湖北农业科学 ›› 2021, Vol. 60 ›› Issue (11): 118-120.doi: 10.14088/j.cnki.issn0439-8114.2021.11.025

• 检测分析 • 上一篇    下一篇

鄂种植基地雷公藤中有机氯农药残留量测定

肖安菊1, 龚达林2, 刘三波2, 喻昕1   

  1. 1.湖北理工学院医学院,湖北 黄石 435003;
    2.华润三九(黄石)药业有限公司,湖北 黄石 435003
  • 收稿日期:2020-09-01 出版日期:2021-06-10 发布日期:2021-07-15
  • 通讯作者: 喻 昕(1969-),男,教授,博士,主要从事生化或生物药物分离研究,(电子信箱)yuxinwjh@163.com。
  • 作者简介:肖安菊(1965-),女,湖北麻城人,教授,主要从事中药学及中药材质量控制研究,(电话)13995973893(电子信箱)1157592750@qq.com。
  • 基金资助:
    湖北省卫生健康委中医药科研项目(ZY2019M070)

Determination of organochlorine pesticides in Tripterygium wilfordii from planting base of Hubei

XIAO An-ju1, GONG Da-lin2, LIU San-bo2, YU Xin1   

  1. 1. College of Medicine,Hubei Polytechnic University,Huangshi 435003,Hubei,China;
    2. China Resources Sanjiu (Huangshi) Pharmaceutical Co. Ltd.,Huangshi 435003,Hubei,China
  • Received:2020-09-01 Online:2021-06-10 Published:2021-07-15

摘要: 采用超声-回流方法提取,磺化-硅胶柱纯化,气相色谱法(GC)测定雷公藤(Tripterygium wilfordii)根中9种有机氯农药的残留含量。结果表明,2个种源3个繁殖部位繁殖生长5年的雷公藤(TJ、TG、TZ、YJ、YG、YZ)中农药总滴滴涕残留量分别为0.004 5、0.001 4、0.000 8、 0.001 2、0.003 6、0.003 3 μg/g,总六六六残留量分别为0.027 6、0.061 4、0.056 3、0.043 2、0.048 7、0.032 7 μg/g。五氯硝基苯残留量分别为0.001 9、0.001 8、0.001 5、0.001 6、0.001 6、0.001 9 μg/g,均小于《中国药典》2015版第一部规定的中草药有机氯残留标准。研究使用气相色谱法测定5年生雷公藤中农药含量,为雷公藤质量保证提供了科学依据,就农药残留而言,湖北阳新种植基地适合种植雷公藤。

关键词: 雷公藤(Tripterygium wilfordii), 六六六, DDT, 五氯硝基苯, 气相色谱法

Abstract: Organochlorine pesticides in the roots of Tripterygium wilfordii were extracted by ultrasonic-reflux method,purified by sulfonation-silica gel column, and determined by gas chromatography. The results showed that this batch comes from six new species of Tripterygium wilfordii (TJ,TG,TZ,YJ,YG,YZ) planted in Yangxin, Hubei province. The pesticide DDT residues in their roots are 0.004 5,0.001 4,0.000 8,0.001 2,0.003 6,0.003 3 μg/g respectively; the pesticide BHC residues in their roots are 0.027 6,0.061 4,0.056 3,0.043 2,0.048 7,0.032 7 μg/g respectively; the pesticide PCNB residues in their roots are 0.001 9,0.001 8,0.001 5,0.001 6,0.001 6,0.001 9 μg/g respectively. All of which are less than the organochlorine residue standard for Chinese herbal medicine stipulated in the first part of Chinese pharmacopoeia 2015 edition. In this study, the pesticide content of Tripterygium wilfordii was determined by gas chromatography, and the results provides a scientific basis for the quality assurance of Tripterygium wilfordii. In terms of pesticide residue, the new planting base in Yangxin, Hubei province was suitable for planting of Tripterygium wilfordii.

Key words: Tripterygium wilfordii, BHC, DDT, PCNB, GC

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