丁酸梭菌CB1制剂对肉鸡生产性能、肠道形态和菌群的影响

doi:10.14088/j.cnki.issn0439-8114.2021.17.020

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (17): 93-97.doi: 10.14088/j.cnki.issn0439-8114.2021.17.020

丁酸梭菌CB1制剂对肉鸡生产性能、肠道形态和菌群的影响

何菊¹, 李筱雯², 崔卫涛², 郭杰¹, 王纯¹, 肖运才¹, 李自力¹, 周祖涛¹

1.华中农业大学动物医学院/湖北省预防兽医学重点实验室,武汉 430070;
2.湖北华大瑞尔科技有限公司/生物发酵饲料湖北省工程研究中心,武汉 430070

收稿日期:2020-12-07 出版日期:2021-09-10 发布日期:2021-09-30
通讯作者: 周祖涛（1980-）,男,副教授,博士,主要从事兽医微生物与免疫学的研究工作,（电话）13114373570（电子信箱）ztzhou@mail.hzau.edu.cn。
作者简介:何菊（1990-）,女,贵州六盘水人,硕士,主要从事兽医微生物与免疫学方向研究,（电话）15185048696（电子信箱）3354797775@qq.com
基金资助:
广西创新驱动发展专项（桂科AA18118051）

Effects of Clostridium butyricum CB1 preparations on production performance,intestinal morphology and microflora of broilers

HE Ju¹, LI Xiao-wen², CUI Wei-tao², GUO Jie¹, WANG Chun¹, XIAO Yun-cai¹, LI Zi-li¹, ZHOU Zu-tao¹

1. College of Veterinary Medicine,Huazhong Agricultural University/Hubei Key Laboratory of Preventive Veterinary Medicine,Wuhan 430070,China;
2. Hubei Huada Real Science &Technology Co. ,Ltd. /Hubei Engineering Research Center of Biofermentation Feed,Wuhan 430070,China;

Received:2020-12-07 Online:2021-09-10 Published:2021-09-30

摘要/Abstract

摘要： 本研究旨在探讨丁酸梭菌CB1及其复合菌制剂对肉鸡生产性能、肠道形态和菌群结构的影响。选取1日龄Cobb500肉鸡15 000只,随机分为5组,每组3个重复,每个重复1 000只,分别饲喂：A组为基础日粮、B组含有50 g/t金霉素的日粮、C1、C2和D组分别在基础日粮中添加100 g/t丁酸梭菌CB1制剂、200 g/t丁酸梭菌CB1制剂、200 g/t丁酸梭菌CB1制剂复合菌制剂,试验期为42 d。试验结果表明,C1、C2和D组1~21 d平均日增重高于B组（P<0.05或P<0.01）,D组1~42 d平均日增重与B组相当（P>0.05）,C2组和D组的料肉比、死亡率均明显低于A组和B组,D组盲肠绒毛高度和VH/CD值最高。在日粮中添加丁酸梭菌CB1制剂对肉鸡回肠、盲肠乳酸杆菌和双歧杆菌的增殖有显著促进作用。由此可见,丁酸梭菌CB1复合菌制剂具有与金霉素相当的促生长效果,在日粮中添加丁酸梭菌CB1制剂可以显著提高肠道绒毛高度和VH/CD值,有效促进肉鸡的生长性能,促进肠道形态发育,改善肠道菌群。

