低温胁迫下两叶一心期棉花叶片的生理响应和转录组分析

doi:10.14088/j.cnki.issn0439-8114.2020.24.038

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (24): 169-176.doi: 10.14088/j.cnki.issn0439-8114.2020.24.038

低温胁迫下两叶一心期棉花叶片的生理响应和转录组分析

王晓曼^1,2,3, 王琼珊^1,2,3, 王孝刚^1,2,3, 张教海^1,2,3, 张友昌^1,2,3, 张兴中^1,2,3, 别墅^1,2,3, 夏松波^1,2,3

1.湖北省农业科学院经济作物研究所,武汉 430064;
2.农业农村部长江中游棉花生物学与遗传育种重点实验室,武汉 430064;
3.湖北省农业科技创新中心,武汉 430064

收稿日期:2020-10-26 出版日期:2020-12-25 发布日期:2021-01-21
通讯作者: 夏松波,副研究员,主要从事棉花栽培研究工作,(电话)13667267696;别墅,研究员,主要从事棉花栽培研究工作,(电子信箱)bieshu02@163.com。
作者简介:王晓曼(1990-),女,山西忻州人,硕士,主要从事生物技术研究工作,(电话)15827491198,(电子信箱)1216080733@qq.com
基金资助:
棉花轻简高效栽培技术集成与示范项目(2020YFD1001000); 现代农业产业技术体系专项; 湖北省农业科技创新中心项目(2019-620-000-06)

Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature

WANG Xiao-man^1,2,3, WANG Qiong-shan^1,2,3, WANG Xiao-gang^1,2,3, ZHANG Jiao-hai^1,2,3, ZHANG You-chang^1,2,3, ZHANG Xing-zhong^1,2,3, BIE Shu^1,2,3, XIA Song-bo^1,2,3

1. Institute of Cash Crops, Hubei Academy of Agricultural Sciences,Wuhan 430064, China;
2. Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjiang River, Ministry of Agriculture and Rural Affairs,Wuhan 430064, China;
3. Hubei Agricultural Science and Technology Innovation Center,Wuhan 430064, China

Received:2020-10-26 Online:2020-12-25 Published:2021-01-21

摘要/Abstract

摘要： 以CN01和SJB016(耐低温)为材料,研究了低温处理对两叶一心期棉花叶片的影响。测定了25 ℃(CK)和12 ℃(低温)处理0、3、6、12 、24、48和72 h后CN01和SJB016中丙二醛(MDA)、可溶性糖(SS)和脯氨酸(Pro)含量的变化。研究发现,与25 ℃相比,12 ℃处理后CN01和SJB016中MDA、SS和Pro含量均上升。与CN01相比,低温处理后SJB016中MDA含量上升幅度较小,SS含量上升幅度较大,而Pro含量上升幅度较小。结合上述3个指标,发现12 ℃处理6 h棉花叶片的生理指标变化最明显。对12 ℃处理6 h的两叶一心期棉花叶片进行了RNA-seq。结果表明,与25 ℃相比,12 ℃处理后与光合作用相关的基因在CN01中均表现为上调,而光系统Ⅱ放氧复合物(PsdP、PsdQ)等基因在SJB016中表现为下调。与植物激素相关差异的基因在CN01和SJB016中表现为上调基因多于下调基因,其中乙烯响应转录因子ERF053、ERF020 、ERF061表现为显著上调。这些基因可能对提高棉花的抗寒性有重要作用。

关键词: 棉花叶片, 生理响应, 转录组, 基因表达, 低温胁迫

Abstract: CN01 and SJB016 (a low temperature resistant cotton) were used as materials to study the effects of low temperature treatment on cotton in the two-leaf period. Firstly, We separately measured the contents of malondialdehyde (MDA), soluble sugar (SS) and proline (Pro) in CN01 and SJB016 at 25 ℃ (CK) and 12 ℃ (low temperature treatment) for 0, 3, 6, 12, 24, 48 and 72 h. It was found that the contents of MDA, SS, and Pro in CN01 and SJB016 increased after 12 ℃ treatment compared with 25 ℃. After low temperature treatment, the content of MDA in SJB016 increased less than CN01, the content of SS in SJB016 increased larger than CN01, and the content of Pro in SJB016 increased less than CN01. Transcriptome analysis of cotton leaves in two-leaf period after low temperature treatment for 6 hours showed that the photosynthesis-related genes were up-regulated in CN01 after 12 ℃ treatment, while the genes of photosystem II oxygen-releasing complexes (PsdP, PsdQ, et al) were down-regulated in SJB016, compared with 25 ℃. The up-regulated differentially expressed genes associated with phytohormone were more than down-regulated genes in CN01 and SJB016, and ethylene-responsive transcription factors ERF053, ERF020, and ERF061 significantly up-regulated. These genes are deemed to play an important role in improving the cold resistance of cotton.

Key words: cotton leaf, physiological response, transcriptome, gene expression, low temperature treatment

中图分类号:

Q945.78

王晓曼, 王琼珊, 王孝刚, 张教海, 张友昌, 张兴中, 别墅, 夏松波. 低温胁迫下两叶一心期棉花叶片的生理响应和转录组分析[J]. 湖北农业科学, 2020, 59(24): 169-176.

WANG Xiao-man, WANG Qiong-shan, WANG Xiao-gang, ZHANG Jiao-hai, ZHANG You-chang, ZHANG Xing-zhong, BIE Shu, XIA Song-bo. Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(24): 169-176.

