湖北农业科学 ›› 2019, Vol. 58 ›› Issue (17): 116-118.doi: 10.14088/j.cnki.issn0439-8114.2019.17.031

• 检测分析 • 上一篇    下一篇

商洛桔梗茎叶中桔梗皂苷D动态研究

赵志新, 王通, 潘苗   

  1. 商洛学院生物医药与食品工程学院,陕西 商洛 726000
  • 收稿日期:2019-03-05 发布日期:2019-11-14
  • 作者简介:赵志新(1982-),男,河南滑县人,讲师,博士,主要从事植物转录组代谢及基因组进化研究,(电话)15829967656(电子信箱)zxzhao@slxy.edu.cn。
  • 基金资助:
    陕西省教育厅2018年度专项科学研究计划项目(18JK0251)

Dynamic of platycodin D in stems and leaves of Platycodon grandiflorum from Shangluo

ZHAO Zhi-xin, WANG Tong, PAN Miao   

  1. College of Biopharmaceutical and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China
  • Received:2019-03-05 Published:2019-11-14

摘要: 采用高效液相色谱法(HPLC)分析商洛种植的两年生桔梗(Platycodon grandiflorum)茎和叶在不同月份(5—10月)中桔梗皂苷D的含量变化,以探究主要次生代谢产物在桔梗茎叶中积累量的变化。结果表明,5—10月桔梗皂苷D的含量在茎中比较稳定,9月份达到最大值;而叶中桔梗皂苷D含量在7—8月份含量较高,10月份含量急剧下降;叶中桔梗皂苷D平均含量(0.614%)大于茎中(0.257%),表明商洛地区所种植桔梗幼嫩茎叶也可入药,其采收期应在8月份。通过对不同月份及茎叶中桔梗皂苷D含量的研究,可为确定商洛桔梗入药部位及采收期提供参考,同时也可为桔梗质量控制及规范化种植提供借鉴。

关键词: 桔梗(Platycodon grandiflorum), HPLC, 桔梗皂苷D, 不同月份, 茎叶

Abstract: High performance liquid chromatography (HPLC) was utilized to analyze the variations of Platycodin D content in stems and leaves in Platycodon grandiflorum (Jacq.) A. DC., which were collected from May to October from Shangluo area, and the study was aimed to explore the variations of main secondary metabolites in stems and leaves. The results showed that the contents of Platycodin D were stably in the stems from May to October, and the peak was reached in September; the contents of Platycodin D were highly in July and August, yet the content decreased dramatically in October. Furthormore, the average content of Platycodin D in leaves (0.614%) was higher than in stems (0.257%). Therefore, the young stems and leaves also could be used as medicines, and their harvest time should be August in Shangluo. By investigating the variations of Platycodin D content in stems and leaves in different months, the study can provide references for medicinal parts and harvest time and standardization production in P. grandiflorum in Shangluo.

Key words: Platycodon grandiflorum, HPLC, platycodin D, different months, stems and leaves

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