四种培养基对大丽轮枝菌V991b及突变体的影响

doi:10.14088/j.cnki.issn0439-8114.2020.16.016

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (16): 78-84.doi: 10.14088/j.cnki.issn0439-8114.2020.16.016

四种培养基对大丽轮枝菌V991b及突变体的影响

黄薇¹, 袁斌¹, 金利容¹, 杨小林¹, 张瑞洋², 万鹏¹

1.湖北农业科学院植保土肥研究所/农业农村部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室,武汉 430064;
2.湖北工业大学工业设计学院,武汉 430068

收稿日期:2020-05-18 出版日期:2020-08-25 发布日期:2020-10-09
通讯作者: 万鹏（1972-）,男,湖北监利人,研究员,博士,主要从事农业昆虫与转基因环评研究,（电子信箱）wanpenghb@126.com。
作者简介:黄薇（1981-）,女,湖北武汉人,助理研究员,硕士,主要从事棉花病害防治研究,（电话）13487097672（电子信箱）34966440@qq.com
基金资助:
湖北省农业科技创新中心资助项目（2016-620-000-001-016）; 国家转基因新品种培育重大专项（2016ZX08012004-006）

The effect of four kinds of medium types on Verticillium dahliae V991b and its mutant

HUANG Wei¹, YUAN Bin¹, JIN Li-rong¹, YANG Xiao-lin¹, ZHANG Rui-yang², WAN Peng¹

1. Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management Crops in Central China, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Wuhan 430064,China;
2. School of Industrial Design,Hubei University of Technology, Wuhan 430068,China

Received:2020-05-18 Online:2020-08-25 Published:2020-10-09

摘要/Abstract

摘要： 为了解黄萎病菌V991b及其突变体V991w的生物学特性和致病力,调查了不同条件培养基对2种病原菌的生长及致病力的影响。结果表明,2种病原菌的生长曲线基本呈线性。V991b在PDA培养基上的生长速率与其他培养基之间差异显著,其他3种培养基之间差异不显著;V991w在PDA和PCA培养基上的生长速率之间差异不显著,与其他两种培养基之间差异显著。接种于PDA培养基15 d后,V991b与V991w的菌落生长直径之间无显著差异。经查氏液体培养基摇菌后接种棉苗,25 d时V991b和V991w的致病力分别为56.63和23.23 , 而经查氏-棉叶组织液体培养基摇菌后接种棉苗,其病情指数明显降低,接种25 d时V991b和V991w的病指仅为12.12和7.32。棉叶组织培养基对大丽轮枝菌微菌核的直径生长无显著影响,但对微菌核的数量和菌丝生长有明显的抑制作用。

关键词: 黄萎病, 培养性状, 生长速率, 菌落直径, 致病力

Abstract: In order to understand the biological characteristics and pathogenicity of Verticillium dahilae V991b and its mutant V991w, the effects of different conditioned medium on the growth and pathogenicity of the two pathogens were investigated. The results showed that the growth curve of two strains on different types of medium was linear basically. The growth rate of V991b on PDA was significantly different from other types of medium. And the growth rate of V991w on PDA and PCA medium was significantly different from other two types of medium. After cultiviated 15 days on PDA medium, there was no significantly difference between the colony diameter of V991b and that of V991w. During amplified the fungi in Czapek’s liquid medium and then inoculated the cotton seedling, the pathogenicity of V991b and V991w was quite different. The disease index on 25 days of inoculated was 56.63 and 23.23, respectively. However, the disease index of cotton seedlings inoculated with Czapek’s-cotton leaf tissue liquid medium was significantly reduced, and the disease index of V991b and V991w at 25 d inoculation was only 12.12 and 7.32. The plant tissue culture medium had no significant effect on the diameter of the microsclerotia, but it had significant inhibitory effect on the number of microsclerotia and the growth of hypha.

Key words: Verticillium dahliae, cultural characteristics, the growth rate, colony diameter, pathogenicity

中图分类号:

S432.4⁺4

黄薇, 袁斌, 金利容, 杨小林, 张瑞洋, 万鹏. 四种培养基对大丽轮枝菌V991b及突变体的影响[J]. 湖北农业科学, 2020, 59(16): 78-84.

HUANG Wei, YUAN Bin, JIN Li-rong, YANG Xiao-lin, ZHANG Rui-yang, WAN Peng. The effect of four kinds of medium types on Verticillium dahliae V991b and its mutant[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(16): 78-84.

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四种培养基对大丽轮枝菌V991b及突变体的影响

The effect of four kinds of medium types on Verticillium dahliae V991b and its mutant

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