放线菌产杀虫物质的初步鉴别及其活性评价

doi:10.14088/j.cnki.issn0439-8114.2018.07.014

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (7): 61-64.doi: 10.14088/j.cnki.issn0439-8114.2018.07.014

放线菌产杀虫物质的初步鉴别及其活性评价

张遵霞, 张亚妮, 方伟, 张志刚, 王开梅, 万中义

湖北省生物农药工程研究中心/湖北农业科技创新中心生物农药分中心,武汉 430064

收稿日期:2018-01-16 发布日期:2020-01-14
通讯作者: 万中义,研究员,博士,主要从微生物天然产物、生物农药的研究,（电话）027-59101928（电子信箱）wanzhongyi1964@126.com。
作者简介:张遵霞（1971-）,女,湖北武汉人,农艺师,主要从事生物农药研究,（电话）13971430512（电子信箱）1612336040@qq.com;
基金资助:
国家重点研发计划（2017YFD0201205）; 湖北省农业科技创新中心资助项目（2016-620-000-001-039）

Identification and Evaluation of a Natural Product with Insectcidal Activity Produced by an Actinomycetes Strain

ZHANG ZUN-xia,ZHANG Ya-ni, FANG Wei, ZHANG Zhi-gang, WANG Kai-mei, WAN Zhong-yi

Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesticide, Hubei Agricultural Sciences and Technology Innovation Center, Wuhan 430064, China

Received:2018-01-16 Published:2020-01-14

摘要/Abstract

摘要： 从中国四川乐山地区采集的土样中分离到1株放线菌HBERC-21542,农药活性测定发现其发酵粗提物对农业害虫具有很好的杀虫活性。对HPLC半制备组分进行农药活性测定结果表明,其杀虫活性成分出峰时间在14~19 min。根据HPLC-MS图谱特征确定了活性物质的分子离子峰及分子量,结合UV吸收光谱及文献数据,初步确定其为聚醚化合物费棱西霉素A。对发酵提取物进行HPLC制备后,获得了纯度在90%左右的纯品,在50 μg/mL浓度下,其对小菜蛾、棉铃虫的杀虫效果达到90%~100%。

关键词: 天然产物, 杀虫活性, 放线菌, 费棱西霉素A

Abstract: During the screening process of natural products produced by actionmycetes,a strain HBERC-21542 was isolated from soil samples collected in Leshan district,Sichuan province. The fermentation extract exhibited strong insectcidal activities against some insects such as Plutella xylostell,Helicoverpa armigera,etc. It was determined by components bioassay that the active compounds were in between 14~19 min in HPLC profile,and the active compound was identified as Ferensimycin A based on MS,UV absoption spectrum and natural product dictionary. Pure compound of 90% was obtained by preparative HPLC,and the lethal rate was 90%~100% at 50 μg/mL against Plutella xylostell,Helicoverpa armigera.

Key words: natural products, insectcidal activity, actinomycetes, Ferensimycin A

中图分类号:

S476

张遵霞, 张亚妮, 方伟, 张志刚, 王开梅, 万中义. 放线菌产杀虫物质的初步鉴别及其活性评价[J]. 湖北农业科学, 2018, 57(7): 61-64.

ZHANG ZUN-xia, ZHANG Ya-ni, FANG Wei, ZHANG Zhi-gang, WANG Kai-mei, WAN Zhong-yi. Identification and Evaluation of a Natural Product with Insectcidal Activity Produced by an Actinomycetes Strain[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(7): 61-64.

[1]	张遵霞, 王月莹, 张亚妮, 张志刚, 王开梅. Okaramine类生物碱——一类具有新颖杀虫机制的天然吲哚化合物[J]. 湖北农业科学, 2019, 58(23): 86-90.
[2]	吴兆圆, 张亚妮, 王燕, 方伟, 张志刚. 真菌NBERC_170302中次生代谢产物Viridicatumtoxin A的分离纯化及农药活性[J]. 湖北农业科学, 2018, 57(17): 56-58.
[3]	李玉春, 周富婷, 肖丰坤, 韦勇, 李彪, 周艳梅, 蒋水萍. 六种植物精油对赤拟谷盗的杀虫活性研究[J]. 湖北农业科学, 2018, 57(14): 49-52.

放线菌产杀虫物质的初步鉴别及其活性评价

Identification and Evaluation of a Natural Product with Insectcidal Activity Produced by an Actinomycetes Strain

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价