SSIIIa基因在云南高抗性淀粉特种稻的遗传变异

doi:10.14088/j.cnki.issn0439-8114.2020.20.008

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (20): 35-38.doi: 10.14088/j.cnki.issn0439-8114.2020.20.008

SSIIIa基因在云南高抗性淀粉特种稻的遗传变异

李霞, 杜娟, 杨晓梦, 普晓英, 杨加珍, 曾亚文

云南省农业科学院生物技术与种质资源研究所,昆明 650205

收稿日期:2020-03-16 出版日期:2020-10-25 发布日期:2020-12-04
通讯作者: 曾亚文,男,二级研究员,博士,主要从事功能作物育种研究,(电子信箱)zengyw1967＠126.com。
作者简介:李霞(1981-),女,河南获嘉人,助理研究员,博士,主要从事功能作物分子育种研究,(电话)15398480628(电子信箱)lixia_napus＠163.com。

Genetic variation of SSIIIa gene in Yunnan special rice with high resistant starch

LI Xia, DU Juan, YANG Xiao-meng, PU Xiao-ying, YANG Jia-zhen, ZENG Ya-wen

Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China

Received:2020-03-16 Online:2020-10-25 Published:2020-12-04

摘要/Abstract

摘要： 对云南省高抗性淀粉特种稻品系及功米系列品种进行SSIIIa基因已报道剪接位点SNP检测,结果显示,云南省高抗性淀粉材料未出现已报道的剪接位点SNP突变。进一步对云南高抗性淀粉特种稻SSIIIa基因的序列进行遗传变异分析,共发现SNP遗传变异位点59个,插入缺失变异位点6个。其中,分布于基因编码区域的SNP位点18个,非同义变异12个。分析这些非同义变异位点在6份高抗性淀粉特种稻材料与云南省地方对照品种的差异,结果显示6份高抗性淀粉特种稻材料与对照品种的差异,主要为籼粳稻之间的差异,只有功米2号存在SNP特异性变异。

关键词: SSIIIa基因, 抗性淀粉, 水稻, 遗传变异

Abstract: The reported SNP splicing site of SSIIIa gene was detected on Yunnan special rice lines with high resistant starch and “Gongmi”series of varieties.The results showed that there was no reported splicing site SNP mutation in Yunnan high resistant starch materials.Furthermore,genetic variation analysis of SSIIIa gene sequence of Yunnan high-resistant starch special rice was conducted,and a total of 59 genetic variation sites of SNP were found,and 6 deletion/inserted mutation sites were detected.Among them,18 SNPs were distributed in the gene coding region,and 12 SNPs were non-synonymous variants.In addition,the differences of these non-homogenous variation loci between six high-resistant starch special rice materials and the local control varieties in Yunnan were analyzed.The results showed that the differences between six high-resistant starch special rice materials and the control varieties were mainly between indica and japonica rice,only Gongmi No.2 had snp-specific variation.

Key words: SSIIIa gene, resistant starch, rice, genetic variation

中图分类号:

S511

李霞, 杜娟, 杨晓梦, 普晓英, 杨加珍, 曾亚文. SSIIIa基因在云南高抗性淀粉特种稻的遗传变异[J]. 湖北农业科学, 2020, 59(20): 35-38.

LI Xia, DU Juan, YANG Xiao-meng, PU Xiao-ying, YANG Jia-zhen, ZENG Ya-wen. Genetic variation of SSIIIa gene in Yunnan special rice with high resistant starch[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(20): 35-38.

参考文献

[1]CHEN L,MAGLIADO J,ZIMMET P Z.The worldwide epidemiolo-gy of type 2 diabetes mellitus-present and future perspectives[J].Nat Rev Endocrinol,2011,8:228-236.
[2]RAIGOND P,EZEKIEL R,RAIGOND B.Resistant starch in food:A review[J].J Sci Food Agric,2015,95(10):1968-1978.
[3]YAMADA Y,HOSOYA S,NISHIMURA S,et al.Effect of bread containing resistant starch on postprandial blood glucose levels in humans[J].Bioscience,biotechnology and biochemistry,2005,69:559-566.
[4]BOERS H M,SEIJEN T H J,MELA D J.A systematic review of the influence of rice characteristics and processing methods on post-prandial glycaemic and insulinaemic responses[J].British journal of nutrition,2015,114:1035-1045.
[5]KAUR B,RANAWANA V,HENRY J.The glycemic index of rice and rice products:A review,and table of GI values[J].Critical re-views in food science and nutrition,2016,56:215-236
[6]罗曦,黄锦峰,朱永生,等.水稻功米3号高抗性淀粉性状的遗传分析[J].农业生物技术学报,2014,22(1):10-16.
[7]孙志敏,白建江,朱辉明,等.混配比例及加工方式对“降糖稻1号”稻米产品抗性淀粉含量的影响[J].核农学报,2012,26(2):318-323.
[8]杨朝柱,李春寿,舒小丽,等.富含抗性淀粉水稻突变体的淀粉特性[J].中国水稻科学,2005(19):516-520.
[9]ZHOU H,WANG L,LIU G,et al.Critical roles of soluble starch synthase SSIIIa and granule-bound starch synthase Waxy in synthe-sizing resistant starch in rice[J].Proc Natl Acad Sci,2016,113:12844-12849.
[10]KHARABIAN-MASOULEH A,WATERS D L,REINKE R F,et al.Discovery of polymorphisms in starch-related genes in rice germ-plasm by amplification of pooled DNA and deeply parallel sequenc-ing[J].Plant Biotechnol J,2011,9(9):1074-1085.
[11]李颜方,王高鸿,杜艳伟,等.作物抗性淀粉研究进展[J].中国农业科技导报,2019(21):56-62.
[12]YANG R F,SUN C L,BAI J J,et al.A putative gene sbe3-rs for resistant starch mutated from SBE3 for starch branching enzyme in rice(Oryza sativa L.)[J].PLoS One,2012,7(8):e43026.
[13]CAI J,MAN J,HUANG J,et al.Relationship between structure and functional properties of normal rice starches with different am-ylose contents[J].Carbohydrate polymers,2015,125:35-44.
[14]LIN L,GUO D,HUANG J,et al.Molecular structure and enzymat-ic hydrolysis properties of starches from high-amylose maize in-bred lines and their hybrids[J].Food hydrocolloids,2016,58:246-254.
[15]CROFTS N,ABE K,AIHARA S,et al.Lack of starch synthase IIIa and high expression of granule-bound starch synthase I synergisti-cally increase the apparent amylose content in rice endosperm[J].Plant science,2012,193-194:62-69.
[16]王自布,黄燕芬,吴坤,等.籽粒淀粉合成酶与淀粉合成关系的研究进展[J].生物技术进展,2013(3):336-341.
[17]JEON J S,RYOO N,HAHN T R,et al.Starch biosynthesis in ce-real endosperm[J].Plant physiology and biochemistry,2010,48:383-392.
[18]KHARABIAN-MASOULEH A,WATERS D L E,REINKE R F,et al.SNP in starch biosynthesis genes associated with nutritional and functional properties of rice[J].Scientific reports,2012,2:557.

