抗菌植物培养基的制备及抗菌性能研究

doi:10.14088/j.cnki.issn0439-8114.2020.03.015

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (3): 79-81.doi: 10.14088/j.cnki.issn0439-8114.2020.03.015

抗菌植物培养基的制备及抗菌性能研究

张芯^1,2,3, 陈婧雯¹

1.安康学院化学化工学院/新型材料研究中心,陕西安康 725000;
2.安康市铁/铝基纳米新材料工程技术研究中心,陕西安康 725000;
3.安康市锌基纳米材料工程技术研究中心,陕西安康 725000

收稿日期:2019-05-15 发布日期:2020-05-11
作者简介:张芯（1987-）,女,陕西安康人,讲师,硕士,主要从事纳米材料制备及应用研究,（电话）18291529057（电子信箱） zhangxin0621@sina.com。
基金资助:
安康学院科技扶贫专项（2018FPZX01）; 陕西省科技厅平台子项目（2018PT-31）; 安康市重大科研攻关项目（2016AKZDCY002）

Preparation of antimicrobial plant medium and research of its antibacterial properties

ZHANG Xin^1,2,3, CHEN Jing-wen¹

1.Department of Chemistry and Chemical Engineering/Research Centre of New Materials,Ankang University,Ankang 725000,Shaanxi,China;
2.Engineering Research Center of Iron/Aluminum Based Nano-materials,Ankang 725000,Shaanxi,China;
3.Engineering Research Center of Zinc-based Nanomaterials,Ankang 725000,Shaanxi,China

Received:2019-05-15 Published:2020-05-11

摘要/Abstract

摘要： 通过液相还原硝酸银得到纳米银粒子,然后将其添加入植物组织培养基中,从而得到一种抗菌的复合培养基,实现植物组织的开放式培养。试验主要包括纳米银粒子的制备及复合培养基的制备。首先,采用一种绿色无毒的方法,利用果糖和葡萄糖作为还原剂还原AgNO₃溶液,同时研究硝酸银浓度对纳米银粒子粒径的影响。在此基础上,制备出纳米银粒子复合的B5培养基。结果表明,随着AgNO₃溶液浓度的升高,纳米银粒子的尺寸增大,粒径分布范围变宽;抗菌测试结果表明,纳米银粒子复合的B5培养基具有显著的抗菌性能。

关键词: 植物培养基, 纳米银, 抗菌性能

Abstract: Nanosilver particles were obtained by liquid phase reduction of AgNO₃, and nanosilver composite plant medium with good antimicrobial capability was successfully prepared to realize open culture of plant tissues. The experiment mainly included the preparation of nanosilver particles and the preparation of composite plant medium. First, nanosilver particles were prepared in AgNO₃ solution using fructose and D-glucose as reduction reagents. The effect of AgNO₃ concentration on the nanoparticle size was investigated. Second, the solvent containing the nanosilver particles was added in B5 medium to form a composite plant medium. It was found that with the increase of AgNO₃ solution concentration, the average size of nanosilver particles increased, and the size distribution range became wider. Moreover, the composite B5 medium containing the nanosilver particles exhibited significant antimicrobial capability.

Key words: plant medium, nanosilver, antimicrobial capability

中图分类号:

Q-331

张芯, 陈婧雯. 抗菌植物培养基的制备及抗菌性能研究[J]. 湖北农业科学, 2020, 59(3): 79-81.

ZHANG Xin, CHEN Jing-wen. Preparation of antimicrobial plant medium and research of its antibacterial properties[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(3): 79-81.

抗菌植物培养基的制备及抗菌性能研究

Preparation of antimicrobial plant medium and research of its antibacterial properties

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