放线菌JK-1的鉴定及其对稻瘟病菌的抑菌活性

doi:10.14088/j.cnki.issn0439-8114.2018.24.027

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (24): 96-99.doi: 10.14088/j.cnki.issn0439-8114.2018.24.027

放线菌JK-1的鉴定及其对稻瘟病菌的抑菌活性

曾凡松¹, 李素芹², 王运凤², 刘启梅², 向礼波¹, 杨立军¹, 龚双军¹, 史文琦¹

1.农业农村部华中作物有害生物综合治理重点实验室/湖北省农业科学院植保土肥研究所,武汉 430064;
2.宜都市农业局,湖北宜都 443300

收稿日期:2018-09-30 出版日期:2018-12-25 发布日期:2020-04-01
作者简介:曾凡松（1980-）,男,湖北宜昌人,副研究员,博士,主要从事水稻和小麦病害防治研究,（电话）13476087392（电子信箱）zengfansong2005@126.com。
基金资助:
湖北省农业科技创新中心资助项目（2016-620-000-001-015）

Identification of An Actinomyce Isolate JK-1 and Its Antifungal Activity Against the Rice Blast Fungus

ZENG Fan-song¹, LI Su-qin², WANG Yun-feng², LIU Qi-mei², XIANG Li-bo¹, YANG Li-jun¹, GONG Shuang-jun¹, SHI Wen-qi¹

1.Laboratory of Integrated Pest Management on Crop in Central China,Ministry of Agriculture and Rural Affairs/Institute for Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences,Wuhan 430064,China;
2.Agricultural Bureau of Yidu County, Yidu 443300,Hubei,China

Received:2018-09-30 Online:2018-12-25 Published:2020-04-01

摘要/Abstract

摘要： 对放线菌菌株JK-1的形态学、生理生化特征和16S rRNA基因序列进行了分析。结果表明,菌株JK-1为微白黄链霉菌变种（Streptomyces albidoflavus var.）。为明确JK-1对4种植物病原真菌,稻瘟病菌（Magnaporthe oryzae）、棉花黄萎病菌（Verticillium alboatrum）、小麦赤霉病菌（Fusarium graminearum）和水稻纹枯病菌（Rhizoctonia solani Kühn）的抑制活性,采用菌丝生长速率法进行了抑菌活性测定。该菌株粗发酵液对4种真菌的菌丝生长均有显著抑制作用,尤其是对稻瘟病菌,抑制率达到91.14%±0.04%。进一步对粗发酵液进行了冻干浓缩和甲醇提取。提取液在稀释2 000倍后对稻瘟病菌菌丝生长抑制率为53.42%±1.29%,表现出优良的抑菌活性。

关键词: 链霉菌（Streptomyces）, 分类鉴定, 稻瘟病菌（Magnaporthe orgzae）, 抑菌活性

Abstract: Morphological,physiological and biochemical characteristics and 16S rRNA gene sequence of actinomyce isolate JK-1 were analysed. It showed that isolate JK-1 belonged to Streptomyces albidoflavus var. . To evaluate the inhibiting activities of isolate JK-1 against four plant fungal pathogens,the rice blast fungus（Magnaporthe oryzae）,the cotton verticillium wilt pathogen （Verticillium alboatrum）,the fusarium head blight pathogen （Fusarium graminearum） and rice sheath blight pathogen （Rhizoctonia solani Kühn）,antifungal activity assays were performed using mycelium growth rate method. The crude fermentation liquid showed strong inhibiting activity against the four pathogens,especially M. oryzae with inhibitory rate of 91.14%±0.04%. The crude fermentation liquid was concentrated by freeze drying,and then extracted with methanol. The diluted methanol extraction of fermentation liquid with 2 000 times exhibited a significant inhibitory effect on mycelium growth of M. oryzae with inhibiting rate of 53.42%±1.29%.

Key words: Streptomyces, classification and identification, the rice blast fungus （Magnaporthe oryzae）, antimicrobial activity

中图分类号:

S435.111.4⁺1

曾凡松, 李素芹, 王运凤, 刘启梅, 向礼波, 杨立军, 龚双军, 史文琦. 放线菌JK-1的鉴定及其对稻瘟病菌的抑菌活性[J]. 湖北农业科学, 2018, 57(24): 96-99.

ZENG Fan-song, LI Su-qin, WANG Yun-feng, LIU Qi-mei, XIANG Li-bo, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi. Identification of An Actinomyce Isolate JK-1 and Its Antifungal Activity Against the Rice Blast Fungus[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(24): 96-99.

