湖北农业科学 ›› 2021, Vol. 60 ›› Issue (9): 21-25.doi: 10.14088/j.cnki.issn0439-8114.2021.09.004

• 育种·栽培 • 上一篇    下一篇

申嗪霉素-缬氨酸耦合物在小麦植株上的传导性研究

董倩a, 赵炽娜a, 李俊凯a,b   

  1. 长江大学,a.农学院; b.农药研究所,湖北 荆州 434025
  • 收稿日期:2021-03-01 发布日期:2021-05-14
  • 通讯作者: 李俊凯,教授,主要从事新农药研究与开发,(电子信箱)junkaili@sina.com。
  • 作者简介:董 倩(1996-),女,湖北黄冈人,在读硕士研究生,研究方向为新农药研究与开发,(电话)13697318201(电子信箱)245923698@qq.com;共同第一作者,赵炽娜(1993-),女,广西贵港人,硕士,主要从事新农药研究与开发,(电话)18271008030(电子信箱)1183824485@qq.com。
  • 基金资助:
    国家自然科学基金项目(31672069)

Study on the systemicity of phenazine-1-carboxylic acid-valine conjugates in wheat plants

DONG Qiana, ZHAO Chi-naa, LI Jun-kaia,b   

  1. a. College of Agriculture;
    b. Institute of Pesticides, Yangtze University, Jingzhou 434025, Hubei, China
  • Received:2021-03-01 Published:2021-05-14

摘要: 为了明确申嗪霉素-缬氨酸耦合物在小麦(Triticum aestivum L.)植株中的内吸传导特性,以申嗪霉素-缬氨酸耦合物为供试药剂,利用HPLC-MS/MS方法定量分析了在分蘖期小麦叶部施药处理方式下药剂进入植株后的分布和累积情况。结果表明,对分蘖期的小麦植株,使用药剂浓度200 μmol/L处理叶片时,在小麦的根部均能检测到耦合物的含量,且申嗪霉素-L-缬氨酸耦合物(PCA-L-Val)于处理18 h后在小麦根部的含量最高,为15.50 μmol/kg,而申嗪霉素-D-缬氨酸耦合物(PCA-D-Val)在同一时刻在小麦根部的含量也达到最大值,为11.37 μmol/kg。同时在小麦的分蘖叶片中也检测到耦合物的含量,且PCA-L-Val叶面处理小麦3 h后该化合物在分蘖叶片中的含量达到最大值,为8.74 μmol/kg,而PCA-D-Val在同一时刻也达到最大值,为4.54 μmol/kg。由此可知,申嗪霉素-缬氨酸耦合物具有在小麦韧皮部传导的特性,还具备了由主茎向分蘖间传导的特性,且PCA-L-Val的传导性强于PCA-D-Val。

关键词: 申嗪霉素-缬氨酸耦合物, 小麦(Triticum aestivum L.)植株, 内吸传导性

Abstract: To clarify the uptake and translocation ability of phenazine-l-carboxylic acid-valine(PCA-Val) conjugate in wheat plants, using PCA-Val as a supplied test fungicides, the transportation and the contents of PCA-Val in leaves and roots of wheat plants were determined by ultra performance liquid chromatography-tandem mass spectrometry method after it was applied to leaves. The results showed that, for wheat plants at the tillering stage, when the concentration of the tested agents was 200 μmol/L, the content of the compounds could be detected in the roots of wheat plants, and the content of PCA-L-Val in the roots reached its peak of 15.50 μmol/kg after treatment for 18 h, and that of PCA-D-Val reached the peak at the same time, which was 11.37 μmol/kg. In addition, the conjugates could also be detected in the tiller leaves of wheat, and the content of PCA-L-Val in the tiller reached its peak of 8.74 μmol/kg after treatment for 3 h, while PCA-D-Val reached its peak of 4.54 μmol/kg at the same time. Study proves that, the PCA-valine conjugates has phloem mobility in wheat plants,and also has the particular systemicity from the main stem to the tillers, the systemic mobility of valine conjugates in L configuration were significantly higher than those in D configuration.

Key words: phenazine-l-carboxylic acid -valine conjugate, wheat(Triticum aestivum L.) plants, uptake and translocation

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