[1] MCPHERRON A C, LEE S J.Double muscling in cattle due to mutations in the myostatin?gene[J]. Proceedings of the national academy of sciences of the United States of America,1997,94(23):12457-12461. [2] KAMBADUR R, SHARMA M, SMITH T P, et al.Mutations in Myostatin (GDF8) in Double-muscled belgian blue and piedmontese cattle[J]. Genome research, 1997, 7(9):910-916. [3] LEE S J, MCPHERRON A C.Myostatin and the control of skeletal muscle mass[J]. Current opinion in genetics & development, 1999, 9(5):604-607. [4] VAN LAERE A S, NGUYEN M, BRAUNSCHWEIG M, et al. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig[J]. Nature, 2003,425(6960):832-836. [5] DOUDNA J A, CHARPENTIER E.Genome editing: The new frontier of genome engineering with CRISPR-Cas9[J].Science, 2014, 346(6213): 1258096. [6] HSU P D,LANDER E S,ZHANG F.Development and applications of CRISPR-Cas9 for genome engineering[J]. Cell, 2014, 157(6): 1262-1278. [7] VALERIE K, POVIRK L F.Regulation and mechanisms of mammalian double-strand break repair[J]. Oncogene, 2003,22(37): 5792-5812. [8] SAN FILIPPO J, SUNG P, KLEIN H.Mechanism of eukaryotic homologous recombination[J]. Annu Rev Biochem,2008,77:229-257. [9] RAN F, HSU P, WRIGHT J, et al.Genome engineering using the CRISPR-Cas9 system[J]. Nat Protoc,2013,8:2281-2308. [10] 华文君, 毕延震, 刘西梅,等. CRISPR/Cas9技术制备猪肌肉生长抑制素基因敲除细胞[J]. 基因组学与应用生物学, 2015(5):52-56. [11] 綦世金,毕延震,刘西梅,等. 用Cre/LoxP和CRISPR基因工程技术构建猪肌肉生长抑制素双等位基因敲除细胞系[J]. 中国生物化学与分子生物学报,2017,33(10): 311-318. [12] GAUDELLI N M,KOMOR A C,REES H A, et al.Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage[J]. Nature, 2017, 551(7681):464-471. [13] ZUO E, SUN Y, WEI W, et al.Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos[J]. Science, 2019, 364(6437):289-292. [14] ANZALONE A V, RANDOLPH P B, DAVIS J R, et al.Search-and-replace genome editing without double-strand breaks or donor DNA[J]. Nature, 2019, 576(7785):149-157. |