绞股蓝多糖对D-半乳糖致衰小鼠肾脏组织结构的影响

doi:10.14088/j.cnki.issn0439-8114.2021.21.022

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (21): 94-96.doi: 10.14088/j.cnki.issn0439-8114.2021.21.022

绞股蓝多糖对D-半乳糖致衰小鼠肾脏组织结构的影响

刘新勇¹, 黎木兰², 黄嘉琳¹, 王欣雨¹, 唐小川¹, 王晓丽¹

1.广西大学动物科学技术学院,南宁 530005;
2.南宁市动物疫病预防控制中心,南宁 530001

收稿日期:2020-12-07 出版日期:2021-11-10 发布日期:2021-11-24
通讯作者: 王晓丽,教授,博士,（电话）15994384384（电子信箱）xiaoliwang@gxu.edu.cn。
作者简介:刘新勇（1994-）,男,广西南宁人,在读硕士研究生,研究方向为动物组织胚胎学,（电话）13978685137（电子信箱）349478746@qq.com;共同第一作者,黎木兰（1988-）,女,兽医师,硕士,主要从事动物疫病防控的研究工作,（电话）14795647216（电子信箱）443645958@qq.com。
基金资助:
广西自然科学基金项目（2016GXNSFAA380039; 2018GXNSFAA281020）

Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose

LIU Xin-yong¹, LI Mu-lan², HUANG Jia-lin¹, WANG Xin-yu¹, TANG Xiao-chuan¹, WANG Xiao-li¹

1. College of Animal Science and Technology,Guangxi University,Nanning 530005,China;
2. Nanning Animal Disease Prevention and Control Centre,Nanning 530001,China

Received:2020-12-07 Online:2021-11-10 Published:2021-11-24

摘要/Abstract

摘要： 为探究绞股蓝多糖（Gynostemma pentaphyllum polysaccharide,GPP）对D-半乳糖（D-galactose,D-gal）致衰老小鼠肾脏组织结构的影响,采用石蜡切片、HE染色法,同时结合光学显微镜观察并对肾脏中肾小球直径和肾小囊宽度进行测量统计分析。结果发现,D-gal组肾小球明显萎缩,直径变小,肾小囊腔变大,肾小体周围肾小管管腔明显,管壁上皮细胞界限不清,模糊。GPP各剂量组均能较明显地改善肾脏的结构损伤,肾小球形态趋于正常,周围的肾小管结构明显恢复。统计分析发现,GPP各浓度组的肾小球直径均显著高于D-gal组（P<0.05）,各多糖组间肾小球直径差异均不显著。GPP 50 mg/（kg·d）组和100 mg/（kg·d）组与D-gal组相比,肾小囊宽度减小,差异显著（P<0.05）。结果表明,GPP对D-gal致衰老小鼠肾脏损伤有所恢复,结构形态趋于正常;GPP可以使肾小球直径、肾小囊宽度有所改善,说明GPP对D-gal致衰老小鼠的肾脏组织结构有一定的保护作用。

关键词: 绞股蓝多糖（Gynostemma pentaphyllum polysaccharide）, 肾脏, 衰老, D-半乳糖, 小鼠

Abstract: In order to investigate the effect of Gynostemma pentaphyllum polysaccharide （GPP） on the renal tissue structure of aging mice induced by D-galactose （D-gal）, paraffin section and HE staining were used to observe and analyze the glomerular diameter and renal capsule width in kidney. The results showed that in D-gal group, the glomeruli were significantly atrophied, the diameter of glomeruli became smaller, the lumen of renal tubules around the renal corpuscle was obvious, and the boundary of epithelial cells on the wall was unclear. GPP groups could significantly improve the structure of kidney damage, glomerular morphology tends to normal, the structure of renal tubules around the recovery. Statistical analysis showed that the glomerular diameter of GPP groups was significantly higher than that of D-gal group （P<0.05）, and there was no significant difference in glomerular diameter among the GPP groups. Compared with D-gal group, the width of renal capsule in 50 mg/（kg·d） group and 100 mg/（kg·d） group was significantly decreased （P<0.05）. It turned out that GPP could recover the kidney injury induced by D-gal, and the structural morphology tended to be normal; the diameter of glomerulus and the width of renal capsule could be improved, which indicated that GPP had a certain protective effect on the renal tissue structure of aging mice induced by D-gal.

