活性炭-聚四氟乙烯电极对生物电化学系统中NH4+-N去除的影响

doi:10.14088/j.cnki.issn0439-8114.2020.21.016

摘要/Abstract

摘要： 通过压力机将炭黑和聚四氟乙烯压缩,自制活性炭-聚四氟乙烯电极并置于单室生物电化学系统（BES）中,实现无外加有机碳源的自养型脱NH₄⁺-N。结果表明,通过给BES外加电压可以提升NH₄⁺-N的去除效果,并且随着外加电压从0.10 V提升至0.25 V,NH₄⁺-N的去除效果逐渐上升,平均去除率为47.8 mg/（L·d）。生物膜循环伏安（CV）分析结果显示,在电极的阳极和阴极存在多种氧化还原活性成分,说明用活性炭制成的生物阳极和生物阴极具有良好的电活性。基于高通量测序的16S rRNA基因的微生物群落分析表明,通电后微生物群落多样性明显增加,假单胞菌属（Pseudomonas）和副球菌属（Paracoccus）是BES中去除含氮化合物的重要功能菌群。

关键词: 活性炭, 生物电化学系统（BES）, 生物膜, NH₄⁺-N去除

Abstract: Carbon black and polytetrafluoroethylene （PTFE）were flat-pressed to fabricate the activated carbon （AC）-polytetrafluoroethylene electrodes. These AC-based electrodes were applied in a single-chamber BES to achieve autotrophic NH₄⁺-N removal under organic carbon-free conditions. The results showed that NH₄⁺-N removal effect could be enhanced by applying voltage to the BES, in which case the NH₄⁺-N removal efficiency gradually increased as the applied voltage increased from 0.10 V to 0.25 V. The average NH₄⁺-N removal rate in a BES was 47.8 mg/（L·d）. Cyclic voltammetry results revealed the presence of several redox-active components on the anode and cathode surfaces, indicating that the AC-based bioanode and biocathode had good electroactivities. Microbial community analysis of 16S rRNA genes based on high-through put sequencing indicated that the diversity of the microbial community increased after electric power application, and Pseudomonas and Paracoccus could be important for nitrogen-compound removal in the BES.

Key words: activated carbon, bioelectrochemical system （BES）, biofilm, NH₄⁺-N removal

中图分类号:

王睿, 张冬冬, 郭鹏, 王轶, 章春芳, 陈磊. 活性炭-聚四氟乙烯电极对生物电化学系统中NH₄⁺-N去除的影响[J]. 湖北农业科学, 2020, 59(21): 81-87.

WANG Rui, ZHANG Dong-dong, GUO Peng, WANG Yi, ZHANG Chun-fang, CHEN Lei. Effects of activated carbon-polytetrafluoroethylene electrodes on NH₄⁺-N removal in bioelectrochemical system[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(21): 81-87.

