探讨DNA条形码对摇蚊属物种界定的有效性

doi:10.14088/j.cnki.issn0439-8114.2021.05.028

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (5): 144-148.doi: 10.14088/j.cnki.issn0439-8114.2021.05.028

探讨DNA条形码对摇蚊属物种界定的有效性

余海军, 王茜

天津农学院水产学院/天津市水生生态及养殖重点实验室,天津 300384

收稿日期:2020-06-29 出版日期:2021-03-10 发布日期:2021-03-22
通讯作者: 王茜（1971-）,女,副教授,博士,主要从事水生动物及昆虫分子系统学的研究,（电话）13682014584（电子信箱）wqgt1999@163.com。
作者简介:余海军（1994-）,男,贵州雷山人,在读硕士研究生,研究方向为分子系统学,（电话）18322040463（电子信箱）yhj18322040463@163.com;
基金资助:
国家自然科学基金项目（NSFC31672264）

Exploring the utility of DNA barcoding in species identification of Chironomus

YU Hai-jun, WANG Qian

College of Fishery, Tianjin Agricultural University Key Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, Tianjin 300384, China

Received:2020-06-29 Online:2021-03-10 Published:2021-03-22

摘要/Abstract

摘要： 以摇蚊属的 12种物种为研究对象,结合Barcode of Life Database（BOID）和Genbank数据库下载的条形码数据,采用Automatic Barcode Gap Discovery（ABGD）和邻接法（Neighbor joining, NJ）对所获取的 749条DNA条形码进行分析,探究DNA条形码在摇蚊属物种界定的有效性。结果表明,基于ABGD的分析,显示物种遗传距离之间存在空白区;通过分析邻接树,得到60个分子分类操作单元,与形态学鉴定结果一致,并将数据库中Chironomus sp.TE11修正为Chironomus pseudothummi。研究在一定程度上补充了中国摇蚊科DNA条形码研究,也为摇蚊科DNA条形码数据库的构建和完善提供了数据支持。

关键词: 摇蚊属, DNA条形码, 物种界定

Abstract: The research analyzed 749 DNA barcodes collected the 12 species of Chironomus species and downloaded barcodes in BOLD and Genbank database by ABGD （Automatic Barcode Gap Discovery） and NJ （Neighbor-joining）. The result showed that ABGD analysed genetic distance, existed “DNA barcode gap”. The NJ tree supported 60 molecular operational taxonomic units, and keep conformed to morphological identification.In addition, based on the data are found, Chironomus sp.TE11 from public library should be Chironomus pseudothummi. Our result is an important supplement for the Chinese DNA barcodes study of Chironominae, and provide data support for establishing and perfecting the Chinese DNA barcodes database of Chironominae.

Key words: Chironomus, DNA barcoding, species identification

中图分类号:

Q969.44
Q523

余海军, 王茜. 探讨DNA条形码对摇蚊属物种界定的有效性[J]. 湖北农业科学, 2021, 60(5): 144-148.

YU Hai-jun, WANG Qian. Exploring the utility of DNA barcoding in species identification of Chironomus[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(5): 144-148.

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探讨DNA条形码对摇蚊属物种界定的有效性

Exploring the utility of DNA barcoding in species identification of Chironomus

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