基于ICP-MS的拟穴青蟹蜕壳期前后蟹肉Ca2+、Mg2+浓度变化研究

doi:10.14088/j.cnki.issn0439-8114.2020.12.025

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (12): 114-116.doi: 10.14088/j.cnki.issn0439-8114.2020.12.025

基于ICP-MS的拟穴青蟹蜕壳期前后蟹肉Ca²⁺、Mg²⁺浓度变化研究

周冬仁, 林锋, 孙博弈, 盛鹏程, 高晟, 郝贵杰, 叶雪平

农业农村部淡水渔业健康养殖重点实验室/浙江省鱼类健康与营养重点实验室/浙江省淡水水产研究所,浙江湖州 313001

收稿日期:2019-06-10 发布日期:2020-08-26
通讯作者: 叶雪平,推广研究员,（电子信箱）yxp 900@sina.com。
作者简介:周冬仁（1980-）,男,江苏江阴人,高级工程师,主要从事渔业环境与安全,（电话）13567287353（电子信箱）872559718@qq.com
基金资助:
浙江省科技厅重点研发项目（2018C02019）

Study on Ca²⁺, Mg²⁺ concentration in crab meat before and after molting stage of Scylla paramamosain by ICP-MS

ZHOU Dong-ren, LIN Feng, SUN Bo-yi, SHENG Peng-cheng, GAO Sheng, HAO Gui-jie, YE Xue-ping

Ministry of Agriculture and Rural Affairs Key Laboratory of Healthy Freshwater Aquaculture/ Key Laboratory of Fish Health and Nutrition of Zhejiang Province/Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, Zhejiang, China

Received:2019-06-10 Published:2020-08-26

摘要/Abstract

摘要： 采用石墨微波消解技术处理拟穴青蟹（Scylla paramamosain）蟹肉样品,ICP-MS 同时测定Ca、Mg两种元素,标准曲线相关系数均≥0.995,前处理耗时短,消解效果良好。结果表明,拟穴青蟹在硬壳期时体内Ca²⁺、Mg²⁺平均含量为471、2 508 mg/kg,而蜕壳后分别为385、1 565 mg/kg,拟穴青蟹蜕壳前后Mg²⁺浓度差异不显著、Ca²⁺浓度差异显著。因此,为使拟穴青蟹生长过程中甲壳顺利蜕壳及硬化,建议在养殖池中合理补充Ca²⁺、Mg²⁺。

关键词: 拟穴青蟹（Scylla paramamosain）, Ca²⁺, Mg²⁺, 电感耦合等离子体-质谱

Abstract: The meat samples of Scylla paramamosain were treated by graphite microwave digestion. ICP-MS was used to determine Ca²⁺ and Mg²⁺ simultaneously. The correlation coefficient of standard curve was ≥ 0.995. The pretreatment time was short and the digestion effect was good. The results showed that the average contents of Ca²⁺ and Mg²⁺ in S. paramamosain were 471 and 2 508 mg/kg in hard shell stage, 385 and 1 565 mg/kg in molting stage respectively. there had no significant differences in Mg²⁺, and had significant difference in Ca²⁺ concentration before and after molting stage. In order to make the Scylla molt and harden smoothly during the growth of the crab, it was suggested that Ca²⁺, Mg²⁺ should be supplemented reasonably in the culture pond.

Key words: Scylla paramamosain, Ca²⁺, Mg²⁺, ICP-MS

中图分类号:

S968.25⁺1

周冬仁, 林锋, 孙博弈, 盛鹏程, 高晟, 郝贵杰, 叶雪平. 基于ICP-MS的拟穴青蟹蜕壳期前后蟹肉Ca²⁺、Mg²⁺浓度变化研究[J]. 湖北农业科学, 2020, 59(12): 114-116.

ZHOU Dong-ren, LIN Feng, SUN Bo-yi, SHENG Peng-cheng, GAO Sheng, HAO Gui-jie, YE Xue-ping. Study on Ca²⁺, Mg²⁺ concentration in crab meat before and after molting stage of Scylla paramamosain by ICP-MS[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(12): 114-116.

参考文献

[1] HARTNOLL R G.The Biology of Crustacea. Embryology,Morphology and Genetics[M]. New York:Academic press,1982.111-185.
[2] 朱春华,徐志标. 淡水养殖水体Ca²+与Mg²+含量对南美白对虾生长的影响[J]. 淡水渔业,2002,32(6):46-48.
[3] 曾朝曙,王桂忠,李少菁. 锯缘青蟹胚胎发育的观察及温度影响胚胎发育的研究[J]. 福建水产,1991 (1):45-50.
[4] 王洪全,黎志福. 水温、盐度双因子交互作用对河蟹胚胎发育的影响[J]. 湖南师范大学自然科学学报,1996,19(3):63-66.
[5] 赵云龙,孟凡丽,陈立侨,等. 不同水温对红螯螯虾胚胎发育的影响[J]. 湖泊科学,2000,12(1): 59-62.
[6] 岳立芳,廖永岩,刘家良,等. 海水钙浓度对远海梭子蟹软壳持续时间与形态变化的影响[J]. 广东海洋大学学报,2010,31(3):90-95.
[7] 谢达祥,陈晓汉,黄均,等. Ca²+与Mg²+浓度对凡纳滨对虾糠虾幼体育成仔虾成活率和生长的影响[J]. 西南农业学报,2007,20(4):787-794.
[8] 黄根东. 补钙在水产养殖中的应用[J]. 科学养鱼,2013(12):87.
[9] SABRIYE S,FATIH A E,FATMA T,et al.Simultaneous determination of chromium species in water and plant samples at trace levels by ion chromatography-Inductively coupled plasma-mass Spectrometry[J]. Analytical letters,2018,52(5):761-771.
[10] 黄春红,杨品红,王文彬. ICP-AES法对珍珠蚌养殖环境中矿物质的测定[J]. 湖北农业科学,2013,52(17),4210-4213.
[11] 黄波,程开敏,刘艳妮,等. 对虾矿物元素营养需求的研究进展[J]. 饲料博览,2006(10):12-15.
[12] 侯文杰,臧维玲,刘永士, 等. 盐度及Ca²+与Mg²+含量对凡纳滨对虾生长及虾体钙镁含量的影响[J]. 江苏农业科学,2012,40(3):193-196.
[13] 王武,王成辉,马旭洲. 河蟹生态养殖[M]. 第2 版. 北京:中国农业出版社,2013. 201-239.
[14] 宋博,曹善茂,孙凯. 黄金米虾养殖用水中钙镁离子的含量与比例[J]. 水产科学,2015,34(6):364-369.

基于ICP-MS的拟穴青蟹蜕壳期前后蟹肉Ca²⁺、Mg²⁺浓度变化研究

Study on Ca²⁺, Mg²⁺ concentration in crab meat before and after molting stage of Scylla paramamosain by ICP-MS

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