鸡舍空气中葡萄球菌的分离鉴定及耐药性分析

doi:10.14088/j.cnki.issn0439-8114.2020.16.026

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (16): 118-121.doi: 10.14088/j.cnki.issn0439-8114.2020.16.026

鸡舍空气中葡萄球菌的分离鉴定及耐药性分析

卢苑青^1,2, 张腾飞², 邵华斌², 曾驰¹

1.武汉轻工大学生物与制药工程学院,武汉 430023;
2.湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室,武汉 430064

收稿日期:2020-01-14 出版日期:2020-08-25 发布日期:2020-10-09
通讯作者: 张腾飞,副研究员,（电子信箱）tfzhang23@163.com。
作者简介:卢苑青（1994-）,女,山东东营人,在读硕士研究生,研究方向为禽细菌病的流行病学,（电话）17671647921（电子信箱）wanylu@outlook.com
基金资助:
现代农业产业技术体系资助项目（nycytx-41-G13）

Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses

LU Yuan-qing^1,2, ZHANG Teng-fei², SHAO Hua-bin², ZENG Chi¹

1. School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University, Wuhan 430023, China;
2. Institute of Animal Husbandry and Veterinary/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received:2020-01-14 Online:2020-08-25 Published:2020-10-09

摘要/Abstract

摘要： 凝固酶阴性葡萄球菌是广泛存在于自然界中的一种革兰氏阳性条件致病菌,畜禽养殖舍是其病原体传播的一个重要传染源,但其在家禽舍气溶胶中的流行情况和特征并不明确。通过对从家禽养殖舍生物气溶胶采集而来的细菌进一步分离鉴定,共获得了102株凝固酶阴性葡萄球菌（coagulase-negative staphylococci,CoNS）,其中松鼠葡萄球菌（41.18%）是最主要的菌种,此外还包括马胃葡萄球菌（21.57%）、腐生葡萄球菌（18.63%）、溶血葡萄球菌（10.78%）、木糖葡萄球菌（5.88%）等。耐药性分析结果显示,分离的葡萄球菌对青霉素、氨苄西林、环丙沙星、氯霉素、红霉素、四环素、克林霉素和苯唑西林均呈现高耐药率。PCR检测结果显示,有36.3%的菌株携带有耐药基因mecA,且均对苯唑西林耐药,而mecA是耐甲氧西林金黄色葡萄球菌（MRSA）的标志耐药基因。

关键词: 家禽舍, 气溶胶, 凝固酶阴性葡萄球菌（coagulase-negative staphylococci, CoNS）, 耐药性, mecA

Abstract: Coagulase-negative staphylococci （CoNS） are a group of opportunistic pathogens, which are widely spread in the environment. Animal breeding is an important source of pathogen spreading. However, the prevalence and characteristics of CoNS in the bioaerosols of poultry houses are unclear. In this study, through further isolation and identification of bacteria collected from bioaerosols in poultry farms, 102 strains of coagulase-negative staphylococci were collected. Among these isolates, 41.18% isolates were Staphylococcus sciuri, which was the predominant species, followed by S. equorum （21.57%）, S. saprophyticus （18.63%）, S. haemolyticus （10.78%） and S. xylosus （5.88%）. Resistance analysis showed that high resistance rates to penicillin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, tetracycline, clindamycin and oxacillin among these staphylococci. The presence of mecA was screened by PCR in all CoNS, and it showed that 36.3% isolates contained resistance gene mecA and were resistance to oxacillin, and mecA is a marker resistance gene of methicillin-resistant S. aureus （MRSA）.

Key words: poultry houses, bioaerosols, CoNS, drug-resistance, mecA

中图分类号:

S858.31

卢苑青, 张腾飞, 邵华斌, 曾驰. 鸡舍空气中葡萄球菌的分离鉴定及耐药性分析[J]. 湖北农业科学, 2020, 59(16): 118-121.

LU Yuan-qing, ZHANG Teng-fei, SHAO Hua-bin, ZENG Chi. Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(16): 118-121.

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鸡舍空气中葡萄球菌的分离鉴定及耐药性分析

Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses

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