利用无菌苗叶片建立黑果枸杞高效繁殖体系

doi:10.14088/j.cnki.issn0439-8114.2021.09.028

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (9): 135-140.doi: 10.14088/j.cnki.issn0439-8114.2021.09.028

利用无菌苗叶片建立黑果枸杞高效繁殖体系

何凤明¹, 郭加元¹, 申婷¹, 马关雪¹, 严胜柒¹, 张云峰^1,2

1.云南师范大学生命科学学院,昆明 650500;
2.生物能源持续开发利用教育部工程研究中心,昆明 650500

收稿日期:2020-07-13 发布日期:2021-05-14
通讯作者: 张云峰,男,教授,主要从事植物资源与植物遗传方面的研究,（电子信箱）zhyunfen001@163.com。
作者简介:何凤明（1992-）,女,重庆巫山人,硕士,研究方向为生物技术遗传,（电话）18487182005（电子信箱）981301184@qq.com。
基金资助:
国家自然科学基金项目（30660075; 31960066）; 云南省科技厅社会发展科技计划基础研究面上项目（2009CD052）; 云南省基础研究计划资助项目（2011FA016）

Establishment of high-efficiency propagation system using sterile seedling leaves in Lycium ruthenicum Murr.

HE Feng-ming¹, GUO Jia-yuan¹, SHEN Ting¹, MA Guan-xue¹, YAN Sheng-qi¹, ZHANG Yun-feng^1,2

1. School of Life Science,Yunnan Normal University,Kunming 650500,China;
2. Bio Energy Sustainable Development and Utilization of Engineering Research Center of the Ministry of Education,Kunming 650500,China

Received:2020-07-13 Published:2021-05-14

摘要/Abstract

摘要： 试验在对单粒黑果枸杞（Lycium ruthenicum Murr.）花青素测定的基础上,以黑果枸杞无菌苗为外植体,利用响应面设计Design-Expert软件就激素浓度及C源组合对诱导响应率、愈伤诱导率及植株形成率的影响进行了研究。结果表明,诱导及愈伤诱导的最佳条件为6-BA 0.92 mg/L、NAA 0.45 mg/L、C源组合蔗糖为2.26%;而植株形成率的最佳条件为6-BA 1.15 mg/L、NAA 0.38 mg/L、C源组合蔗糖 2.93%。愈伤诱导过程中碳源组合与激素间存在较强的互作,组织切片所采取的诱导途径可能是胚状体再生途径,RAPD 检验显示后代的变异率低,表明利用该繁殖体所培育的幼苗后代可维持母系的优良性状。

关键词: 黑果枸杞（Lycium ruthenicum Murr.）, 胚状体再生途径, 一次成苗, 响应面设计, 生根

Abstract: In present experiment, based on the measurement of anthocyanins content in single dried berry, the aseptic leaves derived from the sterile seedlings of L.ruthenicum Murr. which germinated on MS medium were used as explants, and optimization condition of hormone concentration and C source combination was selected for response frequency, callus inducing frequency and plantlet forming frequency using the response surface design Design-Expert software. The results showed that the best condition for response frequency and callus induction frequency was 6-BA 0.92 mg/L, NAA 0.45 mg/L, and C source for sucrose 2.26%, respectively; And best condition for plantlet formation frequency was 6-BA 1.15 mg/L, NAA 0.38 mg/L, and C source sucrose 2.93%, respectively. Result also showed that there was a strong interaction between the carbon source combination and hormones during the callus induction process. Tissue sections showed that the the way for plantlet forming induction or shoot regeneration adopted in the experiment may be the somatic embryogenesis, and RAPD test of the offspring regeneration obtained in present showed extremely low rate of variation which suggested the excellent characteristic of mother plant would maintain in descendant derived from this propagation system.

Key words: Lycium ruthenicum Murr., somatic embryogenesis regeneration, one step for plantlet formation, response surface analysis, rooting

中图分类号:

S888.2

何凤明, 郭加元, 申婷, 马关雪, 严胜柒, 张云峰. 利用无菌苗叶片建立黑果枸杞高效繁殖体系[J]. 湖北农业科学, 2021, 60(9): 135-140.

HE Feng-ming, GUO Jia-yuan, SHEN Ting, MA Guan-xue, YAN Sheng-qi, ZHANG Yun-feng. Establishment of high-efficiency propagation system using sterile seedling leaves in Lycium ruthenicum Murr.[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 135-140.

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利用无菌苗叶片建立黑果枸杞高效繁殖体系

Establishment of high-efficiency propagation system using sterile seedling leaves in Lycium ruthenicum Murr.

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