湖北农业科学 ›› 2020, Vol. 59 ›› Issue (17): 107-111.doi: 10.14088/j.cnki.issn0439-8114.2020.17.025

• 贮藏·加工 • 上一篇    下一篇

三种荚蒾中香豆素提取工艺及含量比较分析研究

牛茵茵, 廖腾飞, 王丽, 孙敏, 徐平, 罗兴武   

  1. 湖北民族大学生物科学与技术学院,湖北 恩施 445000
  • 收稿日期:2019-10-20 发布日期:2020-10-23
  • 通讯作者: 罗兴武(1975-),男,副教授,硕士,主要从事天然产物开发与利用研究,(电话)18694011377(电子信箱)lxwmss@163.com。
  • 作者简介:牛茵茵(1996-),女,山西晋城人,在读硕士研究生,研究方向为食品科学与工程,(电话)18435993461(电子信箱)18435993461@163.com。
  • 基金资助:
    2018年湖北省大学生创新创业训练计划项目(201810517043)

Study on extraction technology and content of coumarin from three varieties of Viburnum

NIU Yin-yin, LIAO Teng-fei, WANG Li, SUN Min, XU Ping, LUO Xing-wu   

  1. College of Biological Science and Technology, Hubei Minzu University, Enshi 445000, Hubei, China
  • Received:2019-10-20 Published:2020-10-23

摘要: 以皱叶荚蒾(Viburnum rhytidophyllum)、球核荚蒾(Viburnum propinquum)和鸡树条荚蒾(Viburnum sargentii)为原料,选取乙醇浓度、提取时间、提取温度和料液比为因素,吸光度为指标进行单因素试验,选取适当因素水平进行正交试验,并采用高效液相色谱法(HPLC)进行试验,采用优化后的工艺进行提取,比较3种荚蒾(Viburnum dilatatum)中香豆素的提取率。结果表明,3种荚蒾中均含有香豆素,其中,皱叶荚蒾中香豆素的含量最高,球核荚蒾次之,鸡树条荚蒾最少;皱叶荚蒾和球核荚蒾香豆素最佳提取条件为乙醇浓度55%、提取时间60 min、提取温度80 ℃、料液比1∶25(g∶mL);鸡树条荚蒾香豆素最佳提取条件为乙醇浓度55%、提取时间80 min、提取温度70 ℃、料液比1∶25(g∶mL)。不同品种荚蒾中香豆素的提取工艺和所提取的香豆素含量均存在差异。

关键词: 荚蒾(Viburnum dilatatum), 香豆素, 提取优化, 测定比较

Abstract: The raw materials are Viburnum rhytidophyllum,Viburnum propinquum and Viburnum sargentii. The single factor experiment was carried out by selecting ethanol concentration, extraction time, extraction temperature and ratio of material to liquid as factors, absorbance as index, and then orthogonal test analysis was carried out at appropriate factor level and HPLC was used to carry out the experiment. The yield of coumarin from three varieties of Viburnum was compared by the optimized extraction process. The results showed that coumarin was found in all three kinds of Viburnum, Viburnum rhytidophyllum was the highest,Viburnum propinquum was the second, and Viburnum sargentii was the least. The optimum conditions of Viburnum rhytidophyllum and Viburnum propinquum were as followsThe concentration of ethanol was 55%, the extraction time was 60 min, the extraction temperature was 80 ℃, and the ratio of material to liquid was 1∶25. The optimum conditions of Viburnum sargentii were as followsThe concentration of ethanol was 55%, the extraction time was 80 min, the extraction temperature was 70 ℃, and the ratio of material to liquid was 1∶25. The content of coumarin was different in the extraction process of different varieties of Viburnum.

Key words: Viburnum dilatatum, coumarin, extraction optimization, comparison of measurements

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