关键词: 雷山髭蟾（Leptobrachium leishanense）, 营养成分, 氨基酸, 营养评价

Abstract: To understand the nutritional status of Vibrissaphora leishanensis and provide the reference for its resource development， utilization and protection， general nutrients and amino acids of Vibrissaphora leishanensis were determined. The experimental results showed that：The contents of water， crude protein， crude fat， crude ash and dietary fiber of male and female Vibrissaphora leishanensis respectively were 80.84% and 84.12%、17.09% and 13.23%、1.25% and 1.01%、0.66% and 0.53%、0.25% and 0.19%， the energy was 2.64 MJ/kg and 2.06 MJ/kg. The composition of amino acids of in muscle male and female Vibrissaphora leishanensis were the same， 17 amino acids were detected. The total amino acids of male and female Vibrissaphora leishanensis respectively accounted for 87.659% and 87.566% of the dry samples. Seven essential amino acids （EAA） of male and female Vibrissaphora leishanensis respectively occuped 36.010% and 35.867% of total amino acids， the ratio of essential amino acids to nonessential amino acids was 84.70% and 84.46%， which were close to （or better than） the ideal mode of FAO /WHO （40% and 60%）. Delicious amino acid of male and female Vibrissaphora leishanensis respectively accounted for 27.345% and 27.367% of the total amino acids. Amino acid score （AAS） and chemical score （CS） indicated that the first limiting amino acid of Vibrissaphora leishanensis was valine， the second limiting amino acid was lysine. The essential amino acid index （EAAI） was respectively 88.63 and 88.13. There was no significant difference in nutritional composition of male and female Vibrissaphora leishanensis.

Key words: Vibrissaphora leishanensis, nutritent composition, amino acid, nutritive value