低温胁迫下贵州云雾贡茶生长调节剂的变化

doi:10.14088/j.cnki.issn0439-8114.2020.08.022

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (8): 99-102.doi: 10.14088/j.cnki.issn0439-8114.2020.08.022

低温胁迫下贵州云雾贡茶生长调节剂的变化

王莹^1,2,3, 李岩^1,3, 王姝⁴, 向准², 赵德刚^1,3

1.贵州大学生命科学学院/农业生物工程研究院/贵州省农业生物工程重点实验室/山地植物资源保护与种质创新省部共建教育部重点实验室,贵阳 550025;
2.贵州省生物研究所,贵阳 550009;
3.贵州省山地生态与农业生物工程2011协同创新中心,贵阳 550025;
4.黑龙江职业学院,哈尔滨 150111

收稿日期:2019-08-20 出版日期:2020-04-25 发布日期:2020-07-03
通讯作者: 赵德刚（1961-）,男,教授,博士生导师,主要从事植物基因工程研究,（电子信箱）dgzhao@gzu.edu.cn。
作者简介:王莹（1982-）,女,黑龙江齐齐哈尔人,副研究员,在读博士研究生,主要从事植物分子生物学遗传育种方面的研究,（电话）18111862560（电子信箱）wangyinghouzi@163.com。
基金资助:
贵州省高层次创新型人才培养“千层次”计划项目; 山地植物资源保护与种质创新省部共建教育部重点实验室开放基金资助项目（MOELP-201703）; 贵州省科技基金项目[黔科合基础（2018）1044]

Changes of growth regulators of Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen under low temperature stress in Guizhou

WANG Ying^1,2,3, LI Yan^1,3, WANG Shu⁴, XIANG Zhun², ZHAO De-gang^1,3

1. The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region （Ministry of Education）/Guizhou Key Lab of Agro-Bioengineering,Institute of Agro-Bioengineering, College of Life Sciences,Guizhou University,Guiyang 550025,China;
2. Guizhou Province Institute of Biology,Guiyang 550009,China;
3. The 2011 Collaboration Innovation Center for Mountain Ecology and Agro-Bioengineering,Guiyang 550025,China;
4. Heilongjiang Institute of Technology,Herbin 150111,China

Received:2019-08-20 Online:2020-04-25 Published:2020-07-03

摘要/Abstract

摘要： 以贵州云雾贡茶[Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen]一年生幼苗茶树为试验材料,用酶联免疫法（ELISA）测定不同低温胁迫下茶树叶片生长调节剂含量的变化,以探讨低温胁迫下茶树生长调节剂变化规律。结果显示,1、4 ℃处理云雾贡茶IAA、GA含量大部分显著低于对照,ABA含量、ABA/GA明显高于对照;10 ℃处理ABA、IAA、GA含量大部分显著高于对照,ABA/GA与对照无明显变化。随胁迫时间延长,1、4 ℃处理ABA、IAA含量随时间变化呈先上升后下降的趋势,GA含量呈下降趋势,ABA/GA呈上升趋势;10 ℃处理ABA和GA含量呈先上升后下降的趋势,IAA含量呈下降趋势。低温条件下,云雾贡茶能提高ABA类生长抑制物质,降低IAA、GA等生长促进类物质,通过调节植物体内生长调节剂平衡来应对低温胁迫。

关键词: 云雾贡茶[Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen], 低温胁迫, 生长调节剂

Abstract: To understand the changes of growth regulators in tea plant [Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen] under the different low-temperature in Guizhou province, one-year-old tea plants of the cultivar were used as the material and the changes of growth regulators content were determined by enzyme-linked immunosorbent assay （ELISA）. Compared to the control, Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen mostly exhibited lower contents in IAA、GA, and ABA content,ABA/GA were higher than control under the 4 ℃and 1 ℃ treatment. While mostly contents of ABA、IAA、GA were significantly higher than control and ABA/GA was no significant change in the 10 ℃ treatment. With the increase of stress time, the content of ABA and IAA increased first and then decreased with the change of time, the content of GA decreased, the ratio of ABA/GA increased, and the content of ABA and GA increased first and then decreased at 10 ℃, the content of IAA decreased. Under low temperature, Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen can improve the growth inhibiting substance such as ABA and reduce the growth promoting substances such as IAA and GA, in order to adjust the endogenous hormonesbalance in plants to cope with low temperatures.

Key words: Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen, low temperature stress, growth regulator

中图分类号:

王莹, 李岩, 王姝, 向准, 赵德刚. 低温胁迫下贵州云雾贡茶生长调节剂的变化[J]. 湖北农业科学, 2020, 59(8): 99-102.

WANG Ying, LI Yan, WANG Shu, XIANG Zhun, ZHAO De-gang. Changes of growth regulators of Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen under low temperature stress in Guizhou[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 99-102.

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低温胁迫下贵州云雾贡茶生长调节剂的变化

Changes of growth regulators of Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen under low temperature stress in Guizhou

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