一株耐盐性α-淀粉酶高产菌的分离、鉴定及诱变选育

doi:10.14088/j.cnki.issn0439-8114.2020.16.029

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (16): 130-133.doi: 10.14088/j.cnki.issn0439-8114.2020.16.029

一株耐盐性α-淀粉酶高产菌的分离、鉴定及诱变选育

张伟¹, 吴晓卫², 姚彦红¹, 张芬芬¹, 周晓伦¹

1.甘肃医学院病原生物学教研室,甘肃平凉 744000;
2.深圳市尚维高科有限公司分子生物学研究室,广东深圳 518000

收稿日期:2019-11-10 出版日期:2020-08-25 发布日期:2020-10-09
通讯作者: 周晓伦,讲师,主要从事土壤微生物研究,（电子信箱）610253095@qq.com。
作者简介:张伟（1984-）,男,甘肃庆阳人,讲师,主要从事微生物物种资源研究,（电话）18293373738（电子信箱）274591224@qq.com
基金资助:
甘肃省高等学校创新能力提升项目（2019B-203）

Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity

ZHANG Wei¹, WU Xiao-wei², YAO Yan-hong¹, ZHANG Fen-fen¹, ZHOU Xiao-lun¹

1. Department of Pathogenic Biology, Gansu Medical College, Pingliang 744000, Gansu, China;
2. Molecular Biology Lab, Shenzhen Shangwei High Technology Co., Ltd., Shenzhen 518000, Guangdong, China

Received:2019-11-10 Online:2020-08-25 Published:2020-10-09

摘要/Abstract

摘要： 从土壤中分离筛选出一株产α-淀粉酶的耐盐性菌株NWU-8,通过形态学、生理生化和16S rRNA基因序列分析,将NWU-8菌株鉴定为Halorubrum sp.,初始淀粉酶活力为32 U/mL。为提高该菌株产α-淀粉酶的能力,采用He-Ne激光照射手段对其进行诱变育种,所获突变株NWU-8-14产α-淀粉酶活力达到138 U/mL,产酶活力提升331%。此外,在盐浓度高达35%时,其α-淀粉酶活力仍然保持80 U/mL。NWU-8-14作为一种高产淀粉酶的噬盐菌在食品发酵及环境保护方面有广阔的应用前景。

关键词: 淀粉酶, 嗜盐菌, 诱变, 酶活力

Abstract: A halophilic strain NWU-8 that produced α-amylase was isolated and screened from the soil. Through morphological, physiological and biochemical and 16S rRNA gene sequence analysis, the strain NWU-8 was identified as Halorubrum sp., and the initial amylase activity was 32 U/mL. In order to improve the ability of the strain to produce α-amylase, the strain NWU-8 was mutated by He-Ne irradiation. After the mutation, the mutant NWU-8-14 produced alpha-amylase activity up to 138 U/mL, which was 331% higher than the wild strain. In addition, when the salt concentration was as high as 35%, its α-amylase activity still maintained 80 U/mL. NWU-8-14 was a kind of salt phages with high amylase production. This mutant strain had broad application prospects in food fermentation and environmental protection.

Key words: amylase, halophilic bacteria, mutation, enzyme activity

中图分类号:

TS201

张伟, 吴晓卫, 姚彦红, 张芬芬, 周晓伦. 一株耐盐性α-淀粉酶高产菌的分离、鉴定及诱变选育[J]. 湖北农业科学, 2020, 59(16): 130-133.

ZHANG Wei, WU Xiao-wei, YAO Yan-hong, ZHANG Fen-fen, ZHOU Xiao-lun. Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(16): 130-133.

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一株耐盐性α-淀粉酶高产菌的分离、鉴定及诱变选育

Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity

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