湖北农业科学 ›› 2021, Vol. 60 ›› Issue (21): 73-75.doi: 10.14088/j.cnki.issn0439-8114.2021.21.017

• 园艺·特产 • 上一篇    下一篇

白术腋芽离体快繁技术初探

李红英1,2, 陈菲菲1,2, 殷红清1,2, 覃大吉1,2, 孙举志1,2, 杨永康1,2, 向极钎1,2, 李亚杰1   

  1. 1.恩施土家族苗族自治州农业科学院,湖北 恩施 445000;
    2.湖北省富硒产业技术研究院,湖北 恩施 445000
  • 收稿日期:2021-10-02 出版日期:2021-11-10 发布日期:2021-11-24
  • 通讯作者: 向极钎(1967-),男,正高职农艺师,主要从事生物技术与植物提取研究,(电话)13972400610(电子信箱)2416861638@qq.com。
  • 作者简介:李红英(1982-),女,湖北利川人,助理研究员,硕士,主要从事植物组织培养与食用菌富硒培养工作,(电话)13872740626(电子信箱)522070555@qq.com。
  • 基金资助:
    恩施州科技计划项目(D20180021)

In vitro rapid propagation of axillary buds of Atractylodes macrocephala

LI Hong-ying1,2, CHEN Fei-fei1,2, YIN Hong-qing1,2, QIN Da-ji1,2, SUN Ju-zhi1,2, YANG Yong-kang1,2, XIANG Ji-qian1,2, LI Ya-jie1   

  1. 1. Enshi Tujia & Miao Autonomous Prefecture Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
    2. Hubei Selenium Industry Technology Research Institute,Enshi 445000,Hubei,China
  • Received:2021-10-02 Online:2021-11-10 Published:2021-11-24

摘要: 以白术(Atractylodes macrocephala Koidz.)的腋芽、茎尖为外植体,添加不同浓度的6-BA、NAA等生长激素到MS、1/2 MS培养基中,对丛生芽进行分化诱导液体培养和生长培养;添加不同浓度的NAA、IBA到1/4 MS培养基中进行液体生根及壮苗培养,对驯化后的白术试管苗炼苗移栽。结果表明,诱导分化液体培养基为1/2 MS+1.5 mg/L 6-BA+0.4 mg/L NAA+0.3 mg/L IBA+0.6 mg/L GA3,光照时间18 h/d,光照度1 800 lx,丛生芽分化诱导形成率为97.3%;最高增值倍数为16;丛生芽生根诱导液体培养基为1/4 MS+0.4 mg/L NAA+0.2 mg/L IBA,光照18 h/d,光照度1 900 lx,生根率高达98.9%,平均生根数8.5条/芽;炼苗成活率95%以上。移栽苗圃成活率为94%。

关键词: 白术(Atractylodes macrocephala Koidz.), 腋芽, 液体培养, 丛生芽分化诱导培养

Abstract: The axillary buds and shoot tips of Atractylodes Macrocephala Koidz were used as explants, and growth hormone (6-BA, NAA, etc.) with different concentrations were added to MS and 1/2 MS medium to induce differentiation and growth of the multiple shoots. Different concentrations of NAA and IBA were added to 1/4 MS medium for liquid rooting and strong seedling culture, and the cultured tube seedlings of Atractylodes macrocephala were transplanted. The results showed that the induction rate of tufted buds was 97.3% on the medium of 1/2 MS+1.5 mg/L 6-BA+0.4 mg/L NAA+0.3 mg/L IBA+0.6 mg/L GA3 under 18 h/d and illuminance 1 800 lx. The highest value-added multiple is 16; Medium:1/4 MS+0.4 mg/L NAA+0.2 mg/L IBA, illumination 18 h/d, illuminance 1 900 lx, rooting rate up to 98.9%, the average number of rooting 8.5 per shoot; The survival rate of seedlings was more than 95%. The survival rate of transplanted nursery was 94%.

Key words: Atractylodes macrocephala, axillary bud, liquid culture, cluster bud differentiation induction culture

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