Key words: Gynostemma pentaphyllum polysaccharide, kidney, aging, D-galactose, mice

中图分类号:

S859.4

刘新勇, 黎木兰, 黄嘉琳, 王欣雨, 唐小川, 王晓丽. 绞股蓝多糖对D-半乳糖致衰小鼠肾脏组织结构的影响[J]. 湖北农业科学, 2021, 60(21): 94-96.

LIU Xin-yong, LI Mu-lan, HUANG Jia-lin, WANG Xin-yu, TANG Xiao-chuan, WANG Xiao-li. Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 94-96.

参考文献

[1] FINKEL T, HOLBROOK N J.Oxidants, oxidative stress and the biology of ageing[J]. Nature, 2000, 408(9):239-247.
[2] PENG X, KONG B, YU H, et al.Protective effect of whey protein hydrolysates against oxidative stress in dgalactose-induced ageing rats[J]. International dairy journal, 2014, 34(1): 80-85.
[3] 魏芬芬, 王文娟, 张波. 枸杞多糖缓解小鼠体力疲劳研究[J]. 食品研究与开发, 2020, 41(6): 48-52.
[4] 龚天佐, 王世华. 当归多糖对D-半乳糖致小鼠肾脏亚急性损伤的保护作用及机制[J]. 当代医学, 2019, 25(29): 47-49.
[5] YU S S, YU J, DONG X D, et al.Structural characteristics and anti-tumor/-oxidant activity in vitro of an acidic polysaccharide from Gynostemma pentaphyllum[J]. International journal of biological macromolecules,2020,161(10):721-728.
[6] REN D Y,ZHAO Y,ZHENG Q,et al.Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells[J]. Food and function,2019, 10(4): 2186-2197.
[7] ANDERSON S,BRENNER B M,李金爱,等. 肾脏的衰老及其治疗[J]. 国外医学(老年医学分册),1989,10(3):114-115.
[8] 王佳贺. 前言—衰老相关机制的研究进展[J]. 实用老年医学, 2018, 32(12):1101-1102.
[9] 李典耕, 孙雪峰, 陈香美. 肾脏衰老机制的探讨[J]. 中华老年多器官疾病杂志, 2016, 15(4): 302-306.
[10] SARKAR D, FISHER P B.Molecular mechanisms of aging-associated inflammation[J]. Cancer letters,2006,236(1):13-23.
[11] COSTELLO W R, RYFF C D, COE C L.Aging and low-grade inflammation reduce renal function in middle-aged and older adults in Japan and the USA[J]. Age, 2015, 37(4): 75-84.
[12] 王珂欣, 高丽, 周玉枝,等. 衰老过程中炎症相关的信号传导通路研究进展[J]. 中国药理学与毒理学杂志, 2016, 30(10): 1108-1113.
[13] 刘晓梅, 陈尚. 枸杞多糖对衰老小鼠肾小球形态学指标的影响[J]. 中国老年学杂志, 2009, 29(18):2354-2355.
[14] 丁秋英. 金福菇多糖抗衰老作用研究[D]. 合肥:安徽大学,2016.
[15] 刘胜阳, 栗霄立. 灵芝多糖通过上调SIRT1的表达缓解肾脏衰老[J]. 临床和实验医学杂志, 2018, 17(6): 568-572.
[16] 蔡曦光, 许爱霞, 葛斌, 等. 菟丝子多糖抑制衰老小鼠模型中氧自由基域的作用[J]. 第三军医大学学报,2005,27(13): 1326-1328.
[17] 蒲秀瑛, 李海兵, 李晓玥, 等. 归芪多糖对衰老大鼠肾组织氧化损伤作用的研究[J]. 中医药学报, 2017, 45(2):15-18.
[18] 李丹丹, 刘佳佳, 刘军军, 等. 黄芪多糖联合二甲双胍对衰老2型糖尿病小鼠肾组织糖原代谢及超微结构的影响[J]. 时珍国医国药, 2019, 30(4):827-829.
[19] 傅紫琴, 蔡宝昌, 余宗亮, 等. 山茱萸炮制前后多糖成分对肾阴虚模型小鼠的抗衰老作用[J]. 中药新药与临床药理, 2007, 18(6):437-439.
[20] 金鑫. 绞股蓝多糖对小鼠腹腔巨噬细胞影响的研究[D]. 南宁:广西大学, 2018.
[21] WANG Z C, WANG Z, HUANG W H, et al.Antioxidant and anti-inflammatory activities of an anti-diabetic polysaccharide extracted from Gynostemma pentaphyllum herb[J]. International journal of biological macromolecules, 2020, 145(4):484-491.

绞股蓝多糖对D-半乳糖致衰小鼠肾脏组织结构的影响

Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose

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