湖北农业科学 ›› 2018, Vol. 57 ›› Issue (12): 65-68.doi: 10.14088/j.cnki.issn0439-8114.2018.12.018

• 植物保护 • 上一篇    下一篇

辽宁树莓黏菌病病原菌原质团生物学特性研究

臧超群, 林秋君, 傅俊范2, 梁春浩   

  1. 1.辽宁省农业科学院,a.植物保护研究所;b.开放实验室,沈阳 110161;
    2.沈阳农业大学植物保护学院,沈阳 110866
  • 收稿日期:2018-02-23 发布日期:2020-03-31
  • 通讯作者: 梁春浩(1979-),男,研究员,主要从事植物病害生物防治研究,(电子信箱)liangchunhao99@126.com。
  • 作者简介:臧超群(1985-),男,安徽砀山人,助理研究员,博士,主要从事植物病害生物防治研究,(电话)15940340906(电子信箱)zangchaoqun111@126.com
  • 基金资助:
    辽宁省博士启动基金项目(20170520173)

Study on Biological Characteristics of Pathogen Protoplasts of Raspberry Mycetozoan Disease in Liaoning Province

ZANG Chao-qun, LIN Qiu-jun, FU Jun-fan2, LIANG Chun-hao   

  1. 1a.Institute of Plant Protection; 1b.Opening Laboratory,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China;
    2.College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China
  • Received:2018-02-23 Published:2020-03-31

摘要: 采用植物病理学研究方法,对树莓(Rubus idaeus L.)黏菌病病原菌黏菌原质团进行生物学特性测定。结果表明,黏菌原质团在马铃薯葡萄糖琼脂培养基、马铃薯蔗糖琼脂培养基、燕麦培养基、树莓叶粉培养基、査氏培养基、水琼脂培养基、孟加拉红培养基、玉米粉培养基8种培养基上均可生长,其中树莓叶粉培养基上生长最佳,菌落直径可达8.45 cm;最适碳源为乳糖和淀粉,最适氮源为精氨酸,最适pH为6,最适生长温度为30 ℃,最适培养条件为24 h连续黑暗。

关键词: 树莓(Rubus idaeus L.), 黏菌, 原质团, 生物学特性

Abstract: Using the phytopathological methods,the biological characteristics of mycetozoan isolated from raspberry(Rubus idaeus L.) was tested. The results showed that the plasmodium of mycetozoan could grow on the eight different mediums,such as PDA,PSA,oatmeal agar medium,raspberry leaf powder medium,czapek's medium,water agar medium,rose bengal medium,corn flour medium. Among these mediums the colony on raspberry leaf powder growed best, with the diameter up to 8.45 cm.Optimum carbon source was lactose and starch,and the optimum nitrogen source was arginine, the optimum pH was 6,the optimum growth temperature was 30 ℃,24 h continuous darkness had the best effect on colony growth.

Key words: raspberry(Rubus idaeus L.), mycetozoan, plasmodium, biological characteristics

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