枯草期放牧东宝黑头羊瘤胃内环境参数及瘤胃细菌多样性分析

doi:10.14088/j.cnki.issn0439-8114.2020.23.026

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (23): 116-121.doi: 10.14088/j.cnki.issn0439-8114.2020.23.026

枯草期放牧东宝黑头羊瘤胃内环境参数及瘤胃细菌多样性分析

张年^1,2, 杨前平¹, 陶虎¹, 熊琪¹, 李晓锋¹, 陈明新¹, 索效军¹

1.湖北省农业科学院畜牧兽医研究所,武汉 430064;
2.动物胚胎工程及分子育种湖北省重点实验室,武汉 430064

收稿日期:2020-07-22 出版日期:2020-12-10 发布日期:2020-12-30
通讯作者: 索效军(1977-),男,山西忻州人,副研究员,硕士,主要从事草食家畜育种、开发利用与推广等,(电话)13297963464(电子信箱)suoxiaojun@126.com
作者简介:张年(1979-),女,河南沈丘人,副研究员,硕士,主要从事草食家畜繁育、疫病防治等,(电话)13554336864(电子信箱)zn991830@163.com
基金资助:
湖北省农业科技创新中心项目(2020-620-004-002);湖北省农业科技创新行动项目“畜禽优质增效与绿色养殖技术集成创新与示范”(2018SKJCX05)

Analysis on ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period

ZHANG Nian^1,2, YANG Qian-ping¹, TAO Hu¹, XIONG Qi¹, LI Xiao-feng¹, CHEN Ming-xin¹, SUO Xiao-jun¹

1. Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;
2. Key Laboratory of Animal Embryonic Engineering and Molecular Breeding in Hubei Province,Wuhan 430064,China

Received:2020-07-22 Online:2020-12-10 Published:2020-12-30

摘要/Abstract

摘要： 试验旨在研究枯草期放牧东宝黑头羊瘤胃内环境参数及瘤胃细菌多样性。采集枯草期牧草样品,测定常规营养成分含量,选取6只体重为(45.24±1.25) kg的2岁全年放牧的东宝黑头羊(公母各半),进行耳牌标记,在枯草期放牧前空腹经口腔采集瘤胃液,用于分析瘤胃内环境参数和瘤胃细菌多样性。结果表明,东宝黑头羊瘤胃液pH为6.65,氨态氮(NH₃-N)浓度50.30 mg/L,乙酸/丙酸4.43;6个样本共获得290 868条高质量16S rDNA序列,479.67个分类操作单元(OTU),东宝黑头羊瘤胃细菌丰富度指数Ace指数、Chao1指数分别为523.31、533.79,α-多样性指数Shannon指数、Simpson指数分别为6.39、0.96;经物种注释,共有15个门23个纲29个目34个科87个属被鉴定。在门水平,拟杆菌门(Bacteroidetes,75.75%)、厚壁菌门(Firmicutes,15.62%)含量最多;在科水平,主要包括理研菌科(Rikenellaceae,45.89%)、普雷沃氏菌科(Prevotellaceae,19.18%);在属水平,理研菌科RC9肠道群(Rikenellaceae_RC9_ gut_group,45.64%)、普雷沃氏菌属(Prevotella_1,15.46%)是放牧东宝黑头羊瘤胃中的优势种群。结果证明,枯草期放牧东宝黑头羊瘤胃细菌多样性较低;乙酸/丙酸比值较高;东宝黑头羊瘤胃中最优势菌门为拟杆菌门,其次是厚壁菌门;理研菌科RC9肠道群是东宝黑头羊瘤胃中最优势细菌属。

关键词: 枯草期, 放牧东宝黑头羊, 瘤胃内环境, 细菌多样性, MiSeq

Abstract: The aim of this experiment was to study ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period.The forge grass were collected and the common nutrition contents were determined.Six healthy annual grazing Dongbao blackhead goats (3 males and 3 females) with the body weight of (45.24±1.25) kg were selected and marked using ear tags with numbers,and the rumen fluids of them were collected via oral cavity before free-range with empty stomach in grass withering period to measured the ruminal environment parameters and rumen microbial diversity.The results showed that the pH of rumen fluid of Dongbao blackhead goats was 6.65,the concentration of ammonia nitrogen (NH₃-N) was 50.30 mg/L,and the ratio of acetic acid/propionic acid was 4.43.A total of 290 868 valid 16S rDNA sequences and 479.67 operational taxonomic unit (OTU) across 6 samples were obtained.The richness indexes Ace and Chao1 indexes of ruminal bacterial were 533.79 and 523.31,respectively,and alpha diversity indexes Shannon and Simpson indexes were 6.39 and 0.96,respectively.The rumen bacteria of grazing Dongbao blackhead goats belonging to 15 phyla,23 classes,29 orders,34 families and 87 genera were identified via taxonomic summary.Among these rumen bacterial populations,the dominant bacterial phyla were Bacteroidetes (75.75%) and Firmicutes (15.62%).The dominant bacterial families were Rikenellaceae (45.89%),Prevotellaceae (19.18%).Rikenellaceae_RC9_gut_group (45.64%) and Prevotella_1 (15.46%) were the dominant bacterial genera.In conclusion,the rumen bacterial diversity of grazing Dongbao blackhead goats was low in grass withering period,and the ratio of acetic acid/propionic acid was high.Bacteroidetes is the predominant phylum and Firmicutes is the second most abundant phylum.Rikenellaceae_RC9_gut_group is the most abundant genus in the rumen bacterial community.