参考文献

[1] CHOI J H.Fabrication of a carbon electrode using activated carbon powder and application to the capacitive deionization process[J]. Separation and purification technology,2009,70(3):362-366.
[2] 杜新强,方敏,冶雪艳.地下水“三氮”污染来源及其识别方法研究进展[J].环境科学,2018,39(11):5266-5275.
[3] VANHULLES W H,VANDEWEYERH J P,MEESSCHAERTB D,et al.Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams[J]. Chemical engineering journal,2010,162(1):1-20.
[4] ZHANG F, CHENG S A,PANT D,et al.Power generation using an activated carbon and metal mesh cathode in a microbial fuel cell[J]. Electrochemistry communications,2009,11(11):2177-2179.
[5] ZOU J W, LU Y Y, HUANG Y.Estimates of synthetic fertilizer N-induced direct nitrous oxide emission from Chinese croplands during 1980—2000[J]. Environmental pollution,2009,158(2):631-635.
[6] FU Z M, ZHAO J.Impact of quinoline on activity and microbial culture of partial nitrification process[J]. Bioresource technology,2015,197:113-119.
[7] ZHANG Y,SHI Z, CHEN M X,et al.Evaluation of simultaneous nitrification and denitrification under controlled conditions by an aerobic denitrifier culture[J]. Bioresource technology,2015,175:602-605.
[8] VIRDIS B, RABAEY K, ROZENDAL R A,et al.Simultaneous nitrification denitrification and carbon removal in microbialfuel cells[J]. Water research,2010,44:2970-2980.
[9] ZHANG D D,YUAN X F,GUO P,et al.Microbial population dynamics and changes in main nutrients during the acidification process of pig manures[J]. Journal of environmental sciences,2011,23(3):497-505.
[10] ZHAN G Q,ZHANG L X,TAO Y,et al.Anodic ammonia oxidation to nitrogen gas catalyzed by mixed biofilms in bioelectrochemical systems[J]. Electrochimica acta,2014,135:345-350.
[11] EMIKO M, NOBUHIKO N, TOSHIAKI N K,et al.A simple screening procedure for heterotrophic nitrifying bacteria with oxygen-tolerant denitrification activity[J]. Journal of bioscience and bioengineering,2003,95(4):409-411.
[12] ASLAN S,DAHAB M.Nitritation and denitritation of ammonium-rich waste water using fluidized-bed biofilm reactors[J]. Journal of hazardous materials,2007,156(1):56-63.
[13] 叶星,马凯迪,黄俊生,等.反硝化生物滤池中生物膜量与脱氮效果和脱氢酶活性的关系[J].环境工程学报,2020,5(14):1210-1215.
[14] SHARMA M,JAIN P, VARANASI J L,et al.Enhanced performance of sulfate reducing bacteria based biocathode using stainless steel mesh on activated carbon fabric electrode[J]. Bioresource technology,2013,150:172-180.
[15] KHIN T, ANNACHHATRE A P.Novel microbial nitrogen removal processes[J].Biotechnology advances,2004,22(7):519-532.
[16] POIRIER S,QUÉMÉNER E D L,MADIGOU C,et al. Anaerobic digestion of biowaste under extreme ammonia concentration: Identification of key microbial phylotypes[J]. Bioresource technology,2016,207:92-101.
[17] ZHANG Q L,LIU Y,AI G M,et al.The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7[J]. Bioresource technology,2012,108:35-44.
[18] ROZENDAL R A, HAMELERS H V M, RABAEY K,et al. Towards practical implementation of bioelectrochemical wastewater treatment[J]. Trends in biotechnology,2008,26(8):450-459.
[19] 郑贤虹. 微生物电化学系统强化废水生物脱氮的工艺研究[D].杭州:浙江大学,2017.
[20] 丁阿强. 生物电极脱氮及其机理研究[D].杭州:浙江大学,2018.
[21] 吴金娜. 微生物燃料电池同步硝化反硝化脱氮性能及菌群分析[D].西安:长安大学,2017.
[22] ANNA V P, PUIG S,POUS N,et al.Microbiome characterization of MFCs used for the treatment of swine manure[J]. Journal of hazardous materials,2015,288:60-68.
[23] 王海曼. 微生物电化学系统强化含氨氮有机废水处理的效能与机制[D].哈尔滨:哈尔滨工业大学,2017.
[24] SUN Z B,LI Y J,WEI K J,et al.Simultaneous denitrification and carbon removal in microbial fuel cells[J].Asian agricultural research,2019,11(6):47-49.
[25] 尚菊红,宋美芹.基于MBBR工艺的污水处理厂生物脱氮除磷特征[J].中国给水排水,2019,35(15):100-105.
[26] 仇天雷. 循环水养殖废水生物脱氮技术及其影响因素研究[D].北京:北京化工大学,2016.
[27] HUGGINS T, WANG H M,KEARNS J,et al.Biochar as a sustainable electrode material for electricity production in microbial fuel cells[J]. Bioresource technology,2014,157:114-119.
[28] RUIZ G,JEISON D,CHAMY R.Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration[J]. Water research,2003,37(6):1371-1377.
[29] PAMBRUN V,PAUL E,SPERANDIO M.Modeling the partial nitrification in sequencing batch reactor for biomass adapted to high ammonia concentrations[J]. Biotechnology and bioengineering,2006,95(1):120-131.
[30] 王刚. 基于同时亚硝化/厌氧氨氧化/反硝化(SNAD)技术的污泥消化液脱氮工艺研究[D].辽宁大连:大连理工大学,2017.