关键词: 丁酸梭菌CB1, 肉鸡, 生产性能, 肠道形态和菌群, 抗生素替代

Abstract: This study was designed to investigate the effects of Clostridium butyricum CB1 preparations and composite probiotics added to the diets on broiler production performance, intestinal morphology and structure of microbial community. A total of 15 000 one-day old of Cobb500 broiler chickens were randomly divided into 5 groups, with 3 replicates in each group and 1 000 chickens in each replicate, they were fed with：group A （basal diet）, group B （basal diet supplemented with 50 g/t chlortetracycline）, group C1, C2 and D were basal diets supplemented with 100 g/t C. butyricum CB1 preparation, 200 g/t C. butyricum CB1 preparation and 200 g/t C. butyricum CB1 preparation and Bacillus subtilis preparation, 42 days trial period. The test results showed that broilers in antibiotic （chlortetracycline） group B and group C1, C2 and D fed with C. butyricum CB1 preparation could increase the average body weight in different levels. The average daily weight gain of groups C1, C2 and D from 1 to 21 days were higher than that in antibiotic group B （P<0.05 or P<0.01）, and the average daily gain of group D from 1 to 42 days was similar to that of group B （P>0.05）. During the test period, the feed conversion ratio and death rate of group C2 and group D was obviously below basal diet group A and antibiotic group B, and of these the height of cecum villus and VH/CD ratio of group D was the highest. Adding C. butyricum CB1 to the diet could significantly promote the proliferation of Lactobacillus and Bifidobacterium in ileum and cecum of broilers. It can be seen that the C. butyricum CB1 complex bacteria preparation has a growth-promoting effect equivalent to that of chlortetracycline. C. butyricum CB1 preparation added in diets can significantly increase the height of intestinal villi and the VH/CD value, effectively promote the growth performance of broilers, enhance immune function, promote the development of intestinal morphology, and improve intestinal flora.

Key words: Clostridium butyricum CB1, broiler, production performance, intestinal morphology and flora, replacing antibiotics

中图分类号:

S816.7
S831

何菊, 李筱雯, 崔卫涛, 郭杰, 王纯, 肖运才, 李自力, 周祖涛. 丁酸梭菌CB1制剂对肉鸡生产性能、肠道形态和菌群的影响[J]. 湖北农业科学, 2021, 60(17): 93-97.

HE Ju, LI Xiao-wen, CUI Wei-tao, GUO Jie, WANG Chun, XIAO Yun-cai, LI Zi-li, ZHOU Zu-tao. Effects of Clostridium butyricum CB1 preparations on production performance,intestinal morphology and microflora of broilers[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(17): 93-97.

参考文献

[1] 中华人民共和国农业部公告第1231号(批准丁酸梭菌和地衣芽孢杆菌为新饲料添加剂)[J].中国饲料添加剂,2010(4):44.
[2] 刘亭婷,滑静,王晓霞,等.丁酸梭菌对蛋用仔公鸡肠道菌群、形态结构及黏膜免疫相关细胞的影响[J].动物营养学报,2012,24(11):2210-2221.
[3] YANG C M, CAO G T, FERKET P R, et al.Effects of probiotic, Clostridium Butyricum, on growth performance, immune function, and cecal microflora in broiler chickens[J]. Poultry science, 2012, 91(9): 2121-9.
[4] 苗晓微. 合生素对肉鸡生产性能的研究[D]. 吉林延吉:延边大学,2006.
[5] 郝永任. 新型动物微生态制剂—复合酪酸菌制剂的研制[D].济南:山东师范大学,2002.
[6] WANG R F, CAO W W, CERNIGLIA C E.PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples[J]. Applied & environmental microbiology, 1996, 62(4): 1242-1247.
[7] WALTER J, HERTEL C, TANNOCK G W, et al.Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis[J]. Applied & environmental microbiology, 2001, 67(6): 2578-2585.
[8] LANGENDUK P S, SCHUT F, JANSEN G J, et al.Quantitative fluorescence in situ hybridization of Bifidobacterium Spp. With genus-specific 16S rRNA-targeted probes and its application in fecal samples[J]. Applied & environmental microbiology, 1995, 61(8): 3069-3075.
[9] AMIT-ROMACH E, SKLAN D, UNI Z.Microflora ecology of the chicken intestine using 16S ribosomal DNA primers[J]. Poultry science, 2004, 83(7): 1093-1098.
[10] 张敬平,凌霞,陈悦,等.PCR检测沙门菌hilA基因的方法研究[J].中国卫生检验杂志,2008,18(12):2608-2609.
[11] 杜云平,周庆丰,郑泽铭,等.丁酸梭菌对肉鸡生产性能的影响[J].饲料广角,2009(22):34-35.
[12] 赵旭. 丁酸梭菌对肉鸡脂肪代谢的影响及其机理研究[D].北京:中国农业大学,2014.
[13] 龚琪,曾娟娟,石德时,等.益生性屎肠球菌HDRsEf1对雏鸡盲肠菌群发育的影响[J].华中农业大学学报,2015,34(1):78-82.
[14] 刘来亭,田鹏飞,杜灵广,等.酪酸菌对肉鸡生产性能和死淘率及小肠形态结构的影响[J].河南农业科学,2009(7): 130-131,134.
[15] 余成瑶,刘克琳,周毅.鸡微生物饲料添加剂饲喂肉用仔鸡扫描电镜下小肠粘膜的研究[J].四川农业大学学报,1994(S1):585-587,572.
[16] GAO Q, QI L, WU T, et al.Ability of Clostridium butyricum to inhibit Escherichia coli-induced apoptosis in chicken embryo intestinal cells[J]. Veterinary microbiology,2012,160(3):395-402.
[17] KONG Q, HE G Q, JIA J L, et al.Oral administration of Clostridium butyricum for modulating gastrointest microflora in mice[J]. Current microbiology,2011,62(2):512-517.

