EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响

doi:10.14088/j.cnki.issn0439-8114.2020.23.029

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (23): 130-133.doi: 10.14088/j.cnki.issn0439-8114.2020.23.029

EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响

李振军, 吴涵, 杨烨

长江大学动物科学学院,湖北荆州 434025

收稿日期:2020-10-16 出版日期:2020-12-10 发布日期:2020-12-30
作者简介:李振军(1994-),男,河北迁西人,在读硕士研究生,研究方向为动物营养与饲料专业,(电话)15927737031(电子信箱)1029918072@qq.com
基金资助:
国家自然科学基金项目(31472117)

Effect of epigallocatechin gallate on lipid deposition and liver anti-oxidative capacity in heat-stressed broilers

LI Zhen-jun, WU Han, YANG Ye

College of Animal Science,Yangtze University,Jingzhou 434025,Hubei,China

Received:2020-10-16 Online:2020-12-10 Published:2020-12-30

摘要/Abstract

摘要： 为阐明EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响,将192只2周龄肉鸡分为4个组别:对照组为适宜温度组(28 ℃,TN组),饲喂基础日粮,试验组为循环高温组(7:00到19:00 35 ℃,19:00到7:00 28 ℃,HS组),试验组日粮在基础日粮中分别添加0 mg/kg EGCG(HS0组)、300 mg/kg EGCG(HS300组)、600 mg/kg EGCG(HS600组),试验期3周。结果表明,与对照组相比,热应激(HS0组)肉鸡的末重、日增重和日采食量显著降低,肝脏的SOD、GSH-Px活性、Nrf2 mRNA表达水平显著降低(P<0.05),而肝脏中MDA含量、LITAF、NF-κB、FAS、SREBP1 mRNA表达水平以及脂肪沉积显著增加(P<0.05);而热应激肉鸡在饲料中添加EGCG后,上述指标得到明显改善,与热应激组(HS0组)相比,添加EGCG(HS300和HS600)显著增加了肝脏SOD、GSH-Px、CAT活性以及Nrf2 mRNA表达水平(P<0.05),同时肝脏MAD含量、脂肪沉积以及LITAF、NF-κB、FAS、SREBP1 mRNA表达水平,同时改善生产性能。EGCG通过提高肝脏的抗氧化性能,缓解热应激对脂肪沉积及生产性能的影响。

关键词: EGCG, 抗氧化性能, 脂肪沉积, 热应激, 肉鸡

Abstract: This study aims to investigate the effects of EGCG on the lipid deposition and liver anti-oxidative capacity of broilers under heat stress.One hundred and ninety-two 2-wk-old broilers were divided into four groups with 6 replicates per group and 8 chickens per replicate.One thermoneutral control group (28°C,TN group),which was fed the basal diet;And three cyclic high-temperature groups (35°C from 7:00 to 19:00;28°C from 19:00 to 7:00,heat stress (HS) group),which were fed the basal diet with added EGCG at doses of 0 mg/kg (HS0 group),300 mg/kg (HS300 group),and 600 mg/kg (HS600 group),respectively.The growth performance and liver metabolism and lipid deposition indices were performed at 35 d of age.The results showed that heat stress significantly reduced body weight,daily weight gain,daily feed intake,and decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),and the Nrf2 mRNA expression in liver,and increase significantly the levels of malondialdehyde (MDA) and the expression of LITAF,NF-κB,FAS,SREBP1 mRNA and the lipid deposition compared with TN group.EGCG (HS300 and HS600 group) increased the activities of SOD and GSH-Px and catalase (CAT),and increased the Nrf2 mRNA expression,and decreased the MAD contents,and reduced the lipid deposition and expression of LITAF,NF-κB,FAS,SREBP1 mRNA.The results of this study show that EGCG can improve liver antioxidative capacity to alleviate oxidative damage caused by heat stress.

Key words: EGCG, antioxidative capacity, lipid deposition, heat stress, broilers

中图分类号:

S831

李振军, 吴涵, 杨烨. EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响[J]. 湖北农业科学, 2020, 59(23): 130-133.

LI Zhen-jun, WU Han, YANG Ye. Effect of epigallocatechin gallate on lipid deposition and liver anti-oxidative capacity in heat-stressed broilers[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 130-133.

