湖北农业科学 ›› 2021, Vol. 60 ›› Issue (13): 138-142.doi: 10.14088/j.cnki.issn0439-8114.2021.13.028

• 生物工程 • 上一篇    下一篇

不同激素对甘草带芽茎段诱导丛生芽的影响

付丽, 张东向, 刘安奇, 吕晴, 李伟   

  1. 齐齐哈尔大学生命科学与农林学院/抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江 齐齐哈尔 161006
  • 收稿日期:2020-10-27 出版日期:2021-07-10 发布日期:2021-07-26
  • 通讯作者: 张东向(1963-),男,辽宁义县人,教授,硕士,主要从事植物生理学与植物生物技术研究, (电子信箱)zhangdx1019@163.com。
  • 作者简介:付 丽(1996-),女,黑龙江宝清人,在读硕士研究生,研究方向为中草药植物组织培养,(电话)18814668148(电子信箱) 895475707@qq.com。
  • 基金资助:
    黑龙江省省属高等学校基本科研业务费科研项目(YSTSXK201887); 齐齐哈尔市科学技术计划项目(SFGG-201575); 齐齐哈尔大学研究生创新科研项目(YJSCX2019044)

Effects of different hormones on induction of clustered buds from budded stems of Glycyrrhiza uralensis Fisch

FU Li, ZHANG Dong-xiang, LIU An-qi, LYU Qing, LI Wei   

  1. College of Life Sciences and Agriculture and Forestry,Qiqihar University/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar 161006,Heilongjiang,China
  • Received:2020-10-27 Online:2021-07-10 Published:2021-07-26

摘要: 采用单因素变量和不同激素组合设计,观察不同激素配比对甘草(Glycurrihiza uralensis Fisch.)带芽茎段诱导丛生芽的影响。结果表明,诱导丛生芽的最佳激素组合为MS+0.5 mg/L 6-BA+0.5 mg/L KT,其丛生芽的平均增值倍数达到(5.00±1.61)倍。适宜的激素配比有利于甘草带芽茎段诱导丛生芽,为甘草快速繁殖技术的应用提供理论和技术依据。

关键词: 甘草(Glycurrihiza uralensis Fisch.), 带芽茎段, 丛生芽, 激素

Abstract: Single factor variable and different hormone combination design were used to observe the effect of different hormone ratio on the induction of clustered buds from the bud stem of Glycyrrhiza uralensis Fisch. The results showed that the best hormone combination for inducing cluster buds was MS+0.5 mg/L 6-BA+0.5 mg/L KT, and the average multiplication factor of cluster buds reached 5 times. Appropriate hormone ratio is beneficial to the induction of clustered buds from the budded stems of Glycyrrhiza uralensis Fisch, and provides theoretical and technical basis for the application of rapid propagation technology of Glycyrrhiza uralensis Fisch.

Key words: Glycurrihiza uralensis Fisch., budded stems, cluster buds, hormone

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