湖北农业科学 ›› 2018, Vol. 57 ›› Issue (3): 75-77.doi: 10.14088/j.cnki.issn0439-8114.2018.03.017

• 贮藏·加工 • 上一篇    下一篇

新疆胡杨树皮提取木聚糖的研究

王晶, 董翔, 覃瑞, 熊海容   

  1. 中南民族大学生命科学学院,武汉 430074
  • 收稿日期:2017-11-14 出版日期:2018-02-10 发布日期:2019-12-25
  • 通讯作者: 熊海容(1966-),男,教授,博士,主要从事酶工程研究,(电子信箱)xionghr@mail.scuec.edu.cn。
  • 作者简介:王晶(1995-),女,湖北武汉人,在读硕士研究生,研究方向为酶工程,(电话)15392940746(电子信箱)15392940746@163.com。
  • 基金资助:
    中央高校科研基金项目(CZP17068)

Study on Extraction of Xylan from Xinjiang Populus Bark

WANG Jing, DONG Xiang, QIN Rui, XIONG Hai-rong   

  1. College of Life Sciences, South-Central University for Nationalities, Wuhan 430074,China
  • Received:2017-11-14 Online:2018-02-10 Published:2019-12-25

摘要: 采集新疆地区胡杨(Populus euphratica)树皮,利用碱提法提取其木聚糖。采用水煮处理脱去大部分水溶性物质后,在胡杨树皮中加入10%氢氧化钠溶液(mV=1∶5)在70 ℃下碱提1 h,8层纱布过滤后的碱提滤液加入0.15倍体积的30%双氧水,70 ℃脱色1 h,最后调pH至中性,加入2倍体积乙醇,醇沉获得木聚糖。结果表明,得到木聚糖的干重收率为4.35%,自提胡杨树皮木聚糖能够作为木聚糖酶试验中的底物使用,具有一定的替代性,且其来源为新疆胡杨树皮,对当地资源开发利用有一定实际意义。

关键词: 胡杨(Populus euphratica), 资源利用, 新疆, 木聚糖

Abstract: The bark of Populus euphratica in Xinjiang was collected,and then extracted xylan by alkali extraction method. The sediment of the bark of Populus euphratica was cleaned up and most of the water-soluble substance was washed away with boiled water. After adding the 10% sodium hydroxide solution(mV=1∶5) at 70 ℃ for 1 h,the alkali extract which was filtered by eight-layer gauze was added with 0.15 fold volume of 30% of hydrogen peroxide at 70 ℃ for 1 h,for decolorization. The final pH was adjusted to neutral,with ethanol(VV=1∶2) to obtain xylan. The dry-based yield was 4.35%. The results showed that the xylan from Populus euphratica could be used as a substrate in xylanase experiment.

Key words: populus(Populus euphratica), resource utilization, Xinjiang, xylan

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