月季基因组SPL转录因子鉴定及表达特征分析

doi:10.14088/j.cnki.issn0439-8114.2021.21.032

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (21): 146-151.doi: 10.14088/j.cnki.issn0439-8114.2021.21.032

月季基因组SPL转录因子鉴定及表达特征分析

张宇鑫¹, 李惠¹, 张梦¹, 宋翔², 余游¹, 余马¹, 陈华¹

1.西南科技大学生命科学与工程学院,四川绵阳 621010;
2.绵阳市农畜产品质量安全检验检测中心,四川绵阳 621000

收稿日期:2021-07-21 出版日期:2021-11-10 发布日期:2021-11-24
通讯作者: 陈华（1985-）,女,副研究员,博士,主要从事生物信息学研究,（电子信箱）hchen@swust.edu.cn。
作者简介:张宇鑫（1999-）,男,辽宁辽阳人,在读本科生,研究方向为制药工程,（电话）15841988083（电子信箱）1158958441@qq.com。
基金资助:
西南科技大学博士基金项目（19ZX7117）

Genome-wide identification of the SPL gene family in Rosa chinensis and expression analysis

ZHANG Yu-xin¹, LI Hui¹, ZHANG Meng¹, SONG Xiang², YU You¹, YU Ma¹, CHEN Hua¹

1. School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;
2. Mianyang Agricultural and Animal Products Quality and Safety Inspection and Inspection Center,Mianyang 621000,Sichuan,China

Received:2021-07-21 Online:2021-11-10 Published:2021-11-24

摘要/Abstract

摘要： 研究对月季（Rosa chinensis）基因组内RcSPL家族成员进行检索,并对其进行系统发育与进化分析、基因结构分析及转录组水平的表达模式分析。结果表明,月季共含有17个SPL基因,分别命名为RcSPL1至RcSPL17。通过系统发育分析将这17个基因分为Ⅰ~Ⅷ亚组。顺势作用元件预测发现RcSPL基因上游2 000 bp含有2~13个MYB与MYC的作用位点。转录组表达分析发现,RcSPL1可能参与调控月季花粉形成,RcSPL3可能影响月季开花时间。月季RcSPL家族基因Ⅱ亚组具有最多保守基序和外显子,可能在月季花开过程行使重要的功能;它们可能与MYB和MYC相互作用共同调控花的生长发育。

关键词: 月季（Rosa chinensis）, SPL基因家族, 表达分析

Abstract: RcSPL family members were identified from the genome of Rosa chinensis, and phylogenetic and evolutionary analysis, gene structure analysis and expression pattern analysis of transcriptome level were applied for further analysis. The results showed that seventeen SPL genes named RcSPL1~RcSPL17 in Rosa chinensis were found out and they were divided into Ⅰ~Ⅷ subgroups according to the phylogenetic analysis. The original prediction of cis-acting element found that the upstream 2 000 bp of the RcSPL gene contained 2~13 MYB and MYC sites. Expression analysis found that RcSPL1 might regulate rose pollen formation, while RcSPL3 might be involved in regulating Rosa chinensis flowering time. Subgroup II carry the most conserved motifs and exons and may perform important functions during flowering. RcSPL genes could interact with MYB and MYC to regulate flower growth and development.

Key words: Rosa chinensis, SPL gene family, expression analysis

中图分类号:

S685.12
Q786

张宇鑫, 李惠, 张梦, 宋翔, 余游, 余马, 陈华. 月季基因组SPL转录因子鉴定及表达特征分析[J]. 湖北农业科学, 2021, 60(21): 146-151.

ZHANG Yu-xin, LI Hui, ZHANG Meng, SONG Xiang, YU You, YU Ma, CHEN Hua. Genome-wide identification of the SPL gene family in Rosa chinensis and expression analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 146-151.

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月季基因组SPL转录因子鉴定及表达特征分析

Genome-wide identification of the SPL gene family in Rosa chinensis and expression analysis

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