湖北农业科学 ›› 2020, Vol. 59 ›› Issue (11): 47-50.doi: 10.14088/j.cnki.issn0439-8114.2020.11.010

• 资源·环境 • 上一篇    下一篇

壶瓶碎米荠对硒酸钠胁迫响应研究

罗金玲a, 杨珖a, 陈鸿辉b, 曾维超a, 王立a, 陈大清b   

  1. 仲恺农业工程学院, a. 园艺园林学院; b. 农业与生物学院, 广州 510225
  • 收稿日期:2019-03-27 出版日期:2020-06-10 发布日期:2020-08-06
  • 通讯作者: 陈大清, 男, 教授, (电话)13560380388(电子信箱)daqingchen@vip.sina.com。
  • 作者简介:罗金玲(1990-), 女, 广东阳春人, 在读硕士研究生, 研究方向为园艺农产品功能营养开发, (电话)15112163182(电子信箱)1759002868@qq.com;
  • 基金资助:
    国家自然科学基金项目(31560579); 广东省科技计划项目(2015A030302081); 广东省教育厅人才引进项目(粤财教2010343号); 广东省技术质量监督局项目(粤财农[2017]66号)

Studies on physiological effect of sodium selenate stress in Cardamine hupingshanensis

LUO Jin-linga, YANG Guanga, CHEN Hong-huib, ZENG Wei-chaoa, WANG Lia, CHEN Da-qingb   

  1. a.College of Horticulture and Landscape Architecture; b.College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
  • Received:2019-03-27 Online:2020-06-10 Published:2020-08-06

摘要: 通过在MS培养基中添加不同浓度的硒酸钠溶液(0、20、40、60、80、100、200、300 mg/L)来进行壶瓶碎米荠(Cardamine hupingshanensis)种子萌发培养, 测定种子萌发率、幼苗成活率、黄化率、植株高度、根长、鲜重和干重、POD活性及CAT活性等生理生化指标, 以探讨壶瓶碎米荠对硒酸钠胁迫的响应。结果表明, (1)低浓度的硒酸钠溶液促进壶瓶碎米荠植株生长量和生物量的增加, 反之则抑制;(2)在40 mg/L硒酸钠溶液处理下, 壶瓶碎米荠的发芽率和成苗率最高, 且幼苗的根长、株高、鲜重和干重均达到最大值;(3)壶瓶碎米荠幼苗的黄化率随着硒酸钠溶液浓度的升高而升高;(4)壶瓶碎米荠的POD活性呈先降低后升高的趋势, 在40 mg/L硒酸钠溶液处理时达到最低值;(5)壶瓶碎米荠的CAT活性总体上呈先升高后降低的趋势, 在100 mg/L硒酸钠溶液处理时达到最高值。

关键词: 壶瓶碎米荠(Cardamine hupingshanensis), 硒胁迫, 生理特性

Abstract: Cardamine hupingshanensis seeds was cultivated in MS culture medium with different sodium selenate concentration (0, 20, 40, 60, 80, 100, 200, 300 mg/L). The physiological and biochemical indexes such as seed germination rate, seedling survival rate, yellowing rate, plant height, root length, fresh weight, dry weight, POD activity and CAT activity were measured to discuss the response of selenate stress in pots to Cardamine hupingshanensis. The results showed that, the low concentration of sodium selenate was used to promote the growth and biomass of the Cardamine hupingshanensis, on the contrary, it would inhibit it. When the concentration of sodium selenate was 40 mg/L, Cardamine hupingshanensis had the highest germination rate and seedling rate, at the same time, the root length, plant height, fresh weight and dry weight of the seedlings reached the maximum value. The yellowing rate of Cardamine hupingshanensis seedlings increased with the increase of selenium salt concentration. The POD activity of the Cardamine hupingshanensis was decreased first and then increased, the lowest value reached at 40mg/L sodium selenate solution. The CAT activity of the Cardamine hupingshanensis was increased first and then decreased, the highest value reached at 100 mg/L sodium selenate solution.

Key words: Cardamine hupingshanensis, Se stress, physiological property

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