湖北农业科学 ›› 2018, Vol. 57 ›› Issue (21): 118-120.doi: 10.14088/j.cnki.issn0439-8114.2018.21.030

• 检测分析 • 上一篇    下一篇

石柱黄连主要生物碱的测定

司海倩1, 封海东1, 张振1, 柯磊1, 张泽志1, 李坤1, 周明1, 周军1, 王学奎2   

  1. 1.十堰市农业科学院,湖北 十堰 442000;
    2.华中农业大学植物科学技术学院,武汉 430070
  • 收稿日期:2018-01-13 出版日期:2018-11-10 发布日期:2020-01-13
  • 通讯作者: 王学奎(1965-),男,主要从事药用植物栽培、育种及其资源评价与利用研究,(电子信箱)wang-xuekui@mail.hzau.edu.cn。
  • 作者简介:司海倩(1986-),女,河南商丘人,农艺师,硕士,主要从事中药材育种及推广工作,(电话)15346601719(电子信箱)836623914@qq.com
  • 基金资助:
    中医药现代化研究重点专项(2017YFC1701001)

Quantitative Determination of the Main Alkaloids from Coptis chinensis in Shizhu

SI Hai-qian1, FENG Hai-dong1, ZHANG Zhen1, KE Lei1, ZHANG Ze-zhi1, LI Kun1, ZHOU Ming1, ZHOU Jun1, WANG Xue-kui2   

  1. 1.Shiyan Academy of Agricultural Sciences,Shiyan 442000,Hubei,China;
    2.College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2018-01-13 Online:2018-11-10 Published:2020-01-13

摘要: 采用高效液相色谱法(HPLC)测定黄连(Coptis chinensis)主要生物碱。结果表明,石柱不同栽培片区黄连不同部位主要生物碱含量均存在差异,且4种生物碱的含量高低顺序均为:小檗碱>黄连碱>表小檗碱>巴马汀,可为黄连规范化种植及其副产品的综合开发利用提供理论依据和应用参考价值。

关键词: 黄连(Coptis chinensis), 生物碱, 片区, 部位

Abstract: Through the determination and analysis of the main alkaloids of different parts of Coptis chinensis in different cultivation areas of Shizhu, the basis for reasonable planting and comprehensive development of Coptis chinensis was provided. Quantitative Determination of the main alkaloids from Coptis chinensis by HPLC methods. The main alkaloids of different parts of Coptis chinensis in different areas were differences of Sizhu. These four alkaloids’ content were in the order of epiberberine> coptisine> palmatine> berberine. The result canbe used to provide theoretical basis and application reference value for the comprehensive development and utilization of standardized planting and its by-products of Coptis chinensis.

Key words: Coptis chinensis, alkaloids, areas, parts

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