湖北农业科学 ›› 2021, Vol. 60 ›› Issue (18): 82-85.doi: 10.14088/j.cnki.issn0439-8114.2021.18.016

• 植物保护 • 上一篇    下一篇

辽宁省玉竹根腐病病原鉴定及生物学特性研究

李晓丽, 孙文松, 刘莹, 李玲, 张天静   

  1. 辽宁省经济作物研究所,辽宁 辽阳 111000
  • 收稿日期:2020-10-15 出版日期:2021-09-25 发布日期:2021-10-11
  • 通讯作者: 孙文松(1973-),男,研究员,硕士,主要从事中药材种质收集保护、品种选育及标准化栽培研究,(电子信箱)sunwensong12@126.com。
  • 作者简介:李晓丽(1979-),女,辽宁康平人,助理研究员,硕士,主要从事中药材标准化栽培技术及品种选育研究,(电话)15941988447(电子信箱)1224895821@qq.com。
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系资助项目; 辽宁省农业科学院学科建设项目(2019DD144221); 辽宁省农业科学院重大科研成果培育项目(2020CGPY0403)

Biological characterization and pathogen identification of Polygonatum odoratum root rot in Liaoning province

LI Xiao-li, SUN Wen-song, LIU Ying, LI Ling, ZHANG Tian-jing   

  1. Liaoning Research Institute of Cash Crops,Liaoyang 111000,Liaoning,China
  • Received:2020-10-15 Online:2021-09-25 Published:2021-10-11

摘要: 为鉴定辽宁省玉竹[PoLygonatum ordoratum(Mill.)Druce]根腐病病原菌的种类及其生物学特性,对玉竹根腐病病原菌进行了分离纯化培养,通过致病性测定,结合病菌形态学观察和延长因子(EF1-腐)序列分析,确定病原菌种类,并对病原菌的生物学特性进行了系统研究。结果表明,该病原菌为尖孢镰孢菌(Fusarium oxysporum),生长最适宜的培养基为马铃薯葡萄糖培养基,碳源为甘露醇,氮源为硝酸钾,温度为25~30 ℃,pH为5~7,光照条件为完全黑暗。

关键词: 玉竹[PoLygonatum ordoratum(Mill.)Druce], 根腐病, 尖孢镰孢菌(Fusarium oxysporum), 生物学特性

Abstract: To identify the pathogen of Polygonatum odoratum root rot and clarify its biological characteristics in Liaoning province, which the pathogenic strains were isolated, purified and cultured. Based on pathogenicity tests, combining with morphological observation and extension factor (EF1-α) sequence analysis, the kinds of pathogenic strains were clarified and the biological characteristics of pathogen were systematically studied. The results indicated that the pathogen was identified as Fusarium oxysporum, which the optimum medium for pathogen growth was potato dextrose agar (PDA), the carbon source and nitrogen source was mannitol and potassium nitrate, respectively. The temperature was 25~30 ℃, pH was 5~7, and the light condition was completely dark.

Key words: Polygonatum odoratum, root rot, Fusarium oxysporum, biological characteristics

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