Key words: grass withering period, grazing Dongbao blackhead goats, ruminal environment, bacterial diversity, MiSeq sequencing

中图分类号:

S827

张年, 杨前平, 陶虎, 熊琪, 李晓锋, 陈明新, 索效军. 枯草期放牧东宝黑头羊瘤胃内环境参数及瘤胃细菌多样性分析[J]. 湖北农业科学, 2020, 59(23): 116-121.

ZHANG Nian, YANG Qian-ping, TAO Hu, XIONG Qi, LI Xiao-feng, CHEN Ming-xin, SUO Xiao-jun. Analysis on ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 116-121.

参考文献

[1] CLEMENTE J,URSELL L,PARFREY L,et al.The impact of the gut microbiota on human health:An integrative view[J].Cell,2012,148(6):1258-1270.
[2] 闵力,瞿明仁,戈婷婷,等.不同功能性寡糖组合对锦江黄牛瘤胃固相微生物多样性的影响[J].江西农业大学学报,2012,34(4):769-774.
[3] 徐琴.饲粮和品种对山羊瘤胃微生物多样性的影响[D].四川雅安:四川农业大学,2015.
[4] 马力,徐世晓,刘宏金,等.不同物候期牧草对放牧牦牛瘤胃内环境参数及瘤胃微生物多样性的影响[J].动物营养学报,2019,31(2):681-691.
[5] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37.
[6] 秦为琳.应用气相色谱测定瘤胃挥发性脂肪酸方法的研究改进[J].南京农学院学报,1982,5(4):110-116.
[7] NOCEK J E.Bobine acidosis:implications on laminitis[J].Journal of dairy science,1997,80(5):1005-1028.
[8] 张彩英,胡国良,曹华斌.反刍动物瘤胃内环境的特点及调控措施[J].中国畜牧兽医,2010,37(4):18-20.
[9] 杨宏波,刘红,占今舜,等.不同精粗比颗粒饲料对断奶公犊牛瘤胃发酵参数和微生物的影响[J].草业学报,2015,24(12):131-138.
[10] 尹召华,王梦芝,王洪荣,等.乙酸与丙酸比对体外瘤胃液挥发性脂肪酸发酵模式和微生物群体多样性的影响[J].动物营养学报,2011,23(12):2129-2135.
[11] BROSSARD L,MARTIN C,MICHALET-DOREAU B.Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep[J].Animal research,2003,52(6):513-530.
[12] 红敏.不同生长阶段天然牧草品质评定比较研究[J].饲料研究,2012(12):79-81.
[13] 陈芸,刘旗,邓俊良,等.复合抗菌肽对山羊瘤胃菌群结构的影响[J].浙江农业学报,2017,29(11):1800-1808.
[14] 陈芸,刘旗,邓俊良,等.基于MiSeq分析川中黑山羊瘤胃细菌的多样性及群落结构[J].湖南农业大学学报(自然科学版),2017,43(3):286-291.
[15] 范文斌,李长青,高仙灵,等.放牧蒙古羊瘤胃细菌随季节变化规律的研究[J].中国畜牧兽医,2019,46(8):2315-2321.
[16] 李烨青,奚雨萌,曾涵芳,等.基于16S rRNA测序分析奶牛体外发酵中瘤胃细菌多样性的变化[J].动物营养学报,2018,30(10):4059-4070.
[17] 李志鹏,刘晗璐,司华哲,等.不同粗饲料条件下梅花鹿瘤胃甲烷菌结构的比较分析[J].动物营养学报,2016,28(9):2911-2919.
[18] 张学燕,刘书杰,崔占鸿,等.应用16S rRNA基因序列技术分析青海高原放牧牦牛瘤胃产甲烷菌的多样性[J].青海大学学报,2018,36(1):9-16,46.
[19] ALEXADRE B,DE M,EVA L,et al.Microbiome analysis of dairy cows fed pasture or total mixed ration diets[J].Fems microbiology ecology,2011,78(2):256-265.
[20] 曾燕,简平,倪学勤,等.Illumina MiSeq测序平台测定蒙古羊瘤胃液相和固相菌群多样性[J].动物营养学报,2015,27(10):3256-3262.
[21] 彭科兰,任大喜,刘建新.不同年龄奶水牛瘤胃菌群多样性研究[J].动物营养学报,2019,31(11):5053-5064.
[22] 高雨飞,欧阳克蕙,瞿明仁,等.利用MiSeq测序技术分析锦江牛瘤胃细菌多样性[J].动物营养学报,2016,28(1):244-248.
[23] 李亚丹,任宏伟,吴彦彬,等.拟杆菌与肠道微生态[J].微生物学通报,2008,35(2):281-285.
[24] 董春晓,吕佳颖,牛骁麟,等.粗饲料来源对育肥湖羊瘤胃微生物区系及肌肉脂肪酸组成的影响[J].草业科学,2019,36(11):2926-2936.
[25] 张丽英.饲料分析及饲料质量检测技术[M].第四版.北京:中国农业大学出版社,2016.
[26] 李子健,李大彪,高民,等.不同生理阶段荷斯坦奶牛瘤胃细菌多样性研究[J].动物营养学报,2018,30(8):3017-3025.

枯草期放牧东宝黑头羊瘤胃内环境参数及瘤胃细菌多样性分析

Analysis on ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period

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