应用Illumina MiSeq高通量测序技术分析堇叶碎米荠根际土壤微生物多样性

doi:10.14088/j.cnki.issn0439-8114.2020.17.013

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (17): 58-62.doi: 10.14088/j.cnki.issn0439-8114.2020.17.013

应用Illumina MiSeq高通量测序技术分析堇叶碎米荠根际土壤微生物多样性

陈菲菲^1,2, 丛欣³, 向极钎^1,2, 李红英^1,2, 孙举志^1,2, 许锋⁴, 薛华⁵

1.恩施土家族苗族自治州农业科学院,湖北恩施 445000;
2.湖北省富硒产业技术研究院,湖北恩施 445000;
3.恩施德源健康科技发展有限公司,湖北恩施 445000;
4.武汉轻工大学,武汉 430023;
5.国家富硒产品质量监督检验中心（湖北）,湖北恩施 430000

收稿日期:2019-11-25 发布日期:2020-10-23
通讯作者: 向极钎（1965-）,男,正高职农艺师,（电子信箱）2416861638@qq.com。
作者简介:陈菲菲（1988-）,女,湖北恩施人,助理研究员,硕士,主要从事食品及药用微生物研究,（电话）18271717164（电子信箱）672394220@qq.com。
基金资助:
国家自然科学基金项目（31560579）; 恩施州科技计划项目（E20180005）

Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system

CHEN Fei-fei^1,2, CONG Xing³, XIANG Ji-qian^1,2, LI Hong-ying^1,2, SUN Ju-zhi^1,2, XU Feng⁴, XUE Hua⁵

1. Agricultural Sciences Academy of Enshi Tujia and Miao Automous Prefecture,Enshi 445000, Hubei, China;
2. Hubei Selenium Industry Technology Research Institute, Enshi 445000, Hubei, China;
3. Enshi Deyuan Health Technology Co.,Ltd.,Enshi 445000, Hubei, China;
4. Wuhan Polytechnic University, Wuhan 430023, China;
5. National Center of Supervision and Inspection of Selenium Enriched Products, Enshi 445000, Hubei, China

Received:2019-11-25 Published:2020-10-23

摘要/Abstract

摘要： 以生长野生堇叶碎米荠（Cardamine violifolia O. E Schulz）的3个地区（湖北省恩施市双河镇、湖北省宜昌市五峰县、湖南省常德市石门县）的9个根际土壤样品为材料,应用 Illumina MiSeq 高通量测序技术分析其微生物群落的多样性。结果显示,来自恩施市双河镇土样S1与来自宜昌市五峰县土样S2微生物类群的一致性最高,差异较小,S1与来自常德市石门县土样S3、S2与S3土样微生物类群一致性较低。多样性指数和稀释性曲线分析得出,在9个土壤样品中,来自恩施市双河镇土样的S1.2号Shannon指数最大,Simpson指数最小;来自常德市石门县土样的S3.2号Shannon指数最小,Simpson指数最大,说明恩施市双河镇土样的群落多样性最高,常德市石门县土样的群落多样性最低。3个地区土样中占优势的菌门均为变形菌门（Proteobacteria）;共有的菌门有泉古菌门（Crenarchaeota）、酸杆菌门（Acidobacteria）、放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）、衣原体门（Chlamydiae）、绿弯菌门（Chloroflexi）、厚壁菌门（Firmicutes）、芽单胞菌门（Gemmatimonadetes）、硝化螺旋菌门（Nitrospirae）、OD1、浮霉菌门（Planctomycetes）、变形菌门、疣微菌门（Verrucomicrobia）和WS3。

关键词: 堇叶碎米荠（Cardamine violifolia O. E Schulz）, Illumina MiSeq 高通量测序, 根际土壤, 微生物多样性

Abstract: Taking nine soil samples growing Cardamine violifolia O. E Schulz from three area （Shuanghe town, Enshi city; Wufeng county, Yichang city, Hubei province and Shimen county, Changde city, Hunan province） as materials, the microbial community structure and diversity were analyzed by using Illumina MiSeq high-throughput sequencing system. The results showed that the microbial community composition of S1 soil sample form Shuanghe and S2 soil sample from Wufeng were similar, but the composition were quite different between S1 soil sample and S3 soil sample from Shimen, and were different between S2 and S3 soil sample. The diversity index and rarefaction curves analysis showed that in nine soil samples, the highest microbial diversity was No. S1.2 soil sample from Shuanghe and the lowest microbial diversity was No. S3.2 soil sample from Shimen. It indicated that the community diversity of Shuanghe soil sample was the highest and that of Shimen soil sample was the lowest. Proteobacteria was the dominant population in three area soil. The same phyla of the three area were Crenarchaeota, Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Firmicutes, Gemmatimonadetes, Nitrospirae, OD1, Planctomycetes, Proteobacteria, Verrucomicrobia and WS3.

Key words: Cardamine violifolia O. E Schulz, Illumina MiSeq high-throughput sequencing, rizosphere soil, microbial diversity

中图分类号:

Q948.15
Q938

陈菲菲, 丛欣, 向极钎, 李红英, 孙举志, 许锋, 薛华. 应用Illumina MiSeq高通量测序技术分析堇叶碎米荠根际土壤微生物多样性[J]. 湖北农业科学, 2020, 59(17): 58-62.

CHEN Fei-fei, CONG Xing, XIANG Ji-qian, LI Hong-ying, SUN Ju-zhi, XU Feng, XUE Hua. Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 58-62.

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应用Illumina MiSeq高通量测序技术分析堇叶碎米荠根际土壤微生物多样性

Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system

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