参考文献

[1] SKAMNIOTI P,GURR S J.Against the grain:Safeguarding rice from rice blast disease[J].Trends Biotechnol,2009,27(3):141-150.
[2] POOJA K,KATOCH A.Past,present and future of rice blast management[J].Plant Sci Today,2014,1(3):165-173.
[3] 吴志华,李苹,柏连阳,等.湖南省水稻稻瘟病菌对稻瘟灵抗药性研究[J].安徽农业科学,2011,39(28):17279-17280.
[4] 姚树萍,贾丽,何玲,等.我国水稿稻瘟病菌对三环唑抗性研究现状[J].农药科学与管理,2017,38(6):13-17.
[5] LAW J W,SER H L,KHAN T M,et al.The potential of Streptomycesas biocontrol agents against the rice blast fungus, Magnaporthe oryzae(Pyricularia oryzae)[J].Front Microbiol,2017, 8(3):1-10.
[6] 张炳火,李汉全,徐阳,等.链霉菌JXJ402抗稻瘟病菌的活性成分[J].江苏农业学报,2009,25(2):282-285.
[7] 廖红东,袁珊珊,杨远柱,等.一株拮抗稻瘟病内生链霉菌OsiSh-10的筛选与鉴定[J].湖南大学学报(自然科学版),2015,42(12):80-87.
[8] 邵正英,陈业欣,熊超,等.链霉菌JD211对接种稻瘟病后水稻过敏性反应的生理机制分析[J].西北植物学报,2017,37(7):1340-1347.
[9] 王真真,徐婷,袁珊珊,等.水稻内生放线菌OsiRt-1的分离鉴定及对稻瘟病的防治作用[J].微生物学通报,2016,43(5):1009-1018.
[10] XU B,CHEN W,WU Z M,et al.A novel and effective Streptomyces sp. N2 against various phytopathogenic fungi[J].Appl Biochem Biotech,2015,177(6):1338-1347.
[11] PRABAVATHY V R,MATHIVANAN N,MURUGESAN K.Control of blast and sheath blight diseases of rice using antifungal metabolites produced by Streptomyces sp. PM5[J].Biol Control,2006,39(3):313-319.
[12] 张学江,曾凡松,杨立军,等.抗稻瘟病链霉菌JK-1基因组BAC文库构建[J].安徽农学通报,2014,20(24):17-18,129.
[13] 中国科学院微生物研究所放线菌分类组.链霉菌鉴定手册[M].北京:科学出版社,1975.
[14] 阎逊初. 放线菌的分类和鉴定[M].北京:科学出版社,1992.
[15] WILLIAMS S T,DAVIES F L.Use of a scanning electron microscope for the examination of actinomycetes[J].J Gen Microbiol,1967,48(2):171-177.
[16] 张志斌,敖武,熊瑶瑶,等.内生拮抗放线菌FRo2的鉴定及抑菌活性物质的分离[J].微生物学通报,2014,41(8):1574-1581.
[17] NIGTHOUJAM D S,SANASAM S,TAMREIHAO K,et al.Antagonistic activities of local actinomycete isolates against rice fungal pathogens[J].Afr J Microbiol Res,2009,3(11):737-742.
[18] BOUKAEW S,PRASERTSAN P.Suppression of rice sheath blight disease using a heat stable culture filtrate from Streptomyces philanthi RM-1-138[J].Crop Prot,2014,61(3):1-10.
[19] KHALIL M S,MOUBASHER H,HASAN F F.Biological control of rice blast disease by Streptomyces flavotricini[J].Res J Pharm Biol Chem Sci,2014,5(5):1453-1461.
[20] QIN S,LI J,CHEN H H,et al.Isolation,diversity,and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna,China[J].Appl Environ Microbiol,2009,75(19):6176-6186.
[21] 周美英,郑志成.海洋放线菌S-216菌株鉴定及其抗真菌抗生素合成条件研究[J].厦门大学学报(自然科学版),1998,37(1):109-114.
[22] 张永娜,刘佳琳,徐德阳,等.2株微白黄链霉菌发酵液的稳定性及抑菌效果分析[J].中国农学通报,2017,33(12):108-114.
[23] 于素亚,潘华奇,胡江春,等.深海放线菌08A4的鉴定及其抗真菌活性产物研究[J].微生物学杂志,2012,32(5):1-5.

放线菌JK-1的鉴定及其对稻瘟病菌的抑菌活性

Identification of An Actinomyce Isolate JK-1 and Its Antifungal Activity Against the Rice Blast Fungus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	安福涛, 谢瑾卉, 林英, 于舒怡, 臧超群, 梁春浩. 苍白杆菌SY286抑菌活性蛋白稳定性分析[J]. 湖北农业科学, 2021, 60(8): 94-97.
[2]	纪若薇, 杨扬, 林鹏程. 头花杜鹃叶中精油成分及其抑菌活性研究[J]. 湖北农业科学, 2021, 60(3): 120-124.
[3]	熊永通, 胡津瑜, 王云萍, 吴清来, 李俊凯. N-烷基化甘氨酸乙酯-吩嗪-1-羧酸衍生物的合成及其抑菌活性[J]. 湖北农业科学, 2020, 59(16): 85-87.
[4]	刘长风, 张功领, 高品一, 刘学贵. 杂木木醋液成分分析及精制后再处理抑菌活性研究[J]. 湖北农业科学, 2019, 58(14): 76-79.
[5]	李朋钰, 李映, 杨涛, 田平芳, 田国英, 葛喜珍. 小檗碱与多种成分复配及其对番茄灰霉病的抑制作用[J]. 湖北农业科学, 2019, 58(1): 56-60.
[6]	黄建东, 钱勇, 杨士杰, 王鹏钧, 冯宝军, 谢天培. 4-氟苄酰胺类化合物的合成及其对白菜软腐病病菌的抑菌活性研究[J]. 湖北农业科学, 2018, 57(23): 94-97.
[7]	张垚, 白金峰, 徐凯莉, 刘宁. 具有抗菌活性的水杉内生真菌的分离及筛选[J]. 湖北农业科学, 2018, 57(11): 36-38.
[8]	郭佩佩, 张雪娟, 马永珍, 徐艳. 大孔树脂分离对银杏外种皮多酚抗氧化及抑菌活性的影响[J]. 湖北农业科学, 2018, 57(11): 75-78.