SSIIIa基因在云南高抗性淀粉特种稻的遗传变异

Genetic variation of SSIIIa gene in Yunnan special rice with high resistant starch

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郝玲, 杨莹, 张佩, 邱航, 钱沈旸. 基于大气环流特征量和海温的水稻产量结构因素预报模型研究——以江苏省为例[J]. 湖北农业科学, 2023, 62(8): 27-36.
[2]	肖婉钰, 孙艺嘉, 周贤玉, 任海龙, 张晶, 黄江华, 许东林. 水稻发芽率测定中污染性微生物的鉴定及多样性[J]. 湖北农业科学, 2023, 62(7): 51-56.
[3]	邓钊, 王凯, 杜波, 祝莉莉, 杨远柱, 陈荣智. 水稻抗褐飞虱基因Bph43紧密连锁KASP标记开发与验证[J]. 湖北农业科学, 2023, 62(6): 169-174.
[4]	李兴华, 陈展鹏, 蔡正军, 邹彩琼, 张中南, 张文超, 丁凤菊. 稻田套养不同密度黑斑蛙对水稻产量和土壤肥力的影响初探[J]. 湖北农业科学, 2023, 62(5): 5-7.
[5]	刘春萍, 徐孜, 龚洪波, 金兴国, 魏静, 刘婧, 张拥军, 邱东峰. 水稻稻曲病研究进展[J]. 湖北农业科学, 2023, 62(4): 89-92.
[6]	熊又升, 熊汉锋, 谢媛园, 樊红霞, 徐小羽, 刘喻, 周剑雄, 刘威, 王时秋. 新型肥料在稻-油轮作制中的施用效果[J]. 湖北农业科学, 2023, 62(3): 69-74.
[7]	王璟, 陈碧珊, 叶林海, 卢芷晴, 钟诗明, 李炎松, 李烨, 冯婷婷, 宋军霞. 南渡河沿岸水稻田土壤有机氯农药污染及健康风险评价[J]. 湖北农业科学, 2023, 62(3): 149-156.
[8]	徐芬, 郭红岩, 喻小兵, 彭立军, 姚晶晶. CO₂浓度升高和有机肥-化肥施用对水稻光合作用、抗氧化酶活性和重金属含量的影响[J]. 湖北农业科学, 2023, 62(12): 51-55.
[9]	黎雨薇, 黄志谋, 杨汉, 瞿和平, 全文博, 秦晓银, 李忠正. 栽培方式对不同品种水稻产量和经济效益的影响[J]. 湖北农业科学, 2023, 62(11): 5-10.
[10]	邱儒琼, 彭少坤, 李孟璠. 基于深度学习和遥感数据的水稻种植面积提取[J]. 湖北农业科学, 2023, 62(11): 176-182.
[11]	童彤, 魏君英, 何蒲明. 加入农民专业合作社对水稻种植户绿色生产意愿的影响——基于江汉平原的调研[J]. 湖北农业科学, 2023, 62(11): 227-232.
[12]	胡继红, 柯传勇, 刘善德, 黄海燕, 廖光毅. 杂交水稻籼型三系不育系珈丰91A的选育及应用[J]. 湖北农业科学, 2023, 62(10): 9-12.
[13]	邵远刚, 刘红菊, 孙勇, 贾顺超, 范玉莲. 有机无机复合肥对水稻施用效果及适用量试验初报[J]. 湖北农业科学, 2022, 61(9): 13-15.
[14]	胡菊芳, 占龙飞, 谢远玉. 江西省近60年极端高温变化特征及其对水稻的影响[J]. 湖北农业科学, 2022, 61(9): 27-32.
[15]	刘笑, 周晶, 郭张薇, 马梦燕. 关系网络对农机本地作业服务市场的影响研究——以湖北省监利市和江陵县为例[J]. 湖北农业科学, 2022, 61(9): 197-202.