湖北农业科学 ›› 2021, Vol. 60 ›› Issue (11): 115-117.doi: 10.14088/j.cnki.issn0439-8114.2021.11.024

• 检测分析 • 上一篇    下一篇

顶空固相微萃取气质联用法分析羊耳菊花的挥发性成分

许培增1, 刘明娟1, 罗贞贵1, 曾雅静1, 蔡伟1,2,3   

  1. 1.湖南医药学院,湖南 怀化 418000;
    2. 湖南医药学院新型抗体药物及其智能运输系统湖南省重点实验室,湖南 怀化 418000;
    3.湖南医药学院侗医药研究湖南省重点实验室,湖南 怀化 418000
  • 收稿日期:2020-08-31 出版日期:2021-06-10 发布日期:2021-07-15
  • 通讯作者: 蔡 伟(1985-),男,湖南常德人,副教授,博士,主要从事药物分析研究,(电子信箱) 20120941161@bucm.edu.cn。
  • 作者简介:许培增(2000-),女,福建泉州人,在读本科生,研究方向为中药学,(电话)15985931905(电子信箱)644303767@qq.com。
  • 基金资助:
    国家自然科学基金项目(81603393); 湖南省自然科学基金项目(2018JJ3376)

Volatile constituents in the flowers of Inula cappa based on HS-SPME-GC-MS

XU Pei-zeng1, LIU Ming-juan1, LUO Zhen-gui1, ZENG Ya-jing1, CAI Wei1,2,3   

  1. 1. Hunan University of Medicine,Huaihua 418000,Hunan,China;
    2. Hunan Province Key Laboratory for Antibody-based Drug and Intelligent Delivery System,Hunan University of Medicine,Huaihua 418000,Hunan,China;
    3. Hunan Provincial Key Laboratory of Dong Medicine,Hunan University of Medicine,Huaihua 418000,Hunan,China
  • Received:2020-08-31 Online:2021-06-10 Published:2021-07-15

摘要: 利用顶空固相微萃取(HS-SPME)结合气相色谱质谱联用技术(GC-MS) 分析羊耳菊(Inula cappa)花的挥发性化学成分,并采用归一化法计算各成分的相对含量。结果表明,从羊耳菊花中共检测鉴定出50种化合物,占总挥发性成分的86.74%。其主要成分为百里酚(19.70%)、(-)-β-石竹烯(12.19%)、香芹酚(8.13%)和异丁百里香酯(8.13%)。该方法具有操作简便、用量少、灵敏度高且快速等优点,研究结果为其药效物质基础研究和质量控制研究奠定基础。

关键词: 羊耳菊(Inula cappa)花, 顶空固相微萃取, 气相色谱-质谱联用, 挥发性成分, 保留指数

Abstract: The volatile components in the flowers of Inula cappa were analyzed by headspace solid phase micro-extration (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that 50 compounds were identified, accounting for 86.74% of the total volatile components.Its main components include thymol (19.70%), (-)-β-caryophyllene (12.19%), carvacrol (8.13%) and thymyl isobutyrate (8.13%). This method can quickly obtain its components, is advantageous to volatile compounds, can be used for the control of Inula cappa. The method has the advantages of simple operation,low dosage,high sensitivity and high speed.The results of this study lay a foundation for the study of pharmacodynamics and quality control.

Key words: flowers of Inula cappa, HS-SPME, GC-MS, volatile components, kovats retention indices

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