常压室温等离子体（ARTP）诱变快速选育对亚洲玉米螟高毒的苏云金芽孢杆菌突变株

doi:10.14088/j.cnki.issn0439-8114.2018.21.032

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (21): 123-126.doi: 10.14088/j.cnki.issn0439-8114.2018.21.032

常压室温等离子体（ARTP）诱变快速选育对亚洲玉米螟高毒的苏云金芽孢杆菌突变株

廖先清, 周荣华, 刘芳, 张志刚, 饶犇, 陈伟, 王开梅

湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心,武汉 430064

收稿日期:2018-08-14 出版日期:2018-11-10 发布日期:2020-01-13
作者简介:廖先清（1973-）,女,湖北随州人,副研究员,主要从事微生物农药的研究与产业化开发,（电话）13317174866（电子信箱）1120777387@qq.com
基金资助:
国家重点研发计划课题（2017YFD0201205）; 湖北省农业科技中心创新资金项目（2016-620-000-001-038）

Rapid Mutation Breeding of Highly Toxic Bacillus thuringiensis Strain Against Asian Corn Borer-by Atmospheric and Room Temperature Plasma

LIAO Xian-qing, ZHOU Rong-hua, LIU Fang, ZHANG Zhi-gang, RAO Ben, CHEN Wei, WANG Kai-mei

Hubei Biopesticide Engineering Research Center/Biopesticide Subcenter of Hubei Science and Technology Innovation Center,Wuhan 430064,China

Received:2018-08-14 Online:2018-11-10 Published:2020-01-13

摘要/Abstract

摘要： 采用常压室温等离子体（Atmospheric and room temperature plasma,ARTP）诱变苏云金芽孢杆菌Bt-NBIC-380菌株,选育对亚洲玉米螟高毒力突变株。经过4轮的ARTP诱变、镜检及摇瓶发酵筛选得到的突变株Bt-NBIC-380-18-407,其摇瓶发酵液对玉米螟二龄幼虫效价达到6 835 IU/μL,杀虫晶体蛋白含量6.3 mg/mL。该突变株的效价较初始菌株提高了25.7%,杀虫晶体蛋白含量提高了18.9%。经过5次传代,证实该突变株具有良好的遗传稳定性。

关键词: 常压室温等离子体（ARTP）诱变, 苏云金芽孢杆菌Bt-NBIC-380, 亚洲玉米螟, 杀虫晶体蛋白, 生物效价

Abstract: A highly toxic Bacillus thuringiensis strain NBIC-380 against Asian corn borer was treated by atmospheric and room temperature plasma for improving the toxicity of the resulting mutants. Through 4 rounds of ARTP mutation and screening by microscopic checking,shake-flask fermentation and bioassay,one mutant designated as Bt-NBIC-380-18-407 with significant improvement of production of insecticidal crystal protein and potency-was obtained. The potency of the fermentation broth of the mutant against Asian corn borer larvae was increased 25.7% to 6 835 IU/μl,while the concentration of insecticidal crystal protein in fermentation broth was increased 18.9% to 6.3 mg/mL. The mutant showed rather good genetic stability after 5 times of consecutive transferring and cultivation.

Key words: ARTP mutagenesis, Bacillus thuringiensis, Asian corn borer, insecticidal crystal proteins, potency

中图分类号:

S476.1

廖先清, 周荣华, 刘芳, 张志刚, 饶犇, 陈伟, 王开梅. 常压室温等离子体（ARTP）诱变快速选育对亚洲玉米螟高毒的苏云金芽孢杆菌突变株[J]. 湖北农业科学, 2018, 57(21): 123-126.

LIAO Xian-qing, ZHOU Rong-hua, LIU Fang, ZHANG Zhi-gang, RAO Ben, CHEN Wei, WANG Kai-mei. Rapid Mutation Breeding of Highly Toxic Bacillus thuringiensis Strain Against Asian Corn Borer-by Atmospheric and Room Temperature Plasma[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(21): 123-126.

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常压室温等离子体（ARTP）诱变快速选育对亚洲玉米螟高毒的苏云金芽孢杆菌突变株

Rapid Mutation Breeding of Highly Toxic Bacillus thuringiensis Strain Against Asian Corn Borer-by Atmospheric and Room Temperature Plasma

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