拟南芥耐硒突变体的鉴定及基因定位

doi:10.14088/j.cnki.issn0439-8114.2018.02.029

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (2): 115-118.doi: 10.14088/j.cnki.issn0439-8114.2018.02.029

拟南芥耐硒突变体的鉴定及基因定位

陈大清¹, 钟育海², 李应生, 苏爱国, 朱云芬⁴, 李亚男

1.仲恺农业工程学院农业与生物学院,广州 510225;
2.恩施州种子管理局,湖北恩施 445000;
3.长江大学,a.生命科学学院;b.发展规划处,湖北荆州 434023;
4.恩施州硒应用技术与产品开发研究院,湖北恩施 445000

收稿日期:2018-01-05 出版日期:2018-01-25 发布日期:2018-01-10
通讯作者: 李亚男,女,教授,主要从事作物生理学研究,（电话）18986660658（电子信箱）402995452@qq.com。
作者简介:陈大清（1962-）,男,湖北钟祥人,教授,博士,主要从事植物分子生物学研究,（电话）13560380388（电子信箱）daqingchen@vip.sina.com; 并列第一作者,钟育海（1983-）,男（土家族）,湖北恩施人,农艺师,硕士,主要从事农作物新品种管理及推广研究,（电话）15272222067 （电子信箱）zhongyuhai99@163.com
基金资助:
国家自然科学基金项目（31560579）; 广东省科技厅项目（2015A030302081）; 广东省教育厅人才引进项目（粤财教2010343号）; 广东省技术质量监督局（粤财农[2017]66号）

Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana

CHEN Da-qing¹, ZHONG Yu-hai², LI Ying-sheng, SU Ai-guo, ZHU Yun-fen⁴, LI Ya-nan

1.College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;
2.Enshi Seed Authority,Enshi 445000,Hubei,China;
3a.School of Life Science;3b.Development and Planning Department,Yangtze University,Jingzhou 434023,Hubei,China;
4.Enshi Institute of Selenium Application Technology and Product Development,Enshi 445000,Hubei,China

Received:2018-01-05 Online:2018-01-25 Published:2018-01-10

摘要/Abstract

摘要： 在硒盐胁迫下通过根弯曲特性筛选获得拟南芥（Arabidopsis thaliana）耐硒突变体,并以突变体为母本与野生型（Ler）杂交获得基因型完全杂合的F₁代;自交产生的F₂代硒敏感,硒耐受的分离比为3∶1,表明该突变体是隐性单基因遗传。F₂代选出132株具有耐硒特性的植株作为图位克隆的群体,从应用SSLP和In/Del分子标记进行基因定位,通过分析目的基因与各分子标记之间的连锁关系,发现耐硒突变体基因位于第I号染色体F12P19和NGA111的分子标记之间。

关键词: 拟南芥（Arabidopsis thaliana）, 硒盐胁迫, 突变体, 基因定位

Abstract: A selenate-torlerance arabidopsis mutant was obtained by using the root-bending trait under selenate stress. It was conformed that the selenate resistant characteristics could be steady inheritance. Then this mutant was crossed with the wild-type Ler. and F₁ generation were created with heterozygous type completely. The F₂ crossed by theirself were expressed the separation ratio of 3∶1 in response of sensitive selenium to resistent selenium,It were suggested that this mutant was a monogenic recessive mutation. Map-based cloning population were composed of the 132 selenate-tolerance seedlings in F₂ generation. Using SSLP and In/Del based on PCR to preliminary chromosomal location. Through linkage analysis,it's indicated that this mutant gene was located on the chromosome I,which was mapped between molecular marker F12P19 and NGA111.

Key words: Arabidopsis thaliana, selenate stress, mutant, gene lacation

中图分类号:

陈大清, 钟育海, 李应生, 苏爱国, 朱云芬, 李亚男. 拟南芥耐硒突变体的鉴定及基因定位[J]. 湖北农业科学, 2018, 57(2): 115-118.

CHEN Da-qing, ZHONG Yu-hai, LI Ying-sheng, SU Ai-guo, ZHU Yun-fen, LI Ya-nan. Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(2): 115-118.

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拟南芥耐硒突变体的鉴定及基因定位

Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana

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