[31] ZHAN G Q,ZHANG L X,LI D P,et al.Autotrophic nitrogen removal from ammonium at low applied voltage in a single-compartment microbial electrolysis cell[J]. Bioresource technology,2012,116:271-277.
[32] ZHANG W J,ZHANG Y,SU W T,et al.Effects of cathode potentials and nitrate concentrations on dissimilatory nitrate reductions by Pseudomonas alcaliphila in bioelectrochemical systems[J].Journal of environmental sciences,2014,26(4):885-891.
[33] SAKAKIBARA Y,NAKAYAMA T.A novel multi-electrode system for electrolytic and biological water treatments[J]. Water research,2001,35(3):768-778.
[34] WANG X,FENG C J,DING N,et al.Accelerated OH(-) transport in activated carbon air cathode by modification of quaternary ammonium for microbial fuel cells[J]. Environmental science & technology,2014,48(7):4191-4198.
[35] NAOKO Y,YASUSHI M,KASUMI D,et al.Graphene oxide-dependent growth and self-aggregation into a hydrogel complex of exoelectrogenic bacteria[J]. Scientific reports,2016,6:21867.
[36] SOROUSH S A,ABHIJEET S,MALTE H,et al.Effect of start-up strategies and electrode materials on carbon dioxide reduction on biocathodes[J]. Applied and environmental microbiology,2018,84(4):2-17.
[37] 吕鹏翼. 脱氮微生物的筛选及其在生物膜技术中的应用[D].北京:中国矿业大学(北京),2018.
[38] ZHANG D D,WANG Y,ZHANG C F,et al.Characterization of a thermophilic lignocellulose-degrading microbial consortium with high extracellular xylanase activity[J]. Journal of microbiology and biotechnology,2018,28(2):305-313.
[39] 臧华生. 微生物燃料电池对农田排水中氮磷去除效果的研究[D].北京:中国农业科学院,2019.
[40] NOTO K,OGASAWARA T,SUWA Y,et al.Complete oxidation of high concentration of ammonia by retaining incompatible nitrification activities in three-vessel system[J]. Water research,1998,32(3):769-773.
[41] DONG H,YU H B,WANG X,et al.A novel structure of scalable air-cathode without Nafion and Pt by rolling activated carbon and PTFE as catalyst layer in microbial fuel cells[J]. Water research,2012,46(17):5777-5787.
[42] SRINANDAN C S, SHAH M,PATEL B,et al.Assessment of denitrifying bacterial composition in activated sludge[J]. Bioresource technology,2011,102(20):9481-9489.
[43] 李辉耀,韩洪军.前置反硝化-BAF工艺反硝化池脱氮效能研究[J].水处理技术,2019,45(12):91-94.
[44] CHEN Q,NI J R.Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria[J]. Journal of industrial microbiology & biotechnology,2011,38(9):1305-1310.
[45] 邱雨婷,鲜思淑,朱晓华,等.生物强化技术在废水脱氮中的应用[J].生物技术世界,2015(2):10.
[46] ZHANG D D,LI Z L, ZHANG C F,et al.Phenol-degrading anode biofilm with high coulombic efficiency in graphite electrodes microbial fuel cell[J]. Journal of bioscience and bioengineering,2016,123(3):364-369.
[47] 蔡云波. 生物活性碳反应器强化生活污水同步硝化反硝化:C/N影响研究[J].环境科学与管理,2018,43(5):91-94.
[48] 胡正,林山杉,李天宇,等.反硝化菌生物阴极微生物燃料电池对模拟焦化废水的处理[J].西北师范大学学报(自然科学版),2018,54(6):58-63.
[49] 蒋柱武,颜丽红,张仲航,等.生物膜脱氮滤池的微生物群落结构特性[J].环境科学学报,2019,39(4):1148-1156.
[50] FUX C, VELTEN S, CAROZZI V,et al.Efficient and stable nitritation and denitritation of ammonium-rich sludge dewatering liquor using an SBR with continuous loading[J]. Water research,2006,40(14):2765-2775.
[51] MISHRA S S,MARKANDE A R,KELUSKAR R P,et al.Simultaneous nitrification and denitrification by novel heterotrophs in remediation of fish processing effluent[J]. Journal of basic microbiology,2015,55(6):772-779.
[52] 穆剑楠,吴朕君,杜亚飞,等.短程硝化SBR实时控制与微生物群落研究[J].工业水处理,2019,39(12):23-29.
[53] WANG X H,ZHENG Q,YUAN Y,et al.Bacterial community and molecular ecological network in response to Cr₂O₃ nanoparticles in activated sludge system[J]. Chemosphere,2017,188:10-17.
[54] SU W,ZHANG L,LI D,et al.Dissimilatory nitrate reduction by pseudomonas alcaliphila with an electrode as the sole electron donor[J]. Biotechnology and bioengineering,2012,109(11):2904-2910.
[55] KATO S, HASHIMOTO K, WATANABE K.Microbial interspecies electron transfer via electric currents through conductive minerals[J]. Proceedings of the national academy of sciences of the United States of America,2012,109:10042-10046.
[56] 温丹丹. 不同生物处理工艺活性污泥系统中微生物群落结构、代谢产物及与污泥沉降性能的关系研究[D].西安:西安建筑科技大学,2018.
[57] 高岩,周北海,孙晨,等.闲置厌氧氨氧化微生物的启动及微生物分析[J].环境保护科学,2017,43(5):43-50.
[58] ZHANG L L,SONG Y D,ZUO Y,et al.Integrated sulfur- and iron- based autotrophic denitrification process and microbial profiling in an anoxic fluidized-bed membrane bioreactor[J]. Chemosphere,2019,221:375-382.

