湖北农业科学 ›› 2020, Vol. 59 ›› Issue (8): 80-82.doi: 10.14088/j.cnki.issn0439-8114.2020.08.018

• 植物保护 • 上一篇    下一篇

几种抗菌素对豌豆根边缘细胞培养液的抑菌测定

王芳, 乔美星, 喻敏   

  1. 佛山科学技术学院,广东 佛山 528000
  • 收稿日期:2019-12-18 出版日期:2020-04-25 发布日期:2020-07-03
  • 作者简介:王 芳(1964-),女,山东莱阳人,副教授,硕士,主要从事植物真菌病害研究,(电话)18689286491(电子信箱)1940480985@qq.com。

Determination of bacterial inhibitory fluid of fringe cells in pea root by several antibiotics

WANG Fang, QIAO Mei-xing, YU Min   

  1. Foshan University,Foshan 528000,Guangdong,China
  • Received:2019-12-18 Online:2020-04-25 Published:2020-07-03

摘要: 采用液体培养的方法,测定了5种抗菌素对豌豆根边缘细胞培养液污染细菌的抑制和对边缘细胞的降解作用。结果表明,5种抗菌素在50~4 000 mg/L的浓度梯度内,对边缘细胞培养液污染细菌均具有一定的抑制作用。其中,罗红霉素、头孢呋辛酯和恩诺沙星对污染细菌抑制率较适宜的浓度分别为2 000、 1 000和1 000 mg/L,抑制率分别达93.48%、58.70%和91.30%。青霉素、罗红霉素、头孢呋辛酯、庆大霉素和恩诺沙星分别在500、500、1 000、1 000和500 mg/L时对边缘细胞的降解作用较缓慢,分别为63.89%、58.95%、60.80%、73.15%和58.95%,浓度增高,对边缘细胞的影响会明显增强。

关键词: 抗菌素, 污染细菌, 边缘细胞, 抑制作用

Abstract: By means of liquid culture, the inhibition of 5 antibiotics on bacteria contaminated by culture medium and degradation of edge cells of pea root were determined. The results showed that the five kinds of antibiotics in 50~4 000 mg/L concentration gradient, in both the edge bacterial cell cultures of certain inhibitory effect. Among them, the appropriate selective concentrations of roxithromycin, cefuroxime octyl and enol were 2 000, 1 000 and 1 000 mg/L, respectively, and the inhibitory rates reached 93.48%, 58.70% and 91.30%, respectively. The degradation of penicillin, roxithromycin, cefuroxime octyl, gentamicin and enol at 500, 500, 1 000, 1 000 and 500 mg/L on marginal cells was slow, which was 63.89%, 58.95%, 60.80%, 73.15% and 58.95%, respectively. The effect on marginal cells was significantly enhanced with the increase of concentration.

Key words: antibiotics, bacterial contamination, marginal cells, inhibition

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