参考文献

[1] BERRONG S L,WASHBURN K W.Effects of genetic variation on total plasma protein,body weight gains,and body temperature responses to heat stress[J].Poultry science,1998,77(3):379-385.
[2] DEMBINSKA-KIEC A,MYKKÄNEN O,KIEC-WILK B,et al.Antioxidant phytochemicals against type 2 diabetes[J].British journal of nutrition,2008,99:109-117.
[3] HAN X,LOA T.Dietary polyphenols and their biological significance[J].International journal of molecular sciences,2007,8:950-988.
[4] GU X H,HAO Y,WANG X L.Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers:Intestinal oxidative stress[J].Poultry science,2012,91(4):790-799.
[5] KALUPAHANA N S,MOUSTAID-MOUSSA N.The adipose tissue renin-angiotensin system and metabolic disorders:A review of molecular mechanisms[J].Critical reviews in biochemistry and molecular biology,2012,47(4):379-390.
[6] LI Y,HUANG Y,PIAO Y,et al.Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis[J].Reproductive biology and endocrinology,2013,11:23.
[7] KENNETH J L,THOMAS D S.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J].Methods,2001,25:402-408.
[8] LIU L L,HE J H,XIE H B,et al.Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens[J].Poultry science,2014,93(1):54-62.
[9] LU Z,HE X,MA B,et al.Chronic heat stress impairs the quality of breast-muscle meat in broilers by affecting redox status and energy-substance metabolism[J].Journal of agricultural and food chemistry,2017,65(51):11251-11258.
[10] MURPHY M P.How mitochondria produce reactive oxygen species[J].Biochemical journal,2009,417:1-13.
[11] ORHAN C,TUZCU M,GENCOGLU H,et al.Epigallocatechin-3-gallate exerts protective effects against heat stress through modulating stress-responsive transcription factors in poultry[J].British poultry science,2013,54(4):447-453.
[12] QU H,DONKIN S S,AJUWON K M.Heat stress enhances adipogenic differentiation of subcutaneous fat depot-derived porcine stromovascular cells[J].Journal of animal science,2015,93(8):3832-3842.
[13] SAHIN K,ORHAN C,TUZCU M,et al.Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails[J].Poultry science,2010,89(10):2251-2258.
[14] TAN G Y,YANG L,FU Y Q,et al.Effects of different acute high ambient temperatures on function of hepatic mitochondrial respiration,antioxidative enzymes,and oxidative injury in broiler chickens[J].Poultry science,2010,89(1):115-122.
[15] YANG L,TAN G Y,FU Y Q,et al.Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration,ROS production and lipid peroxidation in broiler chickens[J].Comparative biochemistry and physiology,Part C,2010,151(2):204-208.
[16] ZAEFARIAN F,ABDOLLAHI M R,COWIESON A,et al.Avian liver:The forgotten organ[J].Animals,2019,9(2):63.
[17] ZHANG Z Y,JIA G Q,ZUO J J,et al.Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat[J].Poultry science,2012,91(11):2931-2937.

EGCG对热应激肉鸡脂肪沉积与肝脏抗氧化性能的影响

Effect of epigallocatechin gallate on lipid deposition and liver anti-oxidative capacity in heat-stressed broilers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

编辑推荐

Metrics

本文评价

[1]	王丹, 崔卫涛, 王纯, 况世昌, 许青荣, 肖运才, 李自力, 周祖涛. 发酵中药制剂对肉鸡生产性能、免疫功能、肠道形态及盲肠菌群影响的研究[J]. 湖北农业科学, 2021, 60(24): 164-168.
[2]	何菊, 李筱雯, 崔卫涛, 郭杰, 王纯, 肖运才, 李自力, 周祖涛. 丁酸梭菌CB1制剂对肉鸡生产性能、肠道形态和菌群的影响[J]. 湖北农业科学, 2021, 60(17): 93-97.
[3]	申杰, 黄涛, 吴艳, 皮劲松, 潘爱銮, 梁振华, 孙静, 张昊, 蒲跃进. 江汉鸡与隐性白羽肉鸡及其正反交后代体尺和屠宰性状比较研究[J]. 湖北农业科学, 2020, 59(23): 122-125.
[4]	李心语, 安清明, 王大会, 贺花, 冯紫婷, 文逸凡, 雷初朝, 陈宏, 黄永震. miRNA对畜禽脂肪沉积的影响及在遗传育种中的应用[J]. 湖北农业科学, 2020, 59(13): 14-18.
[5]	李家凯, 郭康康, 刘丽娜, 温国元, 罗青平, 李自力, 董世山, 邵华斌, 赵宗正. 肉鸡与蛋鸡H5、H7、H9亚型禽流感病毒抗体水平调查[J]. 湖北农业科学, 2020, 59(11): 104-106.
[6]	马志禹, 张莹, 陈风雷, 杨盛, 乔文娜, 胡佳慧, 张金龙, 雷治海. 热应激对兔下丘脑-垂体-睾丸轴NMB及其受体基因表达和睾酮分泌的影响[J]. 湖北农业科学, 2019, 58(12): 141-146.
[7]	潘爱銮, 申杰, 杨林, 蒲跃进, 吴艳, 皮劲松, 王德才, 孙静, 梁振华, 张昊, 杜金平. 商品肉鸡1234精准养殖工艺研究[J]. 湖北农业科学, 2018, 57(24): 116-119.
[8]	李俊, 程业飞, 苏越, 陶正国, 周迪, 周岩民, 温超. 阿朴酯和斑蝥黄复合对蛋鸡抗氧化功能、蛋品质和蛋黄色素沉积的影响[J]. 湖北农业科学, 2018, 57(18): 88-91.
[9]	王芹, 赵云青, 李述举, 王春光, 龚成云, 李传恺, 朱雯. 蒸气杀青时间对白茶花品质的影响[J]. 湖北农业科学, 2018, 57(12): 73-76.