活性炭-聚四氟乙烯电极对生物电化学系统中NH₄⁺-N去除的影响

Effects of activated carbon-polytetrafluoroethylene electrodes on NH₄⁺-N removal in bioelectrochemical system

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	任芸芸, 张莎, 裴挫萍, 张志林. 藻菌生物膜处理含镍废水及其胞外聚合物变化研究[J]. 湖北农业科学, 2023, 62(3): 135-140.
[2]	范凯轩, 钟永科. 椰壳活性炭改性后对百草枯吸附能力的影响[J]. 湖北农业科学, 2022, 61(4): 56-60.
[3]	范凯轩, 张鹃, 周巡, 刘雨瑶, 钟永科. 煤质活性炭改性对百草枯的去除探究[J]. 湖北农业科学, 2022, 61(3): 65-69.
[4]	范凯轩, 蒋帅, 李孟值, 李田田, 郝盼盼, 严湘, 杨纯金. 活性炭表面酸性基团对百草枯吸附的影响[J]. 湖北农业科学, 2022, 61(21): 67-72.
[5]	朱佳琪, 陈芳清, 黄永文, 熊丹伟, 刘杨赟. 活性炭对秸秆和猪粪混合厌氧发酵产生物甲烷的影响[J]. 湖北农业科学, 2022, 61(11): 44-49.
[6]	杨磊, 郭军, 王岩, 次仁多吉. 农林废弃物制备环境友好型缓释碳源生物膜载体材料[J]. 湖北农业科学, 2021, 60(18): 68-71.
[7]	薄晓玮, 杨志萍, 綦国红. 乌梅有机酸提取条件优化及其对生物膜形成的抑制作用[J]. 湖北农业科学, 2020, 59(13): 118-122.
[8]	陈天崖, 张世豪, 王亚茹, 邵蕾, 王仕鑫, 刘剑飞. 活性炭固定床吸附硝基苯废水性能研究[J]. 湖北农业科学, 2019, 58(8): 71-73.
[9]	卢琴, 罗青平, 张腾飞, 罗玲, 汪宏才, 王红琳, 温国元, 张蓉蓉, 张文婷, 邵华斌. 生物膜、毒力基因和耐药基因对APEC的致病性作[J]. 湖北农业科学, 2019, 58(23): 132-135.
[10]	綦国红, 巴云英, 杨志萍, 陈贵堂, 王海翔. 大蒜提取物对荧光假单胞菌生物膜形成的抑制作用[J]. 湖北农业科学, 2019, 58(23): 157-159.
[11]	张华俊, 刘敏, 陈海峰, 石敏球, 吴闪闪, 李志今, 乔毅. 黑臭河道生态修复工程人工水草处理效果研究[J]. 湖北农业科学, 2018, 57(15): 54-57.