鸡HDLBP基因SNP鉴定及其与蛋用性能关联分析

doi:10.14088/j.cnki.issn0439-8114.2020.24.033

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (24): 144-147.doi: 10.14088/j.cnki.issn0439-8114.2020.24.033

鸡HDLBP基因SNP鉴定及其与蛋用性能关联分析

黄涛¹, 程璐², 莫长欢², 王孝忠³, 申杰¹, 皮劲松¹, 龚炎长²

1.湖北省农业科学院畜牧兽医研究所/湖北省农业科技创新中心/动物胚胎与分子育种湖北省重点实验室,武汉 430064;
2.华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉 430070;
3.宜昌市畜牧兽医中心,湖北宜昌 443000

收稿日期:2020-09-28 出版日期:2020-12-25 发布日期:2021-01-21
通讯作者: 龚炎长,教授,主要从事家禽遗传育种研究,(电子信箱)poultry@mail.hau.edu.cn。
作者简介:黄涛(1989-),男,河南信阳人,博士,主要从事家禽遗传育种研究,(电话)13100655209(电子信箱)huangtao214@126.com
基金资助:
国家自然科学基金项目(31772584); 国家肉鸡产业技术体系建设专项资金项目(CARS-41-Z14)

Identification of HDLBP SNPs and its association with egg performance traits

HUANG Tao¹, CHENG Lu², MO Chang-huan², WANG Xiao-zhong³, SHEN Jie¹, PI Jin-song¹, GONG Yan-zhang²

1. Institute of Animal Husbandry and Vetervinary,Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding, Wuhan 430064, China;
2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education,Huazhong Agricultural University, Wuhan 430070, China;
3. Animal Husbandry and Veterinary Center of Yichang City,Yichang 443000,Hubei, China

Received:2020-09-28 Online:2020-12-25 Published:2021-01-21

摘要/Abstract

摘要： 从HDLBP转录本(NM_205096.2)中鉴定6个同义突变的SNPs,在欣华鸡的关联分析发现C3381T和T387C与开产日龄(AFE)和蛋重(EW)显著关联(P<0.05),表明该基因可能是蛋用性状相关的候选基因。从小白卵泡(SWF)到小黄卵泡(SYF)中,HDLBP表达水平在膜层和颗粒层中逐渐升高。在排卵前期,F3到F2的颗粒层中HDLBP mRNA水平显著下降(P<0.05)。此外,EGF、VEGF、FSH在不同颗粒细胞生长阶段对HDLBP调控具有剂量依赖性,Follistatin对HDLBP mRNA表达没有明显影响。

关键词: 高密度脂蛋白结合蛋白, SNP, 卵泡

Abstract: 6 SNPs in the full length of HDLBP transcript were identified as a synonymous mutation in adult hens. Association between two SNPs and egg performance traits were analyzed in Xinhua E-strain chickens, which showed that they were highly significantly associated with AFE and EW (P<0.05), suggesting that it was a candidate gene related with egg production. The HDLBP mRNA in different sized follicles showed that the expression was progressively increased in follicles from undifferentiated follicles to SYFs, and maintain stable in pre-ovulatory follicle. Besides, FSH, EGF, VEGF with dosage dependent could regulate the expression of HDLBP in granulosa cells of phases, while Follistatin showed no effect.

Key words: HDLBP, SNP, follicle

中图分类号:

S831.2

黄涛, 程璐, 莫长欢, 王孝忠, 申杰, 皮劲松, 龚炎长. 鸡HDLBP基因SNP鉴定及其与蛋用性能关联分析[J]. 湖北农业科学, 2020, 59(24): 144-147.

HUANG Tao, CHENG Lu, MO Chang-huan, WANG Xiao-zhong, SHEN Jie, PI Jin-song, GONG Yan-zhang. Identification of HDLBP SNPs and its association with egg performance traits[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(24): 144-147.

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鸡HDLBP基因SNP鉴定及其与蛋用性能关联分析

Identification of HDLBP SNPs and its association with egg performance traits

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