QuEChERS-气相色谱法快速测定蔬菜中的咪鲜胺及2,4,6-三氯苯酚残留量

doi:10.14088/j.cnki.issn0439-8114.2019.01.025

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (1): 103-106.doi: 10.14088/j.cnki.issn0439-8114.2019.01.025

QuEChERS-气相色谱法快速测定蔬菜中的咪鲜胺及2,4,6-三氯苯酚残留量

张彦军^{1, 2}, 钱滢文^{1, 2}, 柴宗龙^{1, 2}, 石瑞瑞^{1, 2}, 张楠^{1, 2}, 尤毅娜^{1, 2}, 刘琦^{1, 2}, 洪霞^{1, 2}

1.甘肃省商业科技研究所有限公司,兰州 730000;
2.甘肃中商食品质量检验检测有限公司,兰州 730010

收稿日期:2018-06-13 出版日期:2019-01-10 发布日期:2019-11-26
作者简介:张彦军（1987-）,男,甘肃礼县人,工程师,硕士,主要从事碳纳米材料在食品检测方面的工作,（电话）18189535998（电子信箱）zyj198971@163.com。

Rapid Determination of Residues of Prochloraz and 2,4,6-Trichlorophenol in Vegetables by QuEChERS-Gas Chromatogarphy

ZHANG Yan-jun^{1, 2}, QIAN Ying-wen^{1, 2}, CHAI Zong-long^{1, 2}, SHI Rui-rui^{1, 2}, ZHANG Nan^{1, 2}, YOU Yi-na^{1, 2}, LIU Qi^{1, 2}, HONG Xia^{1, 2}

1.Gansu Institute of Business and Technology Co.,Ltd.,Lanzhou 730010,China;
2.Gansu Zhongshang Food Quality Test and Detection Co.,Ltd.,Lanzhou 730010,China

Received:2018-06-13 Online:2019-01-10 Published:2019-11-26

摘要/Abstract

摘要： 建立了QuEChERS-气相色谱快速测定蔬菜中咪鲜胺及其代谢物2,4,6-三氯苯酚残留量的方法。样品经乙酸酸化乙腈提取后,以无水硫酸镁和N-丙基乙二胺（PSA）净化,并利用气相色谱进行检测。结果表明,在最佳条件下,咪鲜胺及其代谢物2,4,6-三氯苯酚分别在0.005~1.000和0.002~1.000 μg/mL范围内线性关系良好,相关系数分别为0.998 6和0.999 3,检出限分别为0.001 0和0.000 6 mg/kg;在0.02、0.10、0.50 μg/mL 3个添加水平下回收率分别为92.1%~107.9%和91.4%~105.7%,相对标准偏差分别为2.4%~10.4%和2.6%~11.3%。该方法具有快速、便捷、安全、清洁和成本低等特点,可用于蔬菜中咪鲜胺及其代谢物2,4,6-三氯苯酚残留的快速测定。

关键词: QuEChERS, 气相色谱, 咪鲜胺, 2, 4, 6-三氯苯酚, 蔬菜

Abstract: To developed a rapid method for the determination of residues of prochloraz and its metabolite 2,4,6-trichlorophenol in vegetables by QuEChERS and gas chromatography（GC）. The samples were extracted with acetonitrile-acetic acid, purified by PSA,and determined by GC. The results showed that prochloraz and its metabolite 2,4,6-trichlorophenol had a good linearity in the range of 0.005~1.000 μg/mL and 0.002~1.000 μg/mL, the correlation were 0.998 6 and 0.999 3 under the optimal conditions. The limit of detection for prochloraz and its metabolite 2,4,6-trichlorophenol were 0.001 0 mg/kg and 0.000 6 mg/kg, respectively. Their average recoveries at spiked levels of 0.01, 0.10 and 0.50 μg/mL were 92.1%~107.9% and 91.4%~105.7% with relative standard deviations of 2.4%~10.4% and 2.6%~11.3%, respectively. This method was simple, efficient, quick, convenient, safe, clean, low-cost, and can be suitable for the analysis of prochloraz and 2,4,6-trichlorophenol residues in fruit vegetables.

Key words: QuEChERS, gas chromatography, prochloraz, 2, 4, 6-trichlorophenol, vegetables

中图分类号:

O657.63

张彦军, 钱滢文, 柴宗龙, 石瑞瑞, 张楠, 尤毅娜, 刘琦, 洪霞. QuEChERS-气相色谱法快速测定蔬菜中的咪鲜胺及2,4,6-三氯苯酚残留量[J]. 湖北农业科学, 2019, 58(1): 103-106.

ZHANG Yan-jun, QIAN Ying-wen, CHAI Zong-long, SHI Rui-rui, ZHANG Nan, YOU Yi-na, LIU Qi, HONG Xia. Rapid Determination of Residues of Prochloraz and 2,4,6-Trichlorophenol in Vegetables by QuEChERS-Gas Chromatogarphy[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(1): 103-106.

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QuEChERS-气相色谱法快速测定蔬菜中的咪鲜胺及2,4,6-三氯苯酚残留量

Rapid Determination of Residues of Prochloraz and 2,4,6-Trichlorophenol in Vegetables by QuEChERS-Gas Chromatogarphy

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