参考文献

[1] YOU W U, ZHAO W Q, MENG Y L, et al.Relationships between temperature-light meteorological factors and seedcotton biomass per boll at different boll positions[J]. Journal of integrative agriculture, 2018,17(6):1315-1326.
[2] 高艳,唐建卫,殷贵鸿,等.倒春寒发生时期和次数对冬小麦产量性状的影响[J]. 麦类作物学报,2015,35(5): 687-692.
[3] 王钰静,谢磊,李志博,等.低温胁迫对北疆棉花种子萌发的影响及其耐冷性差异评价[J]. 种子,2014,33(5): 74-77.
[4] 蔡永萍. 植物生理学实验指导[M].北京:中国农业大学出版社,2014.
[5] 郑国华,张贺英,钟秀容.低温胁迫下枇杷叶片细胞超微结构及膜透性和保护酶活性的变化[J]. 中国生态农业学报,2009,17(4): 739-745.
[6] STEVENS R G,CREISSEN G P,MULLINEAUX P M.Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress[J]. Plant molecular biology,1997,35(5): 641-654.
[7] 朱政,蒋家月,江昌俊,等.低温胁迫对茶树叶片SOD、可溶性蛋白和可溶性糖含量的影响[J]. 安徽农业大学学报,2011, 38(1): 24-26.
[8] 宁露云,包满珠,张蔚. 低温胁迫对矮牵牛H株系花青素、游离脯氨酸及可溶性糖含量的影响[J]. 湖北农业科学,2016,55(6): 1500-1503.
[9] 王孝宣,李树德,东惠茹,等.番茄品种耐寒性与ABA和可溶性糖含量的关系[J]. 园艺学报,1998,25(1): 57-61.
[10] 王荣富. 植物抗寒指标的种类及其应用[J]. 植物生理学通讯,1987,23(3): 49-55.
[11] YELENOSKY G.Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes[J]. Plant Physiol,1979,64(3):425-427.
[12] 李育军,赵玉田,常汝镇,等. 大豆萌发期对6 ℃低温的反应[J]. 大豆科学,1990(2):136-139.
[13] 武辉. 低温胁迫下棉花幼苗生理生化响应机制的研究[D].乌鲁木齐:新疆农业大学,2012.
[14] ALLEN D J,ORT D R.Impacts of chilling temperatures on photosynthesis in warm-climate plants[J]. Trends in plant science,2001, 6(1): 36-42.
[15] 秦玉芝,陈珏,邢铮,等.低温逆境对马铃薯叶片光合作用的影响[J]. 湖南农业大学学报(自然科学版),2013,39(1): 26-30.
[16] GREER D H,LAING W A.Photoinhibition of photosynthesis in intact kiwifruit(Actinidia deliciosa) leaves: Recovery and its dependence on temperature[J]. Planta,1988,174(2):159-165.
[17] ROACH T,KRIEGER-LISZKAY A.The role of the PsbS protein in the protection of photosystems I and II against high light in Arabidopsis thaliana[J]. Biochimica Et Biophysica Acta,2012, 1817(12): 2158-2165.
[18] 安飞飞,李庚虎,陈霆,等. 低温胁迫对木薯叶片叶绿素荧光参数及PSⅡ相关蛋白表达水平的影响[J]. 湖南农业大学学报(自然科学版),2014,40(2): 148-152.
[19] CHEN Y E,ZHANG C M,SU Y Q,et al.Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat[J]. Environmental & experimental botany,2017,135(3):45-55.
[20] 岳丹,王有科.杏树内源激素含量与抗寒性关系研究[J]. 安徽农业科学,2008,36(23): 9951-9952.
[21] RIKIN A,WALDMAN M,RICHMOND A E,et al.Hormonal regulation of morphogenesis and cold-resistance[J]. Journal of experimental botany, 1975,26(2):175-183.
[22] YUAN H,ZHAO K,LEI H,et al.Genome-wide analysis of the GH3 family in apple (Malus×Domestica)[J]. Bmc Genomics,2013,14(1): 297.
[23] DU H,LIU H,XIONG L.Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice[J]. Frontiers in plant science,2013,4(397): 397.
[24] SHI Y, TIAN S, HOU L, et al.Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis.[J]. Plant cell,2012,24(6):2578-2595.
[25] CHEN N,YANG Q,SU M,et al.Cloning of six ERF family transcription factor genes from peanut and analysis of their expression during abiotic stress[J].Plant molecular biology reporter,2012, 31(6): 1415-1425.
[26] TANG W,NEWTON R J,LI C,et al.Enhanced stress tolerance in transgenic pine expressing the pepper CaPF1 gene is associated with the polyamine biosynthesis[J]. Plant cell reports,2007, 26(1): 115-124.
[27] 刘晓月,张婷,黄立钰,等. 水稻MYB转录因子冷胁迫表达谱分析[J]. 分子植物育种,2014,12(1): 9-16.
[28] JIANG Y,DUAN Y,YIN J,et al.Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses[J]. Journal of experimental botany,2014, 65(22): 6629-6644.
[29] ZOU C,JIANG W,YU D.Male gametophyte-specific WRKY34 transcription factor mediates cold sensitivity of mature pollen in Arabidopsis[J]. Journal of experimental botany,2010,61(14): 3901-3914.

低温胁迫下两叶一心期棉花叶片的生理响应和转录组分析

Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature

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