湖北农业科学 ›› 2021, Vol. 60 ›› Issue (18): 77-81.doi: 10.14088/j.cnki.issn0439-8114.2021.18.015

• 植物保护 • 上一篇    下一篇

台湾相思甲醇提取物抑菌活性研究

庞皓元   

  1. 广西壮族自治区食品药品检验所,南宁 530021
  • 收稿日期:2021-05-25 出版日期:2021-09-25 发布日期:2021-10-11
  • 作者简介:庞皓元(1979-),男,广西南宁人,中级技师,硕士,主要从事病理及病理生理学研究,(电话)18077798510(电子信箱)674632459@qq.com。
  • 基金资助:
    广西壮瑶药工程技术研究中心资助项目(ZYMCET201904)

Antimicrobial activity of methanol extract of Acacia confusa

PANG Hao-yuan   

  1. Guangxi Zhuang Autonomous Region Institute for Food and Drug Control,Nanning 530021,China
  • Received:2021-05-25 Online:2021-09-25 Published:2021-10-11

摘要: 研究台湾相思(Acacia confusa Merr.)甲醇提取物及不同提取部位抑菌活性。采用超声波辅助提取法对台湾相思成分进行提取,用菌丝生长速率法测定提取物对11种植物病原真菌的抑菌活性,用液体培养基稀释法和滤纸片法测定提取物对14种动物病原细菌的抑菌活性;采用液-液萃取法对抑菌活性成分进行初步分离,测定各萃取部位的抑菌活性。结果表明,台湾相思甲醇提取物具有广谱抑菌活性,在10.0 g/L浓度下,对8种植物病原真菌和9种动物病原细菌有抑制活性。其中对甘蓝黑斑病菌(Alternaria oleracea)、玉米大斑病菌(Exserohilum turcicum)和甘蔗凤梨病菌(Ceratocystis paradoxa)的抑菌率均达89.55%以上,对蜡样芽孢杆菌(Bacillus cereus)、枯草芽孢杆菌(Bacillus subtilis)和痢疾志贺氏菌(Shigella dysenteriae)的最低抑制浓度为1.0 g/L;台湾相思对植物病原真菌和动物病原细菌均有广谱抑制作用,活性部位主要在正丁醇、乙酸乙酯部位。

关键词: 台湾相思(Acacia confusa Merr.), 甲醇提取物, 抑菌活性, 活性部位

Abstract: The antimicrobial activity of the methanol extract of Acacia confusa and its different extraction parts were studied. Ultrasonic extraction was used to extract the components of Acacia confusa, the antimicrobial activity of the extracts against 11 phytopathogenic fungi was determined by the mycelial growth rate method, and the extracts against 14 species of animals pathogenic bacteria were determined by the liquid medium dilution method and the filter paper method. The antimicrobial activities of the extracts were determined by liquid-liquid extraction to preliminarily separate the antimicrobial active ingredients and determine the antibacterial activity of each extract. The results showed that the methanol extract of Acacia confusa has broad-spectrum antimicrobial activity. At a concentration of 10.0 g/L, it had inhibitory activity against 8 kinds of plant pathogenic fungi and 9 kinds of animal pathogenic bacteria. Among them, the inhibitory rate against Brassica oleracea, Exserohilum turcicum and Ceratocystis paradoxa reached more than 89.55%, and the minimum inhibitory concentration against Bacillus cereus, Bacillus subtilis and Shigella dysenteriae was 1.0 g/L; Acacia confusa had inhibitory effects on plant pathogenic fungi and animal pathogenic bacteria, and the action parts were n-butanol and ethyl acetate.

Key words: Acacia confuse Merr., methanol extract, antimicrobial activity, active part

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