吡格列酮对猪卵母细胞体外成熟及孤雌胚胎发育的影响

doi:10.14088/j.cnki.issn0439-8114.2020.17.022

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (17): 93-96.doi: 10.14088/j.cnki.issn0439-8114.2020.17.022

吡格列酮对猪卵母细胞体外成熟及孤雌胚胎发育的影响

吕玲燕¹, 汪燕玲¹, 潘星辰², 张家庆³, 吴永绍¹, 孙如玉¹, 潘天彪¹, 张冰¹

1.广西壮族自治区畜牧研究所/广西家畜遗传改良重点实验室,南宁 530001;
2.广西柯莉莱原种猪有限责任公司,南宁 530001;
3.河南省农业科学院畜牧兽医研究所,郑州 450002

收稿日期:2019-10-21 发布日期:2020-10-23
通讯作者: 张冰,高级畜牧师,主要从事动物育种及种质资源保护工作的研究,（电话）13471156371（电子信箱）zbcherry@126.com。
作者简介:吕玲燕（1985-）,女,广西桂林人,助理研究员,硕士,主要从事动物胚胎工程和分子遗传育种研究, （电话）13481192047（电子信箱）llyan159@163.com。
基金资助:
桂渔牧科（201452007）; 桂牧研科（2019-02）; 广西科技重大专项（AA17204052）

Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig

LYU Ling-yan¹, WANG Yan-ling¹, PAN Xing-chen², ZHANG Jia-qing³, WU Yong-shao¹, SUN Ru-yu¹, PAN Tian-biao¹, ZHANG Bing¹

1. Guangxi Key Laboratory of Livestock Genetic Improvement/Guangxi Institute of Animal Sciences, Nanning 530001, China;
2. Guangxi Kelilai Original Breeding Pig Limited Liability Company, Nanning 530001,China;
3. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received:2019-10-21 Published:2020-10-23

摘要/Abstract

摘要： 为探讨吡格列酮（PIO）对猪卵母细胞体外成熟及孤雌胚胎发育的影响,比较了不同浓度PIO对卵母细胞的体外成熟状况以及孤雌胚胎体外发育潜能的影响。结果表明,成熟培养液中添加1.0 μmol/L PIO组的极体排出率显著高于0.5 μmol/L添加组（P<0.05）,极显著高于对照和1.5 μmol/L添加组（P<0.01）。对在4种PIO浓度中培养成熟的卵母细胞进行孤雌激活,结果表明,添加1.0 μmol/L PIO组的卵裂率显著高于0.5 μmol/L添加组（P<0.05）,极显著高于对照和1.5 μmol/L添加组（P<0.01）;就囊胚率及细胞总数而言,1.0 μmol/L PIO添加组极显著高于对照、0.5、1.5 μmol/ L这3个PIO添加组（P<0.01）,表明成熟培养液中添加1.0 μmol/L PIO有助于提高猪卵母细胞的成熟率,以及孤雌胚胎的囊胚率和囊胚细胞总数。

关键词: 吡格列酮, 猪, 卵母细胞, 孤雌胚胎, 发育效果

Abstract: Aiming to investigate the effects of pioglitazone（PIO） on oocytesmaturation in vitro and parthenogenetic embryos development of pig, the effect of different concentration PIO on oocytes maturation in vitro and parthenogenetic embryos developmental potency were compared. The results showed that the polar body excretion rate of 1.0 μmol/L PIO-treated group was significantly higher than that of 0.5 μmol/L PIO-treated group in maturation medium（P<0.05）,was very significantly higher that of control an 1.5 μmol/L PIO-treated group（P<0.01）. The mature oocytes cultured in different concentrations PIO were parthenogenetic activated, the experimental results showed that the cleavage rate of the 1.0 μmol/L PIO group was significantly higher than of the 0.5 μmol/L group in embryo culture medium（P<0.05）, was very significantly higher than that of the control and 1.5 μmol/L PIO group （P<0.01）, as for the blastocyst rate and the total number of cells, the group of the 1.0 μmol/L PIO-treated group was the highest among the groups （P<0.01）. In conclusion, 1.0 μmol/L PIO in maturation medium could improve the maturation rate of porcine oocyte, also could significantly improve the blastocyst rate and the total number of cells of porcine parthenogenetic embryos.

Key words: pioglitazone, pig, oocytes, parthenogenetic embryos, developmental effect

中图分类号:

S828
Q954.4

吕玲燕, 汪燕玲, 潘星辰, 张家庆, 吴永绍, 孙如玉, 潘天彪, 张冰. 吡格列酮对猪卵母细胞体外成熟及孤雌胚胎发育的影响[J]. 湖北农业科学, 2020, 59(17): 93-96.

LYU Ling-yan, WANG Yan-ling, PAN Xing-chen, ZHANG Jia-qing, WU Yong-shao, SUN Ru-yu, PAN Tian-biao, ZHANG Bing. Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 93-96.

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吡格列酮对猪卵母细胞体外成熟及孤雌胚胎发育的影响

Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig

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