白相互作用网络的构建[J]. 湖北农业科学, 2021, 60(22): 124-127.

LIU Wei, YUAN Fang-yan, ZHOU Dan-na, LIU Ze-wen, GAO Ting, GUO Rui, YANG Ke-li, DUAN Zheng-ying, LIANG Wan, TIAN Yong-xiang. Construction of the protein-protein interaction network of Chlamydia abortus[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(22): 124-127.

参考文献

[1] SIARKOU V, LAMBROPOULOS A F, CHRISAFI S, et al.Subspecies variation in greek strains of Chlamydophila abortus[J]. Vet Microbiol, 2002,85: 145-157.
[2] SETH-SMITH H M B, BUSÓ L S, LIVINGSTONE M, et al. European Chlamydia abortus livestock isolate genomes reveal unusual stability and limited diversity, reflected in geographical signatures[J]. BMC genomics, 2017, 18(1):344.
[3] NICOLAS P, LAURE G, KARINE L, et al.Chlamydia abortus in pregnant woman with acute respiratory[J]. Distress syndrome, 2020, 26(3): 628-629.
[4] OPOTA O, BROUILLET R, GREUB G, et al.Methods for real-time PCR-based diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus infections in an opened molecular diagnostic platform[J]. Methods Mol Biol, 2017, 1616:171-181.
[5] CHEONG H C, LEE C Y Q, CHEOK Y Y, et al. Chlamydiaceae:Diseases in primary hosts and zoonosis[J]. Microorganisms, 2019, 7:146.
[6] WALDER G, MEUSBURGER H, HOTZEL H, et al.Chlamydophila abortus pelvic inflammatory disease[J]. Emerg Infect Dis, 2003, 9:1642-1644.
[7] 吴移谋. 衣原体[M]. 北京:人民卫生出版社,2012.
[8] 张志君,周继章. 流产衣原体研究进展[J]. 动物医学进展,2017,38(4):102-107.
[9] CUI T, ZHANG L, WANG Z X, et al.Uncovering new signaling proteins and potential drug targets through the interactome analysis of Mycobacterium tuberculosis[J]. BMC genomics, 2009, 10: 118.
[10] ALTSCHUL S F, MADDEN T L, SCHAFFER A A, et al.Gapped BLAST and PSI-BLAST:A new generation of protein database search programs[J]. Nucleic Acids Res,1997, 25: 3389-3402.
[11] SHANNON P, MARKIEL A, OZIER O, et al.Cytoscape:A software environment for integrated models of biomolecular interaction networks[J]. Genome Res, 2003, 13:2498-504.
[12] 杨金莹,孙爱清,刘箭,等.热激蛋白ClpB的结构和功能[J].植物生理学通讯,2006,42(2):326-330.
[13] SEGURA-PENA D, SEKULIC N, ORT S.Substrate-induced conformational changes in human UMP/CMP kinase[J]. J Biol Chem, 2004, 279(32):33882-33889.
[14] ZHAXYBAYEVA O, GOGARTEN J P. Bootstrap, Bayesian probability and maximum likelihood mapping:Exploring new tools for comparative genome analyses[J]. BMC genomics, 2002, https://doi.org/10.1186/1471-2164-3-4.

羊流产嗜性衣原体蛋白-蛋白相互作用网络的构建

Construction of the protein-protein interaction network of Chlamydia abortus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价