CpG ODN纳米佐剂的免疫效应及应用进展

doi:10.14088/j.cnki.issn0439-8114.2020.13.002

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (13): 10-13.doi: 10.14088/j.cnki.issn0439-8114.2020.13.002

CpG ODN纳米佐剂的免疫效应及应用进展

王征帆¹, 万曾培¹, 高小鹏¹, 应碧云¹, 向蕊¹, 李祎云¹, 庞旋飞², 李中圣², 白挨泉¹

1.佛山科学技术学院,广东佛山 528231;
2.广东海大畜牧兽医研究院有限公司,广州 511400

收稿日期:2019-12-01 出版日期:2020-07-10 发布日期:2020-09-03
通讯作者: 李中圣( 1979-),男,山西大同人,高级兽医师,博士,主要从事免疫学及基因工程技术应用研究,(电子信箱)lizs@haid.com.cn;白挨泉(1970-),男,山西吕梁人,教授,硕士,主要从事猪传染病诊断与防治工作,(电子信箱)baiai-quan2008@163.com。
作者简介:王征帆( 1993-),男,贵州雷山人,在读硕士研究生,研究方向为猪传染病诊断与防治,(电话) 15320520429(电子信箱)15320520429@l63.com。
基金资助:
广东省教育厅预防兽医学重点实验室项目(2014KTSPT037); 广东省省级科技计划项目(2015B020203006); 广东省现代农业产业技术体系生猪创新团队项目( 2019KJ126)

Progress in immunological effects and application of CpG ODN nano adjuvant

WANG Zheng-fan¹, WAN Zeng-pei¹, GAO Xiao-peng¹, YING Bi-yun¹, XIANG Rui¹, LI Yi-yun¹, PANG Xuan-fei², LI Zhong-sheng², BAI Ai-quan¹

1. Foshan University, Foshan 528231,Guangdong, China;
2. Guangdong Haida Animal Husbandry and Veterinary Research Institute Co. LTD, Guangzhou 511400,China

Received:2019-12-01 Online:2020-07-10 Published:2020-09-03

摘要/Abstract

摘要： CpG ODN是人工合成的具有非特异性免疫刺激的DNA序列,模拟细菌DNA在体内的应答过程,诱发细胞和体液免疫。CpG ODN作为新型免疫增强剂在兽医临床上的应用展现出了巨大的潜力,并在免疫学、药物学和药效学等领域备受关注。然而CpG ODN并不稳定且易被核酸酶酶解,其自身带负电荷,不易结合到细胞表面。因此,CpG ODN如何高效传递并能有效摄取成了新的挑战。纳米传递系统的研究使CpG ODN单独或协同疫苗免疫取得显著疗效,为改善CpG ODN在疫苗研究中的应用拓宽了思路。从CpG ODN纳米佐剂的作用机制、免疫活性、安全性及临床应用等方面进行综述,并对该领域未来的研究方向进行展望,为后续工作提供有益参考。

关键词: CpG ODN, 纳米佐剂, 作用机制, 免疫活性, 安全性

Abstract: CpG ODN is a synthetic DNA sequence with non-specific immunostimulation which simulates the response process of bacterial DNA in vivo, inducing cellular and humoral immunity. CpG ODN has shown great potential as a novel immunopotentiator in veterinary clinical applications, and has attracted much attention in the fields of immunology, pharmacology and pharmacodynamics. However, CpG ODN is unstable and easily digested by nucleases, which are negatively charged and do not easily bind to the cell surface. Therefore, how to efficiently transfer CpG ODN and effectively ingest it becomes a new challenge. The study of nano-transmission system has achieved significant efficacy for CpG ODN alone or in combination with vaccine immunization, and broadened the thinking for improving the application of CpG ODN in vaccine research. The mechanism of action, immunological activity, safety and clinical application of CpG ODN nanoadjuvant were summarized, and future research directions in this field was prospected, to provide useful reference for follow-up work.

Key words: CpG ODN, nano adjuvant, mechanism of action, immunological activity, safety

中图分类号:

Q291

王征帆, 万曾培, 高小鹏, 应碧云, 向蕊, 李祎云, 庞旋飞, 李中圣, 白挨泉. CpG ODN纳米佐剂的免疫效应及应用进展[J]. 湖北农业科学, 2020, 59(13): 10-13.

WANG Zheng-fan, WAN Zeng-pei, GAO Xiao-peng, YING Bi-yun, XIANG Rui, LI Yi-yun, PANG Xuan-fei, LI Zhong-sheng, BAI Ai-quan. Progress in immunological effects and application of CpG ODN nano adjuvant[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(13): 10-13.

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CpG ODN纳米佐剂的免疫效应及应用进展

Progress in immunological effects and application of CpG ODN nano adjuvant

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