丁酸梭菌CB1制剂对肉鸡生产性能、肠道形态和菌群的影响

Effects of Clostridium butyricum CB1 preparations on production performance,intestinal morphology and microflora of broilers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

编辑推荐

Metrics

本文评价

[1]	王丹, 崔卫涛, 王纯, 况世昌, 许青荣, 肖运才, 李自力, 周祖涛. 发酵中药制剂对肉鸡生产性能、免疫功能、肠道形态及盲肠菌群影响的研究[J]. 湖北农业科学, 2021, 60(24): 164-168.
[2]	申杰, 黄涛, 潘爱銮, 吴艳, 皮劲松, 梁振华, 孙静, 张昊, 蒲跃进, 谢子晗. 江汉鸡A系鸡冠直立/倒伏性状对生产性能的影响[J]. 湖北农业科学, 2021, 60(21): 91-93.
[3]	申杰, 黄涛, 吴艳, 皮劲松, 潘爱銮, 梁振华, 孙静, 张昊, 蒲跃进. 江汉鸡与隐性白羽肉鸡及其正反交后代体尺和屠宰性状比较研究[J]. 湖北农业科学, 2020, 59(23): 122-125.
[4]	李振军, 吴涵, 杨烨. EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响[J]. 湖北农业科学, 2020, 59(23): 130-133.
[5]	王宏博, 李维红, 杨晓玲, 高雅琴, 席斌. 青贮甜高粱替代青贮玉米对羔羊生产性能和养分消化代谢的影响[J]. 湖北农业科学, 2020, 59(16): 112-114.
[6]	顾云锋, 周岩民, 陶正国, 程宗佳, 温超. 辣椒碱对蛋鸡生产性能、蛋品质以及抗氧化能力的影响[J]. 湖北农业科学, 2020, 59(12): 124-128.
[7]	李家凯, 郭康康, 刘丽娜, 温国元, 罗青平, 李自力, 董世山, 邵华斌, 赵宗正. 肉鸡与蛋鸡H5、H7、H9亚型禽流感病毒抗体水平调查[J]. 湖北农业科学, 2020, 59(11): 104-106.
[8]	周光元, 吴磊, 张依裕, 覃媛钰, 蒋惠梅, 周华, 杨远清, 张义玲. 黔画乌鸡种质资源调查[J]. 湖北农业科学, 2019, 58(6): 106-109.
[9]	潘爱銮, 申杰, 杨林, 蒲跃进, 吴艳, 皮劲松, 王德才, 孙静, 梁振华, 张昊, 杜金平. 商品肉鸡1234精准养殖工艺研究[J]. 湖北农业科学, 2018, 57(24): 116-119.