野桑蚕scalloped同源基因的克隆和序列分析

doi:10.14088/j.cnki.issn0439-8114.2019.17.033

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (17): 123-128.doi: 10.14088/j.cnki.issn0439-8114.2019.17.033

野桑蚕scalloped同源基因的克隆和序列分析

朱文东

安康学院现代农业与生物科技学院/陕西省蚕桑重点实验室,陕西安康 725000

收稿日期:2019-03-04 发布日期:2019-11-14
作者简介:朱文东（1972-）,男,陕西紫阳人,副教授,硕士,主要从事农业优质高效新理论和新技术研究,（电话）15991197772（电子信箱）zhwd04@aku.edu.cn。
基金资助:
陕西省教育厅重点实验室科研计划项目（18JS002; 16JS001）; 陕南秦巴山区生物资源综合开发协同创新中心项目（QBXT-Z（Z）-15-5）; 安康学院硕士点培育学科建设专项（2016AYXNZX018）

Cloning and sequence analysis of scalloped homologous gene of wild silkworm

ZHU Wen-dong

College of Modern Agriculture and Biotechnology,Ankang University/Shaanxi Key Laboratory of Sericulture,Ankang 725000,Shaanxi,China

Received:2019-03-04 Published:2019-11-14

摘要/Abstract

摘要： Hippo通路与昆虫的生长、发育和变态等过程密切相关,在动物不同物种之间高度保守。scalloped基因是Hippo通路元件Yki的偶联因子之一,参与调控基因的表达。克隆了野桑蚕（Bombyx mandarina）scalloped基因的完整开放阅读框（ORF）及其上下游的部分非编码序列。序列联配分析表明,与家蚕相比,野桑蚕scalloped基因序列存在多处的核酸片段缺失、插入及位点差异。结构域分析表明,野桑蚕Scalloped蛋白与家蚕、黑腹果蝇、小鼠和人的蛋白结构类似,均含有完整的TEAD蛋白家族典型TEA结构域和PDB结构域,但野桑蚕编码蛋白缺失蛋白C末端序列。系统发生树分析表明,昆虫Scalloped蛋白与脊椎动物TEF3可能存在共同的起源;家蚕Scalloped在进化中出现晚于野桑蚕且经历较多遗传选择过程。

关键词: 野桑蚕（Bombyx mandarina）, Hippo通路, scalloped基因

Abstract: Hippo pathway is closely related to the physiological and biochemical processes of insect growth, metamorphosis and development, and highly conserved among different species. Scalloped is one of terminal action factor of Hippo pathway, which regulates the expression of a large number of genes. In this study, the complete ORF and partial UTR sequence of scalloped in wild silkworm （Bombyx mandarina） were cloned. Indels and variable sites were discovered after sequences analysis between Bombyx mori and Bombyx mandarina. The domain structure analysis shown that the encoding protein of scalloped in wild silkworm, domestic silkworm, fruit fly, mice and human have the similar domain architecture, while partial of the C-terminal of wild silkworm scalloped protein absent. The phylogenetic analysis showed that the Scalloped in insects and TEF3 in vertebrate could have a common origin, and the Scalloped of domestic silkworm could be appeared later than that in wild silkworm, and experienced more genetic selection process.

Key words: wild silkworm（Bombyx mandarina）, Hippo pathway, scalloped gene

中图分类号:

Q785

朱文东. 野桑蚕scalloped同源基因的克隆和序列分析[J]. 湖北农业科学, 2019, 58(17): 123-128.

ZHU Wen-dong. Cloning and sequence analysis of scalloped homologous gene of wild silkworm[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(17): 123-128.

参考文献

[1] BADOUEL C,GARG A,MCNEILL H.Herding Hippos:Regulating growth in flies and man[J].Current opinion in cell biology,2009,21(6):837-843.
[2] PANTALACCI S,TAPON N,LEOPOLD P.The Salvador partner Hippo promotes apoptosis and cell-cycle exit in Drosophila[J]. Nat Cell Biol,2003,5(10):921-927.
[3] UDAN R S,KANGO-SINGH M,NOLO R,et al.Hippo promotes proliferation arrest and apoptosis in the Salvador/Warts pathway[J].Nat Cell Biol,2003,5(10):914-920.
[4] XU T,WANG W,ZHANG S,et al.Identifying tumor suppressors in genetic mosaics:The Drosophila lats gene encodes a putative protein kinase[J].Development,1995,121(4):1053-1063.
[5] HARVEY K F,PFLEGER C M,HARIHARAN I K.The Drosophila Mst ortholog, hippo,restricts growth and cell proliferation and promotes apoptosis[J].Cell,2003,114(4):457-467.
[6] WU S,HUANG J,DONG J,et al.hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and warts[J].Cell,2003, 114(4):445-456.
[7] KANGO-SINGH M,NOLO R,TAO C,et al.Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila[J].Development,2002,129(24):5719-5730.
[8] TAPON N,HARVEY KF,BELL D W,et al.salvador promotes both cell cycle exit and apoptosis in Drosophila and is mutated in human cancer cell lines[J].Cell,2002,110(4):467-478.
[9] ZHANG L.Control of growth and beyond:A special issue on Hippo signaling[J].Acta Biochim Biophys Sin(Shanghai),2015, 47(1):1.
[10] DONG J,FELDMANN G,HUANG J,et al.Elucidation of a universal size-control mechanism in Drosophila and Mammals[J].Cell,2007,130(6):1120-1133.
[11] ZHOU Y,HUANG T,CHENG A S,et al.The TEAD family and its oncogenic role in promoting tumorigenesis[J].International journal of molecular sciences,2016,17(2):138.
[12] LAMAR J M,STERN P,LIU H,et al.The Hippo pathway target,YAP,promotes metastasis through its TEAD-interaction domain[J].Proceedings of the national academy of sciences,2012,109(37):E2441-E2450.
[13] YIN M X,ZHANG L.Hippo signaling in epithelial stem cells[J].Acta biochimica biophysica sinica,2015,47(1):39-45.
[14] SHI P,FENG J,CHEN C.Hippo pathway in mammary gland development and breast cancer[J].Acta biochimica biophysica sinica,2015,47(1):53-59.
[15] MO J S,MENG Z,KIM Y C,et al.Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway[J].Nature cell biology,2015,17(4):500-510.
[16] MAO B,GAO Y,BAI Y,et al.Hippo signaling in stress response and homeostasis maintenance[J].Acta biochimica biophysica sinica,2015,47(1):2-9.
[17] XIANG H,LIU X,LI M,et al.The evolutionary road from wild moth to domestic silkworm[J].Nature ecology & evolution,2018,2(8):1268-1279.
[18] XIA Q,GUO Y,ZHANG Z,et al.Complete resequencing of 40 genomes reveals domestication events and genes in silkworm(Bombyx)[J].Science,2009,326(5951):433-436.
[19] XU X,BI H L,ZHANG Z J,et al.BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm,Bombyx mori[J].Insect science,2018,25(6):1006-1016.
[20] ZENG W,LIU R,ZHANG T,et al.BmYki is transcribed into four functional splicing isoforms in the silk glands of the silkworm Bombyx mori[J].Gene,2017,646:39-46.

野桑蚕scalloped同源基因的克隆和序列分析

Cloning and sequence analysis of scalloped homologous gene